Muhammad Hafeez1, Sisi Liu2, Saad Jan3, Bahar Ali1, Muhammad Shahid4, G Mandela Fernández-Grandon5, Muhammad Nawaz1, Aqeel Ahmad1, Mo Wang1. 1. Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University. 2. College of Science, Huazhong Agricultural University Wuhan, Hubei, China. 3. Bacha Khan University Charsadda, Department of Agriculture Entomology. 4. Guandong Public Laboratory of Wild Animal Conservation and Utilization, Guangdong Key Laboratory of Animal Protection and Resource Utilization, Guangdong Institute of Applied Biological Resources, Guangzhou, China. 5. Natural Resources Institute, University of Greenwich, Chatham, UK.
Abstract
BACKGROUND: In plant-insect interactions, phytotoxins such as gossypol, exert a defensive role on behalf of the plant by interfering with the essential metabolic, biochemical and physiological pathways of herbivorous insects. The beet armyworm, Spodoptera exigua (Hübner), is a key pest for many important crops including a range of vegetables, ornamentals, and cotton. In this study, we investigated how resistance to deltamethrin relates to enzyme activity in gossypol-pretreated larvae of S. exigua. RESULTS: Following selection with deltamethrin insecticides on gossypol-pretreated larvae for 10 generations, the Gos-SEL population developed a 113.29-fold resistance. Under the same conditions, the Delta-SEL selected population showed a 69.76-fold increase in resistance along with corresponding levels of xenobiotic defense enzyme activity. Similarly, the fecundity of the Delta-SEL population together with male and female longevity were found to be significantly lower when compared with the Gos-SEL population and the laboratory susceptible-strain group (SS-Strain). In addition, the activities of cytochrome P450s in S. exigua were significantly enhanced when the insects were fed on a deltamethrin and gossypol-pretreated diet compared with being fed on deltamethrin alone. CONCLUSION: The reproductive capacity of S. exigua is significantly reduced in Delta-SEL and Gos-SEL populations compared with the control group (SS-Strain). Elevation of the major detoxification enzyme cytochrome P450 monooxygenase and esterase might have an important role in inducing tolerance to deltamethrin in gossypol-fed S. exigua populations. This study enhances our understanding of detoxification enzyme pathways for S. exigua gene expression and their role in responses to insecticides and plant secondary metabolites.
BACKGROUND: In plant-insect interactions, phytotoxins such as gossypol, exert a defensive role on behalf of the plant by interfering with the essential metabolic, biochemical and physiological pathways of herbivorous insects. The beet armyworm, Spodoptera exigua (Hübner), is a key pest for many important crops including a range of vegetables, ornamentals, and cotton. In this study, we investigated how resistance to deltamethrin relates to enzyme activity in gossypol-pretreated larvae of S. exigua. RESULTS: Following selection with deltamethrin insecticides on gossypol-pretreated larvae for 10 generations, the Gos-SEL population developed a 113.29-fold resistance. Under the same conditions, the Delta-SEL selected population showed a 69.76-fold increase in resistance along with corresponding levels of xenobiotic defense enzyme activity. Similarly, the fecundity of the Delta-SEL population together with male and female longevity were found to be significantly lower when compared with the Gos-SEL population and the laboratory susceptible-strain group (SS-Strain). In addition, the activities of cytochrome P450s in S. exigua were significantly enhanced when the insects were fed on a deltamethrin and gossypol-pretreated diet compared with being fed on deltamethrin alone. CONCLUSION: The reproductive capacity of S. exigua is significantly reduced in Delta-SEL and Gos-SEL populations compared with the control group (SS-Strain). Elevation of the major detoxification enzyme cytochrome P450 monooxygenase and esterase might have an important role in inducing tolerance to deltamethrin in gossypol-fed S. exigua populations. This study enhances our understanding of detoxification enzyme pathways for S. exigua gene expression and their role in responses to insecticides and plant secondary metabolites.
Authors: Muhammad Hafeez; Saad Jan; Muhammad Nawaz; Ehsan Ali; Bahar Ali; Muhammad Qasim; G Mandela Fernández-Grandon; Muhammad Shahid; Mo Wang Journal: Environ Sci Pollut Res Int Date: 2019-03-12 Impact factor: 4.223
Authors: Junaid Ali Siddiqui; Muhammad Musa Khan; Bamisope Steve Bamisile; Muhammad Hafeez; Muhammad Qasim; Muhammad Tariq Rasheed; Muhammad Atif Rasheed; Sajjad Ahmad; Muhammad Ibrahim Shahid; Yijuan Xu Journal: Front Microbiol Date: 2022-05-03 Impact factor: 6.064
Authors: Muhammad Hafeez; Sisi Liu; Saad Jan; Le Shi; G Mandela Fernández-Grandon; Asim Gulzar; Bahar Ali; Muzammal Rehman; Mo Wang Journal: Int J Mol Sci Date: 2019-05-07 Impact factor: 5.923
Authors: Muhammad Hafeez; Xiaowei Li; Zhijun Zhang; Jun Huang; Likun Wang; Jinming Zhang; Sakhawat Shah; Muhammad Musa Khan; Fei Xu; G Mandela Fernández-Grandon; Myron P Zalucki; Yaobin Lu Journal: Insects Date: 2021-02-03 Impact factor: 2.769
Authors: Muhammad Hafeez; Muhammad Qasim; Sajjad Ali; Hafiz Kamran Yousaf; Muhammad Waqas; Ehsan Ali; Muhammad Afaq Ahmad; Saad Jan; Muhammad Amjad Bashir; Ali Noman; Mo Wang; Hamed A Gharmh; Khalid Ali Khan Journal: Saudi J Biol Sci Date: 2019-05-22 Impact factor: 4.219