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Literature DB >> 3008090

Cloning and expression of cDNA encoding mouse tyrosinase.

S Shibahara, Y Tomita, T Sakakura, C Nager, B Chaudhuri, R Müller.

Abstract

We have isolated a pigment cell-specific cDNA clone from a B16 mouse melanoma cDNA library by differential hybridization. The mRNA of isolated cDNA is highly expressed in B16 melanoma cells and in black mouse (C57BL/6) skin, but is not detectable in mouse neuroblastoma cells nor in K1735 mouse amelanotic melanoma cells. The protein sequence deduced from the nucleotide sequence of the cloned cDNA shows significant similarity to the entire region of Neurospora tyrosinase. To know the identity of cDNA, we transfected K1735 amelanotic melanoma and COS-7 cells with the cDNA carried in a simian virus 40 vector (pKCRH2). We confirmed that the isolated cDNA encodes mouse tyrosinase by immunofluorescence staining of transfected cells using two different anti-T4-tyrosinase monoclonal antibodies. Tyrosinase is composed of 513 amino acids with a molecular weight of 57,872 excluding a hydrophobic signal peptide of 24 amino acids.

Entities: Disease Gene Species

Mesh：

Substances：

Year: 1986 PMID： 3008090 PMCID： PMC339673 DOI： 10.1093/nar/14.6.2413

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

42 in total

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49 in total

1. Genetic, cytogenetic, and molecular analyses of mutations induced by melphalan demonstrate high frequencies of heritable deletions and other rearrangements from exposure of postspermatogonial stages of the mouse.

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Journal: Proc Natl Acad Sci U S A Date: 1992-07-01 Impact factor: 11.205

2. Nucleotide sequence of the cDNA encoding human tyrosinase-related protein.

Authors: T Cohen; R M Muller; Y Tomita; S Shibahara
Journal: Nucleic Acids Res Date: 1990-05-11 Impact factor: 16.971

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Journal: Proc Natl Acad Sci U S A Date: 1987-11 Impact factor: 11.205

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Journal: Biochem J Date: 1989-08-15 Impact factor: 3.857

5. A two allele XbaI RFLP at the catalase 2 locus.

Authors: M A Colman; S Shibahara; B Kwon; T Jenkins; M Ramsay
Journal: Nucleic Acids Res Date: 1991-02-25 Impact factor: 16.971

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Authors: C D Chintamaneni; R Halaban; Y Kobayashi; C J Witkop; B S Kwon
Journal: Proc Natl Acad Sci U S A Date: 1991-06-15 Impact factor: 11.205

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Authors: R E Boissy; H Zhao; W S Oetting; L M Austin; S C Wildenberg; Y L Boissy; Y Zhao; R A Sturm; V J Hearing; R A King; J J Nordlund
Journal: Am J Hum Genet Date: 1996-06 Impact factor: 11.025

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Authors: A Urquhart
Journal: Nucleic Acids Res Date: 1991-10-25 Impact factor: 16.971

9. Genetic and molecular analysis of chlorambucil-induced germ-line mutations in the mouse.

Authors: E M Rinchik; J W Bangham; P R Hunsicker; N L Cacheiro; B S Kwon; I J Jackson; L B Russell
Journal: Proc Natl Acad Sci U S A Date: 1990-02 Impact factor: 11.205

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Authors: K Yasumoto; K Yokoyama; K Shibata; Y Tomita; S Shibahara
Journal: Mol Cell Biol Date: 1994-12 Impact factor: 4.272