Sven Becker1, Julia L Zimmermann2, Philipp Baumeister3, Theresa F Brunner3, Tetsuji Shimizu2, Yang-Fang Li2, Gregor E Morfill2, Ulrich Harréus4, Christian Welz5. 1. Department of Otorhinolaryngology, Head and Neck Surgery, University Medical Center, Johannes Gutenberg University, Langenbeckstr. 1, 55101 Mainz, Germany; University of Munich, Marchioninistr. 15, 81377 Munich, Germany. Electronic address: sven.becker@unimedizin-mainz.de. 2. Terraplasma GmbH, Lichtenbergstr. 8, 85741 Garching, Germany. 3. University of Munich, Marchioninistr. 15, 81377 Munich, Germany. 4. University of Munich, Marchioninistr. 15, 81377 Munich, Germany; Department of Otorhinolaryngology, Head and Neck Surgery, Evangelisches Krankenhaus Düsseldorf, Kirchfeldstrasse 40, 40217 Düsseldorf, Germany. 5. University of Munich, Marchioninistr. 15, 81377 Munich, Germany; Department of Otorhinolaryngology, Head and Neck Surgery, Georg-August-University of Göttingen, Robert-Koch-Strasse 40, 37075 Göttingen, Germany.
Abstract
OBJECTIVE: Ear, nose and throat infections are among the most common reasons for absence from work. They are usually caused by various bacteria like Haemophilus influenzae, Staphylococcus aureus, Streptococcus pneumoniae and Streptococcus pyogenes. Cold atmospheric plasma (CAP) can effectively eliminate even multi-resistant bacteria and has no cytotoxic or mutagenic effects on the mucosa when applied for less than 60s. Aim of the study was to evaluate the effects of CAP on common ENT bacteria and on the mucosa of the upper aerodigestive tract. METHODS: The bactericidal effects of CAP against the bacteria most commonly causing ENT infections were investigated using the colony-forming units assay (CFU) on a Müller-Hinton agar plate after applying CAP for 30, 60, 90 and 120s. To evaluate the interaction of CAP with mucosal cells, 3D mini organ cultures were treated for up to 180s, after which cell viability and necrosis induction were evaluated. RESULTS: Treatment with CAP for 60s or longer induced at least a 3-log10 reduction in the bacterial load (> 99.9%). Treatment times shorter than 60s had only slight cytotoxic effects on cell viability and necrosis whereas treatment times above 60s showed a fast increase of cytotoxic side effects. CONCLUSION: CAP exhibited strong bactericidal effects on the most common ENT pathogens. Treatment times of up to 60s showed only minimal adverse reactions in healthy mucosa. CAP could be a promising new therapeutic modality for ENT infections.
OBJECTIVE: Ear, nose and throat infections are among the most common reasons for absence from work. They are usually caused by various bacteria like Haemophilus influenzae, Staphylococcus aureus, Streptococcus pneumoniae and Streptococcus pyogenes. Cold atmospheric plasma (CAP) can effectively eliminate even multi-resistant bacteria and has no cytotoxic or mutagenic effects on the mucosa when applied for less than 60s. Aim of the study was to evaluate the effects of CAP on common ENT bacteria and on the mucosa of the upper aerodigestive tract. METHODS: The bactericidal effects of CAP against the bacteria most commonly causing ENT infections were investigated using the colony-forming units assay (CFU) on a Müller-Hinton agar plate after applying CAP for 30, 60, 90 and 120s. To evaluate the interaction of CAP with mucosal cells, 3D mini organ cultures were treated for up to 180s, after which cell viability and necrosis induction were evaluated. RESULTS: Treatment with CAP for 60s or longer induced at least a 3-log10 reduction in the bacterial load (> 99.9%). Treatment times shorter than 60s had only slight cytotoxic effects on cell viability and necrosis whereas treatment times above 60s showed a fast increase of cytotoxic side effects. CONCLUSION: CAP exhibited strong bactericidal effects on the most common ENT pathogens. Treatment times of up to 60s showed only minimal adverse reactions in healthy mucosa. CAP could be a promising new therapeutic modality for ENT infections.
Authors: Felix Theinkom; Larissa Singer; Fabian Cieplik; Sylvia Cantzler; Hannes Weilemann; Maximilian Cantzler; Karl-Anton Hiller; Tim Maisch; Julia L Zimmermann Journal: PLoS One Date: 2019-11-26 Impact factor: 3.240
Authors: Peter P Sun; Jungeun Won; Gabrielle Choo-Kang; Shouyan Li; Wenyuan Chen; Guillermo L Monroy; Eric J Chaney; Stephen A Boppart; J Gary Eden; Thanh H Nguyen Journal: NPJ Biofilms Microbiomes Date: 2021-06-02 Impact factor: 7.290