A miniaturized solid-phase extraction adsorbent of calix[4]arene-functionalized graphene oxide/polydopamine-coated cellulose acetate for the analysis of aflatoxins in corn.

A calix[4]arene-functionalized graphene oxide/polydopamine-coated cellulose acetate adsorbent was fabricated for the pre-concentration of aflatoxins. The highly porous developed adsorbent does not produce the high backpressure that normally occurs in particle-packed cartridges and its large surface area helps to improve adsorption. The highly efficient adsorption of aflatoxins by the hybrid adsorbent is facilitated via hydrogen bonding and hydrophobic and π-π interactions. Polymerization time, amount of calix[4]arene-functionalized graphene oxide, type and volume of desorption solvent, sample pH, sample volume, and sample flow rate were optimized. The linearity of aflatoxin B1 was in the range of 0.01-10.0 μg/kg, aflatoxin B2 was in the range of 0.02-10.0 μg/kg and aflatoxin G1 and aflatoxin G2 were in the range of 0.050-10.0 μg/kg. The limits of detection were 0.01 μg/kg for aflatoxin B1, 0.02 μg/kg for aflatoxin B2 and 0.05 μg/kg for aflatoxin G1 and aflatoxin G2. The developed calix[4]arene-functionalized graphene oxide/polydopamine-coated cellulose acetate adsorbent was successfully utilized for the analysis of aflatoxins from corn samples and the extraction efficiency was satisfactory with obtained recoveries from 83.0 to 106.7%. Moreover, fabricated adsorbent is easy to prepare, inexpensive, and can be reused.