Literature DB >> 3007449

Effect of trypsin on the kinetic properties of reconstituted beef heart cytochrome c oxidase.

U Büge, B Kadenbach.   

Abstract

Isolated beef heart cytochrome c oxidase was reconstituted in liposomes by the cholate dialysis method with 85% of the binding site for cytochrome c oriented to the outside. Trypsin cleaved specifically subunit VIa and half of subunit IV from the reconstituted enzyme. The kinetic properties of the reconstituted enzyme were changed by trypsin treatment if measured by the spectrophotometric assay but not by the polarographic assay. It is concluded that subunit VIa and/or subunit IV participate in the electron transport activity of cytochrome c oxidase.

Entities:  

Mesh：

Substances：

Year:  1985        PMID： 3007449     DOI： 10.1007/bf00743110

Source DB:  PubMed          Journal:  J Bioenerg Biomembr        ISSN： 0145-479X            Impact factor:   2.945


  26 in total

1.  Correlation of the kinetics of electron transfer activity of various eukaryotic cytochromes c with binding to mitochondrial cytochrome c oxidase.

Authors:  S Ferguson-Miller; D L Brautigan; E Margoliash
Journal:  J Biol Chem       Date:  1976-02-25       Impact factor: 5.157

2.  Structure of the cytochrome c oxidase complex of rat liver. 2. Topological orientation of polypeptides in the membrane as studied by proteolytic digestion and immunoblotting.

Authors:  J Jarausch; B Kadenbach
Journal:  Eur J Biochem       Date:  1985-01-02

3.  Immunological identification of four different polypeptides in 'subunit VII' of mammalian cytochrome c oxidase.

Authors:  L Kuhn-Nentwig; B Kadenbach
Journal:  FEBS Lett       Date:  1984-07-09       Impact factor: 4.124

4.  Tissue-specificity overrides species-specificity in cytoplasmic cytochrome c oxidase polypeptides.

Authors:  J Jarausch; B Kadenbach
Journal:  Hoppe Seylers Z Physiol Chem       Date:  1982-09

5.  Limited-turnover studies on proton translocation in reconstituted cytochrome c oxidase-containing vesicles.

Authors:  R P Casey; J B Chappell; A Azzi
Journal:  Biochem J       Date:  1979-07-15       Impact factor: 3.857

6.  The subunit composition of mammalian cytochrome c oxidase.

Authors:  P Merle; B Kadenbach
Journal:  Eur J Biochem       Date:  1980-04

7.  Different reactivity of carboxylic groups of cytochrome c oxidase polypeptides from pig liver and heart.

Authors:  B Kadenbach; A Stroh
Journal:  FEBS Lett       Date:  1984-08-06       Impact factor: 4.124

8.  Binding of arylazidocytochrome c derivatives to beef heart cytochrome c oxidase: cross-linking in the high- and low-affinity binding sites.

Authors:  R Bisson; B Jacobs; R A Capaldi
Journal:  Biochemistry       Date:  1980-09-02       Impact factor: 3.162

9.  Topology of beef heart cytochrome c oxidase from studies on reconstituted membranes.

Authors:  Y Z Zhang; G Georgevich; R A Capaldi
Journal:  Biochemistry       Date:  1984-11-06       Impact factor: 3.162

10.  Immunological and chemical characterization of rat liver cytochrome c oxidase.

Authors:  P Merle; J Jarausch; M Trapp; R Scherka; B Kadenbach
Journal:  Biochim Biophys Acta       Date:  1981-07-28
View more
  2 in total

Review 1.  Regulation of respiration and ATP synthesis in higher organisms: hypothesis.

Authors:  B Kadenbach
Journal:  J Bioenerg Biomembr       Date:  1986-02       Impact factor: 2.945

2.  Sequential dissociation of subunits from bovine heart cytochrome C oxidase by urea.

Authors:  Erik Sedlák; Neal C Robinson
Journal:  Biochemistry       Date:  2009-09-01       Impact factor: 3.162
  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.