Use of biogas digestates obtained by anaerobic digestion and co-digestion as fertilizers: Characterization, soil biological activity and growth dynamic of Lactuca sativa L.

Agro-industrial systems provide large quantities of organic wastes that could imply an important environmental risk. While manures can be easily treated by anaerobic digestion, horticultural fruit wastes generally cannot be processed alone and should be treated by co-digestion. To use organic wastes as fertilizers is fundamental to improve understanding of their impact on soil-plant systems. In this research, cattle manure, poultry litter, pig slurry and onion waste were collected. Animal manures were studied without treatment, treated by anaerobic digestion alone and in co-digestion with onion wastes. To study their effect on soil-plant systems, chemical and spectroscopic characterization of manures and their transformed products were combined with soil biological activity and growth dynamic of lettuce following wastes incorporation to the soil. Anaerobic digestion decreased the C/N ratio, whilst there was an increase in NH4+-N/N ratio and short-chain organic acids. The magnitude of these changes varied depending on the type of organic matter present in each material and the incorporation of onion wastes intensified them. However, the digestates presented similar structural characteristics to each other, independently of the material of origin. Digestate soil application produced a fast and short microbial stimulation (18-34 and 7-11 mg CO2 during the first 6 h, digestates vs. rest of treatments). The digestate dosage should be done according to the content of NH4+-N given that the vegetal growth is related to it. Soils amended with digestates showed less CO2 emission than soils amended with manures improving overall C balance.