Objective: To explore the combined influence of Arisaematis Rhizoma polysaccharide with cisplatin on the proliferation,apoptosis and epithelial mesenchymal transition of breast carcinoma MDA-MB-231 cells. Methods: MDA-MB-231 cells were divided into control group,Arisaematis Rhizoma polysaccharide group( 50 μg / m L),cisplatin group( 5 μg / m L) and combined group( Arisaematis Rhizoma polysaccharide + cisplatin); the cells proliferation were detected by MTT assay, the cells apoptosis were detected by Annexin V / PI flow cytometry, the mRNA expression levels of Vimentin, N-cadherinand E-cadherin were detected by Real time PCR, the levels of Fibronectin( FN) were detected by ELISA,and the levels of Akt and p-Akt were measured by western blotting. Results: The proliferation of MDA-MB-231 cells were inhibited in Arisaematis Rhizoma polysaccharide group, cisplatin group and combined group with a time dependent manner. The early, late apoptosis rate and E-cadherin mRNA level in Arisaematis Rhizoma polysaccharide group, cisplatin group and combined group were higher,while Vimentin,N-cadherin mRNA,FN level and p-Akt / Akt were lower than those in control group( P < 0. 05). Compared with Arisaematis Rhizoma polysaccharide group and cisplatin group, there were higher in the early,late apoptosis rate and E-cadherin mRNA levels, lower the mRNA levels of Vimentin, N-cadherin, FN and p-Akt / Akt in combined group( P <0. 05). Conclusion: Both of Arisaematis Rhizoma polysaccharide and cisplatin can affect the proliferation, apoptosis and epithelial-mesenchymal transition, and inhibit activation of PI3 K / Akt signaling pathway and the combined effect is better.
Objective: To explore the combined influence of Arisaematis Rhizoma polysaccharide with cisplatin on the proliferation,apoptosis and epithelial mesenchymal transition of breast carcinoma MDA-MB-231 cells. Methods: MDA-MB-231 cells were divided into control group,Arisaematis Rhizoma polysaccharide group( 50 μg / m L),cisplatin group( 5 μg / m L) and combined group( Arisaematis Rhizoma polysaccharide + cisplatin); the cells proliferation were detected by MTT assay, the cells apoptosis were detected by Annexin V / PI flow cytometry, the mRNA expression levels of Vimentin, N-cadherinand E-cadherin were detected by Real time PCR, the levels of Fibronectin( FN) were detected by ELISA,and the levels of Akt and p-Akt were measured by western blotting. Results: The proliferation of MDA-MB-231 cells were inhibited in Arisaematis Rhizoma polysaccharide group, cisplatin group and combined group with a time dependent manner. The early, late apoptosis rate and E-cadherin mRNA level in Arisaematis Rhizoma polysaccharide group, cisplatin group and combined group were higher,while Vimentin,N-cadherin mRNA,FN level and p-Akt / Akt were lower than those in control group( P < 0. 05). Compared with Arisaematis Rhizoma polysaccharide group and cisplatin group, there were higher in the early,late apoptosis rate and E-cadherin mRNA levels, lower the mRNA levels of Vimentin, N-cadherin, FN and p-Akt / Akt in combined group( P <0. 05). Conclusion: Both of Arisaematis Rhizoma polysaccharide and cisplatin can affect the proliferation, apoptosis and epithelial-mesenchymal transition, and inhibit activation of PI3 K / Akt signaling pathway and the combined effect is better.