Luciano Ramos1, Rodrigo Labat Marcos2, Romildo Torres-Silva3, Rodney Capp Pallota2, Tatiana Magacho1, Fernando Francisco Pazello Mafra3, Michel Monteiro Macedo3, Rodrigo Leal de Paiva Carvalho4, Jan Magnus Bjordal5, Rodrigo Alvaro B Lopes-Martins6. 1. 1 Faculdade Pitágoras Guarapari-Rod, Governador Jones dos Santos Neves , Guarapari, Espírito Santo, Brazil . 2. 2 Postgraduate Program in Biophotonics Applied to Health Sciences, Universidade Nove de Julho , São Paulo, Brazil . 3. 3 Postgraduate Program in Biomedical Engineering, Technological Research Center-NPT, Universidade de Mogi das Cruzes (UMC) , Mogi das Cruzes, Brazil . 4. 4 Laboratório de Pesquisa em Fisioterapia, PPG em Fisioterapia, Universidade do Sagrado Coração , Bauru, São Paulo, Brazil . 5. 5 Department of Health Sciences, University of Bergen , Bergen, Norway . 6. 6 Instituto de Pesquisa and Desenvolvimento, Universidade do Vale do Paraíba (UNIVAP) , São José dos Campos, São Paulo, Brazil .
Abstract
BACKGROUND: Unusual and exhaustive physical exercise can lead to muscle lesions depending on the type of contraction, intensity, duration, age, and level of conditioning. Different therapies have been proposed to prevent or reduce exercise-induced muscle damage. OBJECTIVE: In this study, we investigate the effects of low-level laser therapy on skeletal muscle strain in an experimental model in rats. MATERIALS AND METHODS: Male Wistar rats (200 g) were used. The animals were randomized into groups of six animals. We performed tibialis muscle elongation using a previously described protocol. The animals were anesthetized and submitted to passive stretching of the anterior tibial muscle attached to a weight corresponding to 150% of the body mass of the animal for 20 min, rested for 3 min, and received a second traction for 20 min. The cytokines, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), IL-6, and IL-10, edema, and C-reactive protein (CRP) levels were determined in the tibialis anterior muscle. RESULTS: Plasma extravasation of groups treated with different doses of laser energy, lesion +1 J (2.61 ± 0.46), lesion +3 J (2.33 ± 0.13), lesion +6 J (2.92 ± 0.91), and lesion +9 J (2.80 ± 0.55), shows a significant reduction of extravasation when compared with the injury group (5.46 ± 1.09). Laser therapy was able to significantly reduce CRP and cytokine levels (TNF-α, IL-1β, IL-6, and IL-10). CONCLUSIONS: Laser photobiomodulation reduced skeletal muscle edema as well as cytokines and CRP, leading to a significant reduction in inflammatory markers.
BACKGROUND: Unusual and exhaustive physical exercise can lead to muscle lesions depending on the type of contraction, intensity, duration, age, and level of conditioning. Different therapies have been proposed to prevent or reduce exercise-induced muscle damage. OBJECTIVE: In this study, we investigate the effects of low-level laser therapy on skeletal muscle strain in an experimental model in rats. MATERIALS AND METHODS: Male Wistar rats (200 g) were used. The animals were randomized into groups of six animals. We performed tibialis muscle elongation using a previously described protocol. The animals were anesthetized and submitted to passive stretching of the anterior tibial muscle attached to a weight corresponding to 150% of the body mass of the animal for 20 min, rested for 3 min, and received a second traction for 20 min. The cytokines, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), IL-6, and IL-10, edema, and C-reactive protein (CRP) levels were determined in the tibialis anterior muscle. RESULTS: Plasma extravasation of groups treated with different doses of laser energy, lesion +1 J (2.61 ± 0.46), lesion +3 J (2.33 ± 0.13), lesion +6 J (2.92 ± 0.91), and lesion +9 J (2.80 ± 0.55), shows a significant reduction of extravasation when compared with the injury group (5.46 ± 1.09). Laser therapy was able to significantly reduce CRP and cytokine levels (TNF-α, IL-1β, IL-6, and IL-10). CONCLUSIONS: Laser photobiomodulation reduced skeletal muscle edema as well as cytokines and CRP, leading to a significant reduction in inflammatory markers.
Authors: Dennis Muñoz-Vergara; Weronika Grabowska; Gloria Y Yeh; Sat Bir Khalsa; Kristin L Schreiber; Christene A Huang; Ann Marie Zavacki; Peter M Wayne Journal: PLoS One Date: 2022-06-01 Impact factor: 3.752