Sarah R Martha1, Lisa A Collier2, Stephanie M Davis2, Hilary A Seifert3, Christopher C Leonardo4, Craig T Ajmo4, Elspeth A Foran4, Justin F Fraser5, Keith R Pennypacker6. 1. College of Nursing, University of Kentucky, Lexington, Kentucky. Electronic address: sarah.martha@uky.edu. 2. Department of Neurology, College of Medicine, University of Kentucky, Lexington, Kentucky. 3. Department of Neurology, School of Medicine, Oregon Health & Science University, Portland, Oregon. 4. Department of Molecular Pharmacology and Physiology, Morsani College of Medicine, University of Southern Florida, Tampa, Florida. 5. Department of Neurology, College of Medicine, University of Kentucky, Lexington, Kentucky; Department of Neurosurgery, College of Medicine, University of Kentucky, Lexington, Kentucky; Department of Neuroscience, College of Medicine, University of Kentucky, Lexington, Kentucky; Department of Radiology, College of Medicine, University of Kentucky, Lexington, Kentucky. 6. Department of Neurology, College of Medicine, University of Kentucky, Lexington, Kentucky; Department of Neuroscience, College of Medicine, University of Kentucky, Lexington, Kentucky.
Abstract
BACKGROUND AND PURPOSE: Acid/base and electrolytes could provide clinically valuable information about cerebral infarct core and penumbra. We evaluated associations between acid/base and electrolyte changes and outcomes in 2 rat models of stroke, permanent, and transient middle cerebral artery occlusion. METHODS: Three-month old Sprague-Dawley rats underwent permanent or transient middle cerebral artery occlusion. Pre- and post-middle cerebral artery occlusion venous samples for permanent and transient models provided pH, carbon dioxide, oxygen, glucose, and electrolyte values of ionized calcium, potassium, and sodium. Multiple regression determined predictors of infarct volume from these values, and Kaplan-Meier curve analyzed morality between permanent and transient middle cerebral artery occlusion models. RESULTS: Analysis indicated significant differences in the blood gas and electrolytes between pre- to post-middle cerebral artery occlusion. A decrease in pH and sodium with increases in carbon dioxide, potassium, ionized calcium, and glucose changes were found in both middle cerebral artery occlusion models; while hematocrit and hemoglobin were significant in the transient model. pH and ionized calcium were predictors of infarct volume in the permanent model, as changes in pH and ionized calcium decreased, infarct volume increased. CONCLUSIONS: There are acute changes in acid/base balance and electrolytes during stroke in transient and permanent rodent models. Additionally, we found pH and ionized calcium changes predicted stroke volume in the permanent middle cerebral artery occlusion model. These preliminary findings are novel, and warrant further exploration in human conditions.
BACKGROUND AND PURPOSE: Acid/base and electrolytes could provide clinically valuable information about cerebral infarct core and penumbra. We evaluated associations between acid/base and electrolyte changes and outcomes in 2 rat models of stroke, permanent, and transient middle cerebral artery occlusion. METHODS: Three-month old Sprague-Dawley rats underwent permanent or transient middle cerebral artery occlusion. Pre- and post-middle cerebral artery occlusion venous samples for permanent and transient models provided pH, carbon dioxide, oxygen, glucose, and electrolyte values of ionizedcalcium, potassium, and sodium. Multiple regression determined predictors of infarct volume from these values, and Kaplan-Meier curve analyzed morality between permanent and transient middle cerebral artery occlusion models. RESULTS: Analysis indicated significant differences in the blood gas and electrolytes between pre- to post-middle cerebral artery occlusion. A decrease in pH and sodium with increases in carbon dioxide, potassium, ionizedcalcium, and glucose changes were found in both middle cerebral artery occlusion models; while hematocrit and hemoglobin were significant in the transient model. pH and ionizedcalcium were predictors of infarct volume in the permanent model, as changes in pH and ionizedcalcium decreased, infarct volume increased. CONCLUSIONS: There are acute changes in acid/base balance and electrolytes during stroke in transient and permanent rodent models. Additionally, we found pH and ionizedcalcium changes predicted stroke volume in the permanent middle cerebral artery occlusion model. These preliminary findings are novel, and warrant further exploration in human conditions.
Authors: Craig T Ajmo; Dionne O L Vernon; Lisa Collier; Aaron A Hall; Svitlana Garbuzova-Davis; Alison Willing; Keith R Pennypacker Journal: J Neurosci Res Date: 2008-08-01 Impact factor: 4.164
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Authors: Sarah R Martha; Lisa A Collier; Stephanie M Davis; Sarah J Goodwin; David Powell; Doug Lukins; Justin F Fraser; Keith R Pennypacker Journal: J Neurosci Res Date: 2019-04-03 Impact factor: 4.164