T M Morgan1,2, X Wang3,4, X Qian3,5, J M Switchenko3,6, S Nie3,5, K R Patel7, R J Cassidy8,3, D M Shin3,4, J J Beitler8,3. 1. Department of Radiation Oncology, Winship Cancer Institute, Emory University School of Medicine, 1365 Clifton Road NE, Atlanta, GA, 30322, USA. tiffany.morgan2@emory.edu. 2. Winship Cancer Institute, Emory University, Atlanta, GA, USA. tiffany.morgan2@emory.edu. 3. Winship Cancer Institute, Emory University, Atlanta, GA, USA. 4. Department of Hematology and Medical Oncology, Emory University, Atlanta, GA, USA. 5. Department of Biomedical Engineering and Chemistry, Emory University, Atlanta, GA, USA. 6. Department of Biostatistics and Bioinformatics, Emory University, Atlanta, GA, USA. 7. Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, CT, USA. 8. Department of Radiation Oncology, Winship Cancer Institute, Emory University School of Medicine, 1365 Clifton Road NE, Atlanta, GA, 30322, USA.
Abstract
OBJECTIVES: We report the outcomes of patients with squamous cell carcinoma of the head and neck (HNSCC) whose circulating tumor cells (CTCs) were quantified using surface-enhanced Raman scattering (SERS) nanotechnology. METHODS: SERS tagged with EGF was used to directly measure targeted CTCs. Patient charts were retrospectively reviewed. An optimal cut point for CTCs in 7.5 ml of peripheral blood predictive of for distant metastasis-free survival (DMFS) was identified by maximizing the log-rank statistic. An ROC analysis was also performed. RESULTS: Of 82 patients, 13 experienced metastatic progression. The optimal cut point for DMFS was 675 CTCs (p = 0.047). For those with distant recurrence (n = 13) versus those without distant recurrence (n = 69), the CTC cut point which results in the largest combined sensitivity and specificity values is also 675 (sensitivity = 69%, specificity = 68%). CONCLUSION: Liquid biopsy techniques in HNSCC show promise as a means of identifying patients at greater risk of disease progression.
OBJECTIVES: We report the outcomes of patients with squamous cell carcinoma of the head and neck (HNSCC) whose circulating tumor cells (CTCs) were quantified using surface-enhanced Raman scattering (SERS) nanotechnology. METHODS:SERS tagged with EGF was used to directly measure targeted CTCs. Patient charts were retrospectively reviewed. An optimal cut point for CTCs in 7.5 ml of peripheral blood predictive of for distant metastasis-free survival (DMFS) was identified by maximizing the log-rank statistic. An ROC analysis was also performed. RESULTS: Of 82 patients, 13 experienced metastatic progression. The optimal cut point for DMFS was 675 CTCs (p = 0.047). For those with distant recurrence (n = 13) versus those without distant recurrence (n = 69), the CTC cut point which results in the largest combined sensitivity and specificity values is also 675 (sensitivity = 69%, specificity = 68%). CONCLUSION: Liquid biopsy techniques in HNSCC show promise as a means of identifying patients at greater risk of disease progression.
Entities:
Keywords:
Carcinoma, squamous cell of head and neck; Neoplasm metastasis; Neoplastic cells, circulating; Precision medicine; Prognosis
Authors: K Kian Ang; Jonathan Harris; Richard Wheeler; Randal Weber; David I Rosenthal; Phuc Felix Nguyen-Tân; William H Westra; Christine H Chung; Richard C Jordan; Charles Lu; Harold Kim; Rita Axelrod; C Craig Silverman; Kevin P Redmond; Maura L Gillison Journal: N Engl J Med Date: 2010-06-07 Impact factor: 91.245
Authors: Shyamala Maheswaran; Lecia V Sequist; Sunitha Nagrath; Lindsey Ulkus; Brian Brannigan; Chey V Collura; Elizabeth Inserra; Sven Diederichs; A John Iafrate; Daphne W Bell; Subba Digumarthy; Alona Muzikansky; Daniel Irimia; Jeffrey Settleman; Ronald G Tompkins; Thomas J Lynch; Mehmet Toner; Daniel A Haber Journal: N Engl J Med Date: 2008-07-02 Impact factor: 91.245