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Literature DB >> 3008587

Isolation of ADP-ribosyltransferase by affinity chromatography.

H J Burtscher, B Auer, H Klocker, M Schweiger, M Hirsch-Kauffmann.

Abstract

An affinity adsorbent for ADP-ribosyltransferase (EC 2.4.2.30) has been synthesized by coupling 3-aminobenzamide to Sepharose 4B. Using this material, ADP-ribosyltransferase from human placenta has been purified from crude extract to homogeneity within a few hours. The enzyme has an apparent Km for NAD+ of 52 microM. Its molecular mass is 115,000 as determined by gel electrophoresis. The enzyme is DNA dependent and stimulated by histone, its temperature optimum is at 25 degrees C, and its pH optimum is around pH 9. alpha-NAD+, thymidine, caffeine, theophylline, theobromine, 3-methoxybenzamide, and nicotinamide inhibit the enzyme. Purification of ADP-ribosyltransferases from horse, rat, and chicken liver was also achieved with the method described.

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Year: 1986 PMID： 3008587 DOI： 10.1016/0003-2697(86)90410-0

Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365

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Cited

11 in total

1. No abnormalities in the NAD+ ADP-ribosyltransferase (polymerizing) gene of transformed cells from a Fanconi's anemia patient.

Authors: K Flick; R Schneider; B Auer; M Hirsch-Kauffmann; M Schweiger
Journal: Hum Genet Date: 1992-08 Impact factor: 4.132

2. Binding of NAD+ by cholera toxin.

Authors: T S Galloway; S van Heyningen
Journal: Biochem J Date: 1987-05-15 Impact factor: 3.857

3. ADP-ribosyltransferase from Helix pomatia. Purification and characterization.

Authors: H J Burtscher; H Klocker; R Schneider; B Auer; M Hirsch-Kauffmann; M Schweiger
Journal: Biochem J Date: 1987-12-15 Impact factor: 3.857

4. Inhibition of cell proliferation in Saccharomyces cerevisiae by expression of human NAD+ ADP-ribosyltransferase requires the DNA binding domain ("zinc fingers").

Authors: P Kaiser; B Auer; M Schweiger
Journal: Mol Gen Genet Date: 1992-03

10. Human nuclear NAD+ ADP-ribosyltransferase: localization of the gene on chromosome 1q41-q42 and expression of an active human enzyme in Escherichia coli.

Authors: H Herzog; B U Zabel; R Schneider; B Auer; M Hirsch-Kauffmann; M Schweiger
Journal: Proc Natl Acad Sci U S A Date: 1989-05 Impact factor: 11.205

Isolation of ADP-ribosyltransferase by affinity chromatography.

1. No abnormalities in the NAD+ ADP-ribosyltransferase (polymerizing) gene of transformed cells from a Fanconi's anemia patient.

2. Binding of NAD+ by cholera toxin.

3. ADP-ribosyltransferase from Helix pomatia. Purification and characterization.

4. Inhibition of cell proliferation in Saccharomyces cerevisiae by expression of human NAD+ ADP-ribosyltransferase requires the DNA binding domain ("zinc fingers").

5. Inhibition of poly(ADP-ribose) formation by 4-hydroxynonenal in primary cultures of rabbit synovial fibroblasts.

6. Purification and characterization of NAD+:ADP-ribosyltransferase (polymerizing) from Dictyostelium discoideum.

7. Transcriptional regulation and autoregulation of the human gene for ADP-ribosyltransferase.

Review 8. Poly(ADP-ribose) polymerase: structural conservation among different classes of animals and its implications.

9. Purification and cDNA cloning of maize Poly(ADP)-ribose polymerase.

10. Human nuclear NAD+ ADP-ribosyltransferase: localization of the gene on chromosome 1q41-q42 and expression of an active human enzyme in Escherichia coli.