| Literature DB >> 30068645 |
Ting Pan1,2,3, Zhilin Peng1,2,3, Likai Tan1,2,3, Fan Zou1,2,3, Nan Zhou1,2,3, Bingfeng Liu1,2,3, Liting Liang1,2,3, Cancan Chen1,2,3, Jun Liu1,2,3, Liyang Wu1,2,3, Guangyan Liu1,2,3, Zhiqin Peng1,2,3, Weiwei Liu1,2,3, Xiancai Ma1,2,3, Junsong Zhang1,2,3, Xun Zhu2,4, Ting Liu1,2,5, Mengfeng Li2,4, Xi Huang1,2,5, Liang Tao6, Yiwen Zhang7,2,3, Hui Zhang7,2,3,4.
Abstract
Zika virus (ZIKV) is genetically and biologically related to other Flaviviridae family members and has disseminated to many countries. It is associated with severe consequences, including the abnormal development of the neural system in fetuses and neurological diseases in adults. Therefore, the development of anti-ZIKV drugs is of paramount importance. Screening of generic drugs revealed that several nonsteroidal anti-inflammatory drugs (NSAIDs), including aspirin, ibuprofen, naproxen, acetaminophen, and lornoxicam, potently inhibited the entry of Zika virus Env/HIV-1-pseudotyped viruses. They also significantly inhibited the replication of wild-type ZIKV both in cell lines and in primary human fetal endothelial cells. Interestingly, the NSAIDs exerted this inhibitory effect by potently reducing the expression of AXL, the entry cofactor of ZIKV. Further studies showed that the NSAIDs downregulated the prostaglandin E2/prostaglandin E receptor 2 (EP2)/cAMP/protein kinase A (PKA) signaling pathway and reduced PKA-dependent CDC37 phosphorylation and the interaction between CDC37 and HSP90, which subsequently facilitated CHIP/ubiquitination/proteasome-mediated AXL degradation. Taken together, our results highlight a new mechanism of action of antiviral agents which may assist in designing a convenient strategy for treating ZIKV-infected patients.IMPORTANCE Zika virus (ZIKV) infection, which causes congenital malformations, including microcephaly and other neurological disorders, has attracted global attention. We observed that several NSAIDs significantly inhibited ZIKV infection. Based on our observations, we propose a novel mechanism of action of antiviral compounds which involves the blockade of virus entry via degradation of the entry cofactor. Furthermore, NSAIDs can be practically used for preventing ZIKV infection in pregnant women, as certain NSAIDs, including ibuprofen and acetaminophen, are considered clinically safe.Entities:
Keywords: AXL; NSAID; ZIKV; ubiquitination
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Year: 2018 PMID： 30068645 PMCID： PMC6158411 DOI： 10.1128/JVI.01018-18
Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103