| Literature DB >> 30086191 |
Yingche Chen1, Kelvin Tsao1, Sydney L Acton1, Jeffrey W Keillor1.
Abstract
We report the development of YC23, a novel green BODIPY-based dimaleimide derivative that undergoes a fluorogenic addition reaction (FlARe) with a genetically encodable peptide tag (dC10α) that can be fused to a protein of interest (POI). We also demonstrate the application of this reaction for the fluorogenic labelling of a specific POI in bacterial lysate and in living mammalian cells.Entities:
Keywords: BODIPY; fluorogenic probes; maleimide; protein labelling
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Year: 2018 PMID： 30086191 DOI： 10.1002/anie.201805482
Source DB: PubMed Journal: Angew Chem Int Ed Engl ISSN： 1433-7851 Impact factor: 15.336