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Literature DB >> 3009485

Site-specific OsO4 modification of the B-Z junctions formed at the (dA-dC)32 region in supercoiled DNA.

G Galazka, E Palecek, R D Wells, J Klysik.

Abstract

OsO4 in the presence of pyridine specifically modifies the structural distortions of the primary helix of supercoiled pRW777 near the (dA-dC)32 sequence. Modification occurs at the same negative superhelix density value as required for formation of the Z-helix within the polymer block. Fine mapping of the distorted regions, which are probably the B-Z junctions, is presented. OsO4 reactions provide a powerful and sensitive chemical approach to study DNA polymorphism in solution.

Entities: Chemical

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Year: 1986 PMID： 3009485

Source DB: PubMed Journal: J Biol Chem ISSN： 0021-9258 Impact factor: 5.157

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Cited

13 in total

1. Reaction conditions affect the specificity of bromoacetaldehyde as a probe for DNA cruciforms and B-Z junctions.

Authors: M J McLean; J E Larson; F Wohlrab; R D Wells
Journal: Nucleic Acids Res Date: 1987-09-11 Impact factor: 16.971

2. (A-T)n tracts embedded in random sequence DNA--formation of a structure which is chemically reactive and torsionally deformable.

Authors: J A McClellan; E Palecek; D M Lilley
Journal: Nucleic Acids Res Date: 1986-12-09 Impact factor: 16.971

3. The non-B-DNA structure of d(CA/TG)n differs from that of Z-DNA.

Authors: M P Kladde; Y Kohwi; T Kohwi-Shigematsu; J Gorski
Journal: Proc Natl Acad Sci U S A Date: 1994-03-01 Impact factor: 11.205

4. A versatile in vivo footprinting technique using 1,10-phenanthroline-copper complex to study important cellular processes.

Authors: S Basak; V Nagaraja
Journal: Nucleic Acids Res Date: 2001-11-01 Impact factor: 16.971

5. Both an altered DNA structure and cellular proteins are involved in protecting a triplex forming an oligopurine-rich sequence from Dam methylation in E. coli.

Authors: J Klysik
Journal: Biochem Genet Date: 1996-06 Impact factor: 1.890

6. Comparison of the reactivity of B-DNA and Z-DNA with two isosteric chemical carcinogens: 2-N,N-acetoxyacetylaminofluorene and 3-N,N-acetoxyacetylamino-4,6-dimethyldipyrido-[1,2-a:3',2' -d] imidazole.

Authors: L Marrot; E Hebert; G Saint-Ruf; M Leng
Journal: Nucleic Acids Res Date: 1987-07-24 Impact factor: 16.971

Site-specific OsO4 modification of the B-Z junctions formed at the (dA-dC)32 region in supercoiled DNA.

1. Reaction conditions affect the specificity of bromoacetaldehyde as a probe for DNA cruciforms and B-Z junctions.

2. (A-T)n tracts embedded in random sequence DNA--formation of a structure which is chemically reactive and torsionally deformable.

3. The non-B-DNA structure of d(CA/TG)n differs from that of Z-DNA.

4. A versatile in vivo footprinting technique using 1,10-phenanthroline-copper complex to study important cellular processes.

5. Both an altered DNA structure and cellular proteins are involved in protecting a triplex forming an oligopurine-rich sequence from Dam methylation in E. coli.

6. Comparison of the reactivity of B-DNA and Z-DNA with two isosteric chemical carcinogens: 2-N,N-acetoxyacetylaminofluorene and 3-N,N-acetoxyacetylamino-4,6-dimethyldipyrido-[1,2-a:3',2' -d] imidazole.

7. Parallel-stranded DNA under topological stress: rearrangement of (dA)15.(dT)15 to a d(A.A.T)n triplex.

8. Species-specific EcoRI repetitive elements of at least 16 kb in length are present in Lupinus luteus.

9. H-DNA and Z-DNA in the mouse c-Ki-ras promoter.

10. Intramolecular DNA triplexes in supercoiled plasmids.