| Literature DB >> 30080672 |
Hong-Liang Zhang1, Yan-Dong Tang1, Chun-Xiao Liu1, Li-Run Xiang1, Wen-Li Zhang1, Chao-Liang Leng2, Qian Wang1, Tong-Qing An1, Jin-Mei Peng1, Zhi-Jun Tian3, Xue-Hui Cai4.
Abstract
The recent rapid evolution of PRRSVs has resulted in certain biological characteristic changes, such as the fact that an increasing number of field PRRSVs can be isolated from PAMs but not from Marc-145 cells. In this study, we first isolated Marc-145-unadaptive field PRRSV strains from PAMs; sequence analysis showed that these PRRSVs belong to the HP-PRRSV (lineage 8) branch or NADC30-Like (lineage 1) branch. We further found major variations in ORF2-4 regions. To explore the viral adaptation mechanisms in detail, we constructed a full-length cDNA clone of MY-376, a Marc-145-unadaptive PRRSV. Construction of serially chimeric viruses of HuN4-F112 (a Marc-145-adaptive strain) and MY-376 demonstrated that variation in the minor envelope protein (GP2a and GP3) complex is a main determinant of PRRSV tropism for Marc-145 cells.Entities:
Keywords: GP2a and GP3; MY-376; Marc-145-unadaptive; Tropism; Variation
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Year: 2018 PMID： 30080672 DOI： 10.1016/j.vetmic.2018.06.021
Source DB: PubMed Journal: Vet Microbiol ISSN： 0378-1135 Impact factor: 3.293