Krishnadas Madhu1, Prakash T2. 1. Department of Pharmacology, Acharya & BM Reddy College of Pharmacy, Bengaluru, India. 2. Department of Pharmacology, Acharya & BM Reddy College of Pharmacy, Bengaluru, India. Electronic address: prakasht@acharya.ac.in.
Abstract
BACKGROUND: During the development of Multiple Sclerosis (MS) there is a marked activation of microglia and astrocyte, leading to progressive inflammation and degeneration of myelin sheath which results in axonal loss and neuron damage. PURPOSE: In this study, we have explored the action of Asiaticoside A against the activated microglia and astrocytes. METHODS: Primary microglia and astrocyte cultures were used for the study and the activity were evaluated using cell proliferation assay, nitrate assay and TNFα estimation using ELISA. RESULTS: Asiaticoside A inhibited the production of nitric oxide and TNFα in LPS activated primary microglia and astrocyte cultures. CONCLUSION: This study suggests that Asiaticoside A may be effective against the progression of MS.
BACKGROUND: During the development of Multiple Sclerosis (MS) there is a marked activation of microglia and astrocyte, leading to progressive inflammation and degeneration of myelin sheath which results in axonal loss and neuron damage. PURPOSE: In this study, we have explored the action of Asiaticoside A against the activated microglia and astrocytes. METHODS: Primary microglia and astrocyte cultures were used for the study and the activity were evaluated using cell proliferation assay, nitrate assay and TNFα estimation using ELISA. RESULTS:Asiaticoside A inhibited the production of nitric oxide and TNFα in LPS activated primary microglia and astrocyte cultures. CONCLUSION: This study suggests that Asiaticoside A may be effective against the progression of MS.