Muthukumar Thangavelu1, Aravinthan Adithan2, Sastry Thotapalli Parvathaleswara3, Chamundeeswari Munusamy4. 1. Department of Ophthalmology, Institute for Clinical and Experimental Medicine, Linkoping University, 581 83, Linkoping, Sweden. 2. Department of Physiology, College of Veterinary Medicine, Chonbuk National University, 79 Gobong-ro, Iksan-city, Jeollabuk-Do, 570-752, Republic of Korea. 3. Bio-Products Laboratory, CSIR-Central Leather Research Institute, Adyar, Chennai, Tamilnadu, 600020, India. 4. Department of Biotechnology, St. Joseph's College of Engineering, Sholinganallur, Chennai, 600 119, India. chamundeeswari@gmail.com.
Abstract
PURPOSE: Production of highly penetrable and targetable drug delivery particles is mainly focused by current therapy and such focus is achieved in our present study. The carbon nanoparticle (CNP) prepared from purely natural source was modified from spherical shape to cylindrical floral like structure after treatment with the anticancer drug methotrexate (CM). METHODS: The physiochemical properties of the CNP and CM was characterized using FT-IR/Raman Spectrometer, XRD, SEM, AFM, particle size analyzer and its biological evaluation using haemolysis and MTT assay. RESULTS: The shift in FT-IR peaks at 1592, 1120 cm-1 and peaks of raman spectra observed at 1303, 1300 cm-1 represents ordered carbon nanotubes. The morphological change from spherical to cylindrical floral like structure was observed using SEM and AFM and its particle size distribution analysis shows an average diameter of 269 nm for CM. XRD peak at 2θ = 23.86° (002) indicates the presence of large amount of amorphous material that corresponds to multi-walled carbon nanotubes. Haemocompatibility studies proved the safety level usage as 100 μg/ml and MTT assay shows viability rate of 85-98% with mouse embryonic fibroblast (NIH/3 T3) and 30-45% with pancreatic carcinoma (MIA PaCa-2) and gastric cancer cell lines (SNU- 484) respectively.These results are also supported by phase contrast microscope images observed after staining with calcein AM and EthD-1. CONCLUSIONS: The morphologically modified CNPs has shown good anticancer, biocompatibility and haemocompatibility property which is an important criterion to be satisfied by a biomedical product.
PURPOSE: Production of highly penetrable and targetable drug delivery particles is mainly focused by current therapy and such focus is achieved in our present study. The carbon nanoparticle (CNP) prepared from purely natural source was modified from spherical shape to cylindrical floral like structure after treatment with the anticancer drug methotrexate (CM). METHODS: The physiochemical properties of the CNP and CM was characterized using FT-IR/Raman Spectrometer, XRD, SEM, AFM, particle size analyzer and its biological evaluation using haemolysis and MTT assay. RESULTS: The shift in FT-IR peaks at 1592, 1120 cm-1 and peaks of raman spectra observed at 1303, 1300 cm-1 represents ordered carbon nanotubes. The morphological change from spherical to cylindrical floral like structure was observed using SEM and AFM and its particle size distribution analysis shows an average diameter of 269 nm for CM. XRD peak at 2θ = 23.86° (002) indicates the presence of large amount of amorphous material that corresponds to multi-walled carbon nanotubes. Haemocompatibility studies proved the safety level usage as 100 μg/ml and MTT assay shows viability rate of 85-98% with mouse embryonic fibroblast (NIH/3 T3) and 30-45% with pancreatic carcinoma (MIA PaCa-2) and gastric cancer cell lines (SNU- 484) respectively.These results are also supported by phase contrast microscope images observed after staining with calcein AM and EthD-1. CONCLUSIONS: The morphologically modified CNPs has shown good anticancer, biocompatibility and haemocompatibility property which is an important criterion to be satisfied by a biomedical product.
Authors: Stephanie E A Gratton; Patricia A Ropp; Patrick D Pohlhaus; J Christopher Luft; Victoria J Madden; Mary E Napier; Joseph M DeSimone Journal: Proc Natl Acad Sci U S A Date: 2008-08-12 Impact factor: 11.205