L Zhang1, D-L Wang, P Yu. 1. Department of Obstetrics, Dezhou People's Hospital, Dezhou, China. yu.acool@163.com.
Abstract
OBJECTIVE: The role of long non-coding RNA (lncRNA) H19 in endometrial carcinoma was studied, and its mechanism was also explored. PATIENTS AND METHODS: Quantitative Reverse Transcriptase-Polymerase Chain Reaction (qRT-PCR) was used to detect the expression of lncRNA H19, miR-612, and HOXA10 in endometrial carcinoma, and the relationship between lncRNA H19 and survival time was analyzed. The high expression or knockdown of lncRNA H19 in endometrial cancer cells was completed by cell transfection experiments. Cell counting kit-8 (CCK-8) assay was used to detect changes in the viability of endometrial cancer cells. Dual luciferase reporter assay was performed to verify that miR-612 could bind to lncRNA H19 or HOXA10. QRT-PCR and Western Blot assays were used to detect changes in the expression of HOXA10 in endometrial cancer cells before and after overexpression or knockdown of lncRNA H19. RESULTS: The expression of lncRNA H19 and HOXA10 was high in endometrial carcinoma and miR-612 was lowly expressed. The survival curve suggested that lncRNA H19 was negatively correlated with patient survival. The mRNA expression of lncRNA H19 in endometrial cancer cells, including HEC1-A, HEC1-B, AN3CA, and Ishikawa, was detected by qRT-PCR. It was found that the expression of lncRNA H19 was highest in AN3CA and lowest in Ishikawa. The cell transfection experiments allowed Ishikawa cells to overexpress lncRNA H19 and AN3CA cells to reduce lncRNA H19 expression. After overexpression of lncRNA H19, the viability of Ishikawa cells as well as the mRNA and protein levels of HOXA10 increased. However, after knocking down lncRNA H19, the viability of AN3CA cells along with the mRNA and protein levels of HOXA10 decreased. The dual luciferase reporter assay results suggested that miR-612 could bind to lncRNA H19 and HOXA10. CONCLUSIONS: The high expression of lncRNA H19 in endometrial carcinoma may regulate the expression level of its target gene HOXA10 by targeting miR-612, thus promoting cell proliferation to play a role in the development of endometrial carcinoma.
OBJECTIVE: The role of long non-coding RNA (lncRNA) H19 in endometrial carcinoma was studied, and its mechanism was also explored. PATIENTS AND METHODS: Quantitative Reverse Transcriptase-Polymerase Chain Reaction (qRT-PCR) was used to detect the expression of lncRNA H19, miR-612, and HOXA10 in endometrial carcinoma, and the relationship between lncRNA H19 and survival time was analyzed. The high expression or knockdown of lncRNA H19 in endometrial cancer cells was completed by cell transfection experiments. Cell counting kit-8 (CCK-8) assay was used to detect changes in the viability of endometrial cancer cells. Dual luciferase reporter assay was performed to verify that miR-612 could bind to lncRNA H19 or HOXA10. QRT-PCR and Western Blot assays were used to detect changes in the expression of HOXA10 in endometrial cancer cells before and after overexpression or knockdown of lncRNA H19. RESULTS: The expression of lncRNA H19 and HOXA10 was high in endometrial carcinoma and miR-612 was lowly expressed. The survival curve suggested that lncRNA H19 was negatively correlated with patient survival. The mRNA expression of lncRNA H19 in endometrial cancer cells, including HEC1-A, HEC1-B, AN3CA, and Ishikawa, was detected by qRT-PCR. It was found that the expression of lncRNA H19 was highest in AN3CA and lowest in Ishikawa. The cell transfection experiments allowed Ishikawa cells to overexpress lncRNA H19 and AN3CA cells to reduce lncRNA H19 expression. After overexpression of lncRNA H19, the viability of Ishikawa cells as well as the mRNA and protein levels of HOXA10 increased. However, after knocking down lncRNA H19, the viability of AN3CA cells along with the mRNA and protein levels of HOXA10 decreased. The dual luciferase reporter assay results suggested that miR-612 could bind to lncRNA H19 and HOXA10. CONCLUSIONS: The high expression of lncRNA H19 in endometrial carcinoma may regulate the expression level of its target gene HOXA10 by targeting miR-612, thus promoting cell proliferation to play a role in the development of endometrial carcinoma.
Authors: Daniella P Vansteenkiste; Joelle M Fenger; Paolo Fadda; Paula Martin-Vaquero; Ronaldo C da Costa Journal: J Vet Intern Med Date: 2019-10-22 Impact factor: 3.333