| Literature DB >> 30083841 |
Rong-Shing Chang1, Chi-Shuo Chen1, Ching-Lung Huang1, Chiu-Ting Chang1, Yujia Cui1, Wei-Ju Chung, Wun-Yi Shu2, Chi-Shiun Chiang1, Chun-Yu Chuang1, Ian C Hsu3.
Abstract
Application of high-dosage UVB irradiation in phototherapeutic dermatological treatments present health concerns attributed to UV-exposure. In assessing UV-induced photobiological damage, we investigated dose-dependent effects of UVB irradiation on human keratinocyte cells (HaCaT). Our study implemented survival and apoptosis assays and revealed an unexpected dose response wherein higher UVB-dosage induced higher viability. Established inhibitors, such as AKT- (LY294002), PKC- (Gö6976, and Rottlerin), ERK- (PD98059), P38 MAPK- (SB203580), and JNK- (SP600125), were assessed to investigate UV-induced apoptotic pathways. Despite unobvious contributions of known signaling pathways in dose-response mediation, microarray analysis identified transcriptional expression of UVB-response genes related to the respiratory-chain. Observed correlation of ROS-production with UVB irradiation potentiated ROS as the underlying mechanism for observed dose responses. Inability of established pathways to explain such responses suggests the complex nature underlying UVB-phototherapy response.Entities:
Keywords: Apoptosis; Phototherapy; Proliferation; ROS, reactive oxygen species; UVB, ultraviolet B
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Year: 2018 PMID： 30083841 DOI： 10.1007/s11626-018-0280-4
Source DB: PubMed Journal: In Vitro Cell Dev Biol Anim ISSN： 1071-2690 Impact factor: 2.416