Literature DB >> 30083673

A coupled chlorinase-fluorinase system with a high efficiency of trans-halogenation and a shared substrate tolerance.

H Sun1, H Zhao, E L Ang.   

Abstract

Enzymatic trans-halogenation enables radiolabeling under mild and aqueous conditions, but rapid reactions are desired. We developed a coupled chlorinase-fluorinase system for rapid trans-halogenation. Notably, the chlorinase shares a substrate tolerance with the fluorinase, enabling these two enzymes to cooperatively produce 5'-fluorodeoxy-2-ethynyladenosine (5'-FDEA) in up to 91.6% yield in 1 h.

Entities:  

Year:  2018        PMID： 30083673      PMCID： PMC6113055          DOI： 10.1039/c8cc04436h

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  23 in total

Review 1.  Specialised metabolites regulating antibiotic biosynthesis in Streptomyces spp.

Authors:  Guoqing Niu; Keith F Chater; Yuqing Tian; Jihui Zhang; Huarong Tan
Journal:  FEMS Microbiol Rev       Date:  2016-06-09       Impact factor: 16.408

2.  Mechanism of enzymatic fluorination in Streptomyces cattleya.

Authors:  Xiaofeng Zhu; David A Robinson; Andrew R McEwan; David O'Hagan; James H Naismith
Journal:  J Am Chem Soc       Date:  2007-11-07       Impact factor: 15.419

3.  Isolation and characterisation of 5'-fluorodeoxyadenosine synthase, a fluorination enzyme from Streptomyces cattleya.

Authors:  Christoph Schaffrath; Hai Deng; David O'Hagan
Journal:  FEBS Lett       Date:  2003-07-17       Impact factor: 4.124

4.  Identification of genetic variations associated with epsilon-poly-lysine biosynthesis in Streptomyces albulus ZPM by genome sequencing.

Authors:  Lin Wang; Chunhui Gao; Nan Tang; Songnian Hu; Qingfa Wu
Journal:  Sci Rep       Date:  2015-03-17       Impact factor: 4.379

Review 5.  Clinical Application of Radiolabeled RGD Peptides for PET Imaging of Integrin αvβ3.

Authors:  Haojun Chen; Gang Niu; Hua Wu; Xiaoyuan Chen
Journal:  Theranostics       Date:  2016-01-01       Impact factor: 11.556

6.  antiSMASH 3.0-a comprehensive resource for the genome mining of biosynthetic gene clusters.

Authors:  Tilmann Weber; Kai Blin; Srikanth Duddela; Daniel Krug; Hyun Uk Kim; Robert Bruccoleri; Sang Yup Lee; Michael A Fischbach; Rolf Müller; Wolfgang Wohlleben; Rainer Breitling; Eriko Takano; Marnix H Medema
Journal:  Nucleic Acids Res       Date:  2015-05-06       Impact factor: 16.971

7.  A roadmap for natural product discovery based on large-scale genomics and metabolomics.

Authors:  James R Doroghazi; Jessica C Albright; Anthony W Goering; Kou-San Ju; Robert R Haines; Konstantin A Tchalukov; David P Labeda; Neil L Kelleher; William W Metcalf
Journal:  Nat Chem Biol       Date:  2014-09-28       Impact factor: 15.040

8.  Discovery and characterization of a marine bacterial SAM-dependent chlorinase.

Authors:  Alessandra S Eustáquio; Florence Pojer; Joseph P Noel; Bradley S Moore
Journal:  Nat Chem Biol       Date:  2007-12-02       Impact factor: 15.040

9.  Identification of fluorinases from Streptomyces sp MA37, Norcardia brasiliensis, and Actinoplanes sp N902-109 by genome mining.

Authors:  Hai Deng; Long Ma; Nouchali Bandaranayaka; Zhiwei Qin; Greg Mann; Kwaku Kyeremeh; Yi Yu; Thomas Shepherd; James H Naismith; David O'Hagan
Journal:  Chembiochem       Date:  2014-01-21       Impact factor: 3.164

10.  A localized tolerance in the substrate specificity of the fluorinase enzyme enables "last-step" 18F fluorination of a RGD peptide under ambient aqueous conditions.

Authors:  Stephen Thompson; Qingzhi Zhang; Mayca Onega; Stephen McMahon; Ian Fleming; Sharon Ashworth; James H Naismith; Jan Passchier; David O'Hagan
Journal:  Angew Chem Int Ed Engl       Date:  2014-07-02       Impact factor: 15.336
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  4 in total

1.  A Nonconventional Archaeal Fluorinase Identified by In Silico Mining for Enhanced Fluorine Biocatalysis.

Authors:  Isabel Pardo; David Bednar; Patricia Calero; Daniel C Volke; Jiří Damborský; Pablo I Nikel
Journal:  ACS Catal       Date:  2022-05-19       Impact factor: 13.700

2.  Oligomerization engineering of the fluorinase enzyme leads to an active trimer that supports synthesis of fluorometabolites in vitro.

Authors:  Tiia Kittilä; Patricia Calero; Folmer Fredslund; Phillip T Lowe; David Tezé; Manuel Nieto-Domínguez; David O'Hagan; Pablo I Nikel; Ditte H Welner
Journal:  Microb Biotechnol       Date:  2022-01-27       Impact factor: 6.575

Review 3.  Halogenating Enzymes for Active Agent Synthesis: First Steps Are Done and Many Have to Follow.

Authors:  Alexander Veljko Fejzagić; Jan Gebauer; Nikolai Huwa; Thomas Classen
Journal:  Molecules       Date:  2019-11-05       Impact factor: 4.411

4.  Whole-cell catalysis by surface display of fluorinase on Escherichia coli using N-terminal domain of ice nucleation protein.

Authors:  Xinming Feng; Miaomiao Jin; Wei Huang; Wei Liu; Mo Xian
Journal:  Microb Cell Fact       Date:  2021-10-29       Impact factor: 5.328
  4 in total

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