Guang-Yong Ye1, Ke-Yi Wang2, Qiao-di Gui3, Min Wang1. 1. Women's Hospital, Zhejiang University School of Medicine, Hangzhou 310006, China. 2. Central Laboratory, Hangzhou First People's Hospital, Hangzhou 310006, China. 3. Department of Clinical Laboratory, Shaanxi Provincial People's Hospital, Xi'an 710068, China.
Abstract
OBJECTIVE: The purpose of this study was to determine the role of Ureaplasma urealyticum-derived lipid-associated membrane proteins (LAMPs) in the host innate immune system, specifically their effect on Toll-like receptors (TLRs). METHODS: LAMPs were derived from U. urealyticum strains, and human amniotic epithelial cells (HAECs) were isolated from healthy full-term placentas. Cytokine concentrations were determined by enzyme-linked immunosorbent assay (ELISA) and TLR2 mRNA by real-time PCR. Expression of TLR2 was confirmed by Western blotting and immunohistochemistry. RESULTS: LAMPs induced HAECs to produce inflammatory cytokines interleukin (IL)-6, IL-8, and tumor necrosis factor (TNF)-α. Cytokine production was reduced after blocking TLR2 using TLR2 inhibitor (anti-hTLR2-IgA). CONCLUSIONS: LAMPs isolated from U. urealyticum induced TLR2-dependent up-regulation of inflammatory genes and cytokines in HAECs.
OBJECTIVE: The purpose of this study was to determine the role of Ureaplasma urealyticum-derived lipid-associated membrane proteins (LAMPs) in the host innate immune system, specifically their effect on Toll-like receptors (TLRs). METHODS: LAMPs were derived from U. urealyticum strains, and human amniotic epithelial cells (HAECs) were isolated from healthy full-term placentas. Cytokine concentrations were determined by enzyme-linked immunosorbent assay (ELISA) and TLR2 mRNA by real-time PCR. Expression of TLR2 was confirmed by Western blotting and immunohistochemistry. RESULTS: LAMPs induced HAECs to produce inflammatory cytokines interleukin (IL)-6, IL-8, and tumor necrosis factor (TNF)-α. Cytokine production was reduced after blocking TLR2 using TLR2 inhibitor (anti-hTLR2-IgA). CONCLUSIONS: LAMPs isolated from U. urealyticum induced TLR2-dependent up-regulation of inflammatory genes and cytokines in HAECs.
Authors: Olga V Glushkova; Svetlana B Parfenyuk; Maxim O Khrenov; Tatyana V Novoselova; Sergey M Lunin; Eugeny E Fesenko; Elena G Novoselova Journal: J Immunotoxicol Date: 2012-07-25 Impact factor: 3.000
Authors: Eui Jeong Noh; Dong Jae Kim; Jun Young Lee; Jong Hwan Park; Jong-Seok Kim; Jae Won Han; Byoung Chan Kim; Chul Jung Kim; Sung Ki Lee Journal: Front Immunol Date: 2019-10-04 Impact factor: 7.561