Yuqian Tang1, Guirui Yu2, Xinyu Zhang3, Qiufeng Wang4, Dashuan Tian5, Jing Tian4, Shuli Niu4, Jianping Ge6. 1. Key Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China; College of Life Science, Beijing Normal University, Beijing 100875, China; College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100190, China. 2. Key Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China; College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100190, China. Electronic address: yugr@igsnrr.ac.cn. 3. Key Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China; College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100190, China. Electronic address: zhangxy@igsnrr.ac.cn. 4. Key Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China; College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100190, China. 5. Key Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China. 6. Key Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China; College of Life Science, Beijing Normal University, Beijing 100875, China.
Abstract
Because of increases in atmospheric nitrogen (N) deposition worldwide, nutrient imbalances and phosphorus (P) limitations in soil are aggravated, with the result that P fertilizer applications to terrestrial ecosystems worldwide may increase. Nitrification and denitrification in soil are major sources of nitrous oxide emissions, especially in soils treated with fertilizers. However, few researchers have studied how forest soils respond to nutrient additions, so we are not sure how the potential nitrification and denitrification activities (PNA and PDA, respectively) and microbial communities involved in these processes might respond when N and P are added to temperate and subtropical forest soils. We investigated how the PNA, PDA, the abundances and community compositions of nitrifiers and denitrifiers, and environmental properties, including soil pH, soil total and dissolved organic carbon, total and available N and phosphorus P, changed when N and/or P were added to subtropical and temperate forest soils. We quantified the abundance, and analyzed the composition, of functional marker genes of nitrifiers (ammonia-oxidizing bacteria and archaea amoA) and denitrifiers (nirK and nirS) using quantitative PCR and sequencing, respectively. We found that the PNA and PDA in the subtropical soil increased when P was added and PNA in the temperate forest soil increased when either N or P was added. The PNA and PDA were positively correlated with the abundance of ammonia-oxidizing bacteria and nirK-denitrifiers, respectively, in the subtropical forest soil but were not correlated with changes in corresponding community compositions in either of the forest soils. The soil total N to total P ratio explained most of the variabilities in the PNA and PDA in the subtropical forest soils, and the soil exchangeable ammonium concentrations and pH were the main controls on the PNA and PDA, respectively, in the temperate forest soils. Our results indicate that soil environmental conditions have more influence on variations in the PNA and PDA in forest soils fertilized with N and P than the corresponding microbial properties.
Because of increases in atmospheric nitrogen (pan> class="Chemical">N) deposition worldwide, nutrient imbalances and phosphorus (P) limitations in soil are aggravated, with the result that P fertilizer applications to terrestrial ecosystems worldwide may increase. Nitrification and denitrification in soil are major sources of nitrous oxide emissions, especially in soils treated with fertilizers. However, few researchers have studied how forest soils respond to nutrient additions, so we are not sure how the potential nitrification and denitrification activities (PNA and PDA, respectively) and microbial communities involved in these processes might respond when N and P are added to temperate and subtropical forest soils. We investigated how the PNA, PDA, the abundances and community compositions of nitrifiers and denitrifiers, and environmental properties, including soil pH, soil total and dissolved organic carbon, total and available N and phosphorus P, changed when N and/or P were added to subtropical and temperate forest soils. We quantified the abundance, and analyzed the composition, of functional marker genes of nitrifiers (ammonia-oxidizing bacteria and archaea amoA) and denitrifiers (nirK and nirS) using quantitative PCR and sequencing, respectively. We found that the PNA and PDA in the subtropical soil increased when P was added and PNA in the temperate forest soil increased when either N or P was added. The PNA and PDA were positively correlated with the abundance of ammonia-oxidizing bacteria and nirK-denitrifiers, respectively, in the subtropical forest soil but were not correlated with changes in corresponding community compositions in either of the forest soils. The soil total N to total P ratio explained most of the variabilities in the PNA and PDA in the subtropical forest soils, and the soil exchangeable ammonium concentrations and pH were the main controls on the PNA and PDA, respectively, in the temperate forest soils. Our results indicate that soil environmental conditions have more influence on variations in the PNA and PDA in forest soils fertilized with N and P than the corresponding microbial properties.