Photodynamic Therapy Mediated by Aloe-Emodin Inhibited Angiogenesis and Cell Metastasis Through Activating MAPK Signaling Pathway on HUVECs.

Photodynamic therapy is a clinically used, minimally invasive therapeutic procedure that involves the application of photosensitizers which can locate in target cells and so be irradiated at a corresponding wavelength. Laser light irradiation activation of photosensitizers generates free reactive oxygen species, which induces selective cytotoxic activity in target cells. Within recent years, aloe-emodin as a photosensitizer has been successfully applied in photodynamic therapy applications. Angiogenesis plays an important role in tumor growth and metastasis; thus, the development of a novel target treatment for angiogenesis is essential in order to improve treatment therapeutics for cancer treatment. An essential step in angiogenesis involves the formation of tube-like structures during matrix degradation, rearrangement, and apoptosis of endothelial cells. In the present study, we investigated the mechanisms of photocytotoxicity induced by aloe-emodin in human umbilical vein endothelial cells. Analysis of cell proliferation results noted a significant decrease in cultured cells which received various concentrations of aloe-emodin and photodynamic therapy-induced light doses. Additionally, mitochondrial mechanisms of apoptotic cell death were observed in aloe-emodin photodynamic therapy-treated cells, as tube formation assays noted angiogenesis suppression after treatment. The capacity of migration and invasion of human umbilical vein endothelial cells was measured using the transwell assay and demonstrated that aloe-emodin photodynamic therapy significantly inhibited the migration and invasion of human umbilical vein endothelial cells. The expression of p38, extracellular signal-regulated kinase, the c-Jun N-terminal kinases, and vascular endothelial growth factor suggested that the cellular metastasis was related to mitogen-activated protein kinase signal pathway. Furthermore, disorganization of F action cytoskeleton components was observed after aloe-emodin photodynamic therapy. Overall, the findings from this study suggest that aloe-emodin photodynamic therapy inhibited angiogenesis and cellular metastasis in human umbilical vein endothelial cells by activating the mitogen-activated protein kinase apoptotic signaling cell death pathway.

Introduction

Photodynamic therapy (PDT) is a new treatment that is minimally invasive and exerts selective cytotoxicity for target cells. Study showed cancers of skin, bladder, lung, esophagus, and cervix can be managed,[1] and the reactive oxygen species (ROS) will be produced when light irradiation works on photosensitizers (PSs) and induces cytotoxicity.[2] There are 3 mechanisms of PDT: (1) PDT can direct cell killing; (2) PDT can devastate the vasculature; and (3) PDT can initiate immune response. Photodynamic therapy can extend the survival and improve the quality of life for patients with inoperable cancers.[3-5] Photodynamic therapy includes 3 essential elements: PS, light, and reactive oxygen.[6,7]

Anthraquinone derivative, aloe-emodin (AE), is similar to hypericin in structure.[8,9] Previous studies had found that AE had anticancer capacity in high concentration alone, such as 10 µm concentration.[10] Aloe-emodin displayed stronger anticancer effect than 5-aminolevulinic acid as a new PS.[11]

Angiogenesis plays more and more important role in cancer metastasis.[12] Angiogenic factors attract endothelial cells and promote their proliferation. Proliferating and secreting proteases can induce endothelial cells to form blood vessels and enable them to migrate toward the tumor site by breaking open the blood vessels. Proliferating endothelial cells then form new capillary tubes. Finally, the capillaries provide a continuous blood flow for tumor cell metabolism and set up escaping avenues for metastatic tumor cells.[13-16]

The mitogen-activated protein kinase (MAPK) is a type of serine/threonine protein kinase.[17,18] Mitogen-activated protein kinase can be activated by growth factors, insulin, environmental factors, and cytokines, which involved in a broad intracellular response by the MAPK signaling pathway.[19,20] Studies found that MAPKs are involved in cell migration and invasion events partially.[21-23]

In this study, AE-PDT induced cell apoptosis and inhibited the proliferation, angiogenesis, migration, and invasion in human umbilical vein endothelial cells (HUVECs). At the same time, we found that AE-PDT can induce apoptosis of HUVECs by activating MAPK signaling pathway.

Materials and Method

Cells

Human umbilical vein endothelial cells (Shanghai Institute of Cell Biology, Shanghai, China) were cultured in Dulbecco's modified Eagle's medium (DMEM) medium (Hyclone, Logan, Utah) supplemented with 10% (vol/vol) fetal bovine serum (Bioind, Hyclone, Logan, Utah), 50 U/mL penicillin, and 50 μg/mL streptomycin (Hyclone, Logan, Utah) at 5% CO2 atmosphere and 37°C. Cells in the logarithmic growth phase were harvested for experiments.

Photosensitizer

Aloe-emodin (Jiangxi Herb Fine Co Ltd, Jiang Xi, China; purity >98%) was dissolved in dimethyl sulfoxide (Sigma-Aldrich, St Louis, Missouri) to prepare stock solution (100 mmol/L). The solutions were sterilized by filtration with a 0.22-μm membrane (Millipore, Bedford, Massachusetts) and stored at −20°C. The stock solution was diluted with DMEM medium (Hyclone) to the desired concentration.

Photodynamic Treatment

Four treatment groups were created: control, single AE, single light, and AE-PDT groups. Cells and AEs were cocultured for 6 hours and then washed 2 times by phosphate-buffered saline (PBS). Irradiations were performed using a blue LED device (430 nm) with a fluence rate of 40 mW/cm2; by adjusting the exposure time, the total light energy was 4, 8, 12, or 16 J/cm2: total light dose (J/cm2) = fluence rate (mW/cm2) × treatment time (seconds). Cells were maintained in complete medium during and posttreatment before assays.

Cell Viability

The viability of HUVECs was detected with the WST-8 assay (WST-8 Cell Proliferation Assay Kit, Dojindo, Japan). Absorbance was measured at 450 nm (Tecan Group Ltd, Mannedorf, Switzerland), and the percentage of survival cell was calculated.

5-Ethynyl-2-Deoxyuridine Assay

Cells were incubated with 50 µM 5-ethynyl-2-deoxyuridine (EdU labeling/detection kit; Ribobio, Guangzhou, China) for 12 hours and fixed with 4% paraformaldehyde (PA; pH 7.4) for 30 minutes. Cells were incubated with glycine for 5 minutes. Then anti-EdU solution was added into cells at room temperature and stored for 30 minutes after which cells were washed in PBS containing 0.5% Triton X-100. Finally, 5 μg/mL Hoechst 33342 was added to the cells and incubated for 30 minutes at room temperature to stain cellular nuclei and observed under a fluorescence microscope (Nikon, Japan).

Tube Formation Assay

Subconfluent HUVECs were harvested, resuspended in medium, and treated with AE-PDT. This suspension was seeded (70 000 cells/well) in Matrigel (Becton Dickinson, San Jose, California; Matrigel:free medium = 1:1) in a 96-well plate and incubated for 24 hours at 37°C with 5% CO2. Tube formation was examined under an inverted microscope and photographed at 40× magnification. Cumulative tube length was measured by using ImageJ software.[24] Results were shown as the mean and standard errors of triplicate experiments. [25]

Detection of ROS

Cells were cocultured with 2,7-dichlorodihydrofluorescein diactate (DCFH-DA) (Invitrogen, Paisley, United Kingdom) in a concentration of 10 μM for 30 minutes and washed twice with PBS. Then, the intracellular ROS were analyzed under fluorescence microscope (Nikon) and Fluor spectrophotometer (Tecan Group Ltd; Excitation = 488 nm, Emission = 520 nm).

Assay for Apoptosis

Cells treated with AE-PDT were resuspended with 500 µL binding buffer and then incubated with annexin fluorescein Isothiocyanate (V-FITC) and Propidium dioxide (PI) in a Ca2+-enriched binding buffer for 5 minutes. FACS Canto flow cytometer (Becton Dickinson) was used to determine whether the cellular death pathway was apoptotic or necrotic.

Mitochondrial Membrane Potential

5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolcarbocyanine iodide (JC-1) assay was performed to detect the mitochondrial membrane potential. Cells were incubated with 5 µg/mL of JC-1 (Invitrogen) for 30 minutes. Then, the membrane potential was measured in Spectra Fluor spectrophotometer (Tecan Group Ltd) and fluorescence microscopy (Nikon).

Western Blot Analysis

Cells were harvested and lysated with radio-Immunoprecipitation (RIPA) buffer (Sigma). Lysates were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and then transferred to nitrocellulose membranes (Millipore). Blots were incubated with primary antibodies and secondary antibodies. Antibodies used in this study included Bcl-2, caspase-9, matrix metalloproteinase (MMP) 2, and MMP9, from Santa Cruz Biotechnology (Dallas, Texas); P-38, extracellular signal-regulated kinase (ERK), the c-Jun N-terminal kinases (JNK), and vascular endothelial growth factor (VEGF) from Abcam (Cambridge, Massachusetts); and caspase-3 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from Cell Signaling Technologies. The blots were developed by an enhanced chemiluminescence (ECL) detection kit (Kaiji Bio Co, Nanjing, China) and visualized under LAS 3000 Luminoimage Analyzer. Three independent experiments were performed, and the representative results were shown.

Cell Migration and Invasion

The migration or invasive ability of cells was detected by Transwell chamber system (10-mm diameter of polycarbonate membrane with 8-mm pore size; Corning Costar, Cambridge, Massachusetts) coated with or without Matrigel (Becton Dickinson). Briefly, 50 µL Matrigel (Matrigel:free medium = 1:3) was added into transwell chambers and incubated at 37°C overnight. After fixation in 4% PA (Nanjing Sunshine Biotechnology Ltd, Nanjing, China), the cells invading through the membrane were stained with crystal violet (Nanjing Sunshine Biotechnology Ltd). The migratory and invasive cells were counted by microscope.

Subcellular Localization of AE

During the last 30 minutes of AE incubation, cells were coloaded with Mito-Tracker green at a final concentration of 500 nM to tag the mitochondria. An overlapped fluorescence from organelle probe and AE was observed under confocal microscopy (TCS SP5; Leica).

Cytoskeleton

Cells were seeded into a 96-well plate at a density of 1 × 105/mL for 24 hours and fixed with 4% PA (Nanjing Sunshine Biotechnology Ltd) for 10 minutes. Cells were permeabilized in PBS containing 0.5% Triton X-100 for 10 minutes, stained with Alexa Fluor 488 phalloidin (Life technologies) for 1 hour, and mounted by microscope.

Transmission Electron Microscopy

Cells were fixed in 0.1 M cacodylate buffer containing 2.5% glutaraldehyde for 2 hours at 4°C, followed by a second fixation in 0.1 M cacodylate buffer containing 1% osmium tetroxide for 1 hour at 4°C. After 3 washes in distilled H2O, the sample was stained with 0.5% aqueous uranyl acetate for 2 hours at room temperature. Transmission electron microscopy samples were observed using a JEOL1200EX instrument.

Statistical Analysis

Values were expressed as mean (standard deviation). The significance of differences between treatment groups was performed with analysis of variance test or 2-tailed Student t test. A value of P < .05 was considered statistically significant.

Results

Aloe-Emodin PDT-Suppressed Cell Proliferation

Treatment groups consisted of control, single AE, single light, and AE-PDT groups. Figure 1 shows changes in cell viability caused by different treatments. Aloe-emodin alone with a concentration of 20 μM and light irradiation alone with a density not less than 16 J/cm2 significantly decreased the cell survival (P < .05; Figure 1A). Thus, the concentration of AE was set at 15 μM, and the density of light irradiation was set at 12 J/cm2 in the following experiments of AE-PDT, which led to a cell survival rate of 56%. The population of EdU-positive cells was 35 ± 3, 34 ± 4, 32 ± 3, and 8 ± 2 in control, single AE, single light, and AE-PDT groups, respectively. The EdU-positive cells significantly declined in the AE-PDT group compared to the other 3 groups (P < .05; Figure 1B and C). No significant differences were observed among the control, single AE, and single light groups (P > .05).

Figure 1.

Aloe-emodin photodynamic therapy (AE-PDT) produced cytotoxicity and inhibited proliferation of human umbilical vein endothelial cells (HUVECs). A, The viability of cells was detected by the WST-8 assay. B, The expression of 5-ethynyl-2-deoxyuridine (EdU) was detected by immunofluorescence, and morphology was observed by phase-contrast visualization (EdU, ×400); EdU (red) and H-33342 (blue) staining of DNA. (a) Control group, (b) single AE group, (c) single light group, and (d) AE-PDT group. C, The number of EdU-positive cells. *The AE-PDT group versus control group, P < .05. #The AE-PDT group versus single AE group, P < .05. &The AE-PDT group versus single light group, P < .05. Values were represented as mean (standard deviation [SD]) of 3 independent determinations.

Aloe-Emodin PDT-Induced Apoptosis via the Mitochondria Pathway

Annexin V/PI staining demonstrated a significantly higher apoptotic percentage of death cells in the AE-PDT group (P < .05), with a value of 28.8%. No significant differences were observed among the other 3 groups (P > .05; Figure 2A and B). JC-1 staining demonstrated that a significant decrease in mitochondrial membrane potential was observed in the AE-PDT group (P < .05), whereas no significant differences were observed among the other 3 groups (P > .05; Figure 2C and D). Furthermore, the ratio of green to red fluorescence in the AE-PDT group increased to 2.3 times when compared to the control group. It is suggested that AE-PDT induces apoptotic cell death in HUVEC cells via the mitochondria pathway.

Figure 2.

A, Apoptosis detected by annexin V/PI double staining. B, The percentage of apoptosis. C, Mitochondrial membrane potential detected by the JC-1 assay (×200). D, The relative fluorescence intensity of mitochondrial membrane potential quantified by Fluor spectrophotometer. Scale bar was 50 µm. *Aloe-emodin photodynamic therapy (AE-PDT) group versus control group, P < .05. #The AE-PDT group versus single AE group, P < .05. &The AE-PDT group versus single light group, P < .05. Values were represented as mean (standard deviation [SD]) of 3 independent experiments.

Mito-tracker was used to view the subcellular localization of AE in the HUVECs. Overlapping regions (yellow) were regarded as the subcellular localization of AE. The results showed that the fluorescence images of the mitochondria probe were partially overlapping with the AE (Figure 3).

Figure 3.

Subcellular localization of aloe-emodin (AE) detected by confocal microscopy. Red fluorescent corresponds to AE, while green fluorescent shows Mito-Tracker with which shows the mitochondrion is stained. Yellow fluorescent indicates colocalization of red and green fluorescence.

The Expression of Caspase-9 and Caspase-3

The Western blot results showed that the expression of both caspase-9 and caspase-3 was significantly amplified after AE-PDT (P < .05; Figure 4). These data also suggest that AE-PDT induces apoptosis through the mitochondria pathway.

Figure 4.

A, Expression of caspase-9 and caspase-3. B-C, Quantitative analysis of protein expression according to panels. The vertical axis represents the gray value of caspase-9 and caspase-3. Each assay condition was done in triplicate. Data were represented as means (standard deviation [SD]).

Aloe-Emodin PDT Suppressed Cell Migration and Invasion

The average count of migratory cells in the AE-PDT group was 72 and significantly decreased, while the one in the control, single AE, and single light groups were 288, 286, and 282, respectively. The average number of invasive cells in the AE-PDT group was 19 and significantly declined when compared to the control, single AE, or single light groups, with values of 98, 93, and 92, respectively (P < .05; Figure 5A-C).

Figure 5.

Aloe-emodin photodynamic therapy (AE-PDT) inhibited the migration and invasion of human umbilical vein endothelial cells (HUVECs). A, The AE-PDT suppressed cell migration and invasion. The migration cells and invasion cells were stained by crystal violet (up; crystal violet, ×100). B, The AE-PDT inhibited cell migration. C, The AE-PDT inhibited cell invasion. (a) Control group, (b) single AE group, (c) single light group, (d) AE-PDT group. *The AE-PDT group versus control group, P < .05. #The AE-PDT group versus single AE group, P < .05. &The AE-PDT group versus single light group, P < .05. Each assay condition was done in triplicate. Data were represented as means (standard deviation [SD]).

Cytoskeleton Associated With Migration

Based on the data in cell migration, the effect of PDT on F-actin was explored using TRITC-phalloidin. F-actin in the AE-PDT group was shorter than that in the control, single AE, or single light groups, demonstrating a reduction in stress and the formation of aggregates (Figure 6).

Figure 6.

Cytoskeleton associated with migration F-actin(green) was detected by Phalloidin-TRITC. DNA(blue) was observed by Hochest-33342. Both were merged in HUVEC cells.

Aloe-Emodin PDT Negatively Regulates Angiogenesis

Matrigel tubule formation assay was used as an in vitro model to study the effect of AE-PDT on the capillary structure formation. As shown in Figure 7A, AE-PDT-treated cells reduced branching points, tubule number, and length. Simultaneously, the number of HUVEC capillary structures was significantly decreased by AE-PDT treatment (P < .05; Figure 7B). Taken together, these results indicate that AE-PDT may directly regulate angiogenesis in vitro.

Figure 7.

A, The effect of Aloe-emodin photodynamic therapy (AE-PDT) on the capillary structure formation was measured by using Matrigel tubule formation assay. (a) Control group, (b) Single AE group, (c) Single light group, (d) AE-PDT group. B, The numbers of formed capillary tubes in 4 groups were counted. *The AE-PDT group versus control group, P < .05. #The AE-PDT group versus single AE group, P < .05. &The AE-PDT group versus single light group, P < .05. Values were represented as means (standard deviation [SD]) of 3 independent determinations.

Mechanism of AE-PDT in Cell Deactivation Level of ROS

Reactive oxygen species mediates damages to mitochondria, thereby inducing apoptosis.[17] DCFH-DA staining showed that more ROS was significantly produced in the AE-PDT groups compared to the other 3 groups (P < .05; Figure 8B). The increase in ROS can be detected at 30 minutes and reached the peak at 1 hour after AE-PDT (Figure 8A and B). The data suggest that AE-PDT generates ROS resulting in apoptosis.

Figure 8.

A, Reactive oxygen species (ROS) observed by DCFH-DA staining (DCFH-DA, ×200). DCFH-DA is a nonfluorescent compound, but it can be hydrolyzed and then transferred to DCF, which is highly fluorescent when cells generate ROS. B, Levels of ROS at 30 minutes, 1 hour, and 1.5 hours after aloe-emodin photodynamic therapy (AE-PDT), detected by Fluor spectrophotometer. (a) Control group, (b) Single AE group, (c) Single light group, (d-f): AE-PDT group 30 minutes, 1 hour, and 1.5 hours. *The AE-PDT group versus control group, P < .05. #The AE-PDT group versus single AE group, P < .05. &The AE-PDT group versus single light group, P < .05. Values were represented as means (standard deviation [SD]) of 3 independent determinations.

The Expression of p38, p-p38, ERK, p-ERK, JNK, p-JNK, and VEGF

Compared to the other 3 groups, the expression of p-p38 and p-ERK ascended, while the expression of VEGF descended significantly in the AE-PDT group (P < .05). However, no significant difference was found in the expression of p-JNK among the 4 groups (P > .05; Figure 9). These data suggest that MAPK signaling pathways are possibly involved in the inhibition of metastasis of HUVECs induced by AE-PDT.

Figure 9.

The expression of p38, p-p38, ERK, p-ERK,JNK, p-JNK and VEGF. a: control, b:single AE group, c:single light group, d: AE-PDT group.

Discussion

Our data suggest that AE-PDT can inhibit cell proliferation, migration, invasion, and angiogenesis and induce apoptosis. We also delineated the mechanisms. On the one hand, ROS generated by AE-PDT triggers the HUVEC apoptosis through the mitochondria. On the other hand, MAPK signaling pathways triggered by ROS may be involved in the inhibition of angiogenesis, migration, and invasion in HUVEC induced by AE-PDT.

Photodynamic therapy has advantages such as minimal toxicity to normal tissues and lack of intrinsic or acquired resistance.[3] In general, the cardinal principium of PDT is that the PS is activated to generate ROS and their derivatives. Then, cellular oxidative stress, tissue anoxia, vasculature damage, and immune response are followed, which result in cell death.[26] An ideal PS, which plays a key role in PDT, should be ease of quality control, low costs as well as good stability, and have a high absorbance coefficient at 400 to 800 nm (blue to deep red). Aloe-emodin is such a novel compound, and the effects of AE-PDT require investigation.

Data in cytotoxic and EdU assays showed that AE-PDT suppressed cell proliferation. Annexin V/PI staining demonstrated a higher percentage of apoptotic cells in group AE-PDT. The apoptosis pathway was therefore explored. Given the short life and limited diffusion distance of AE in biological systems (half-life <0.04 microseconds and acting radius of action <0.02 μm), the primary site of photodamage coincides with the intracellular localization of AE.[27] Thus, we detected the subcellular localization of AE. Our data suggested that AE was localized mainly on the mitochondrion in HUVECs, which provoked us to seek the further events of mitochondrion. Interestingly, JC-1 staining showed a decrease in mitochondrial membrane potential in the AE-PDT group (Figure 2C-D). It is well known that, first the collapse of mitochondrial membrane potential recruits procaspase-9 to form a multimeric protein complex, subsequently the autoproteolytic activation of caspase-9 is followed, and then the activation of downstream of caspase-3 is initiated to complete the apoptosis process.[28-30] The collapse of mitochondrial membrane potential as well as increase in caspase-9 and caspase-3 found after AE-PDT in the present study demonstrates that AE-PDT induces apoptosis via the mitochondria pathways.

In the present study, the capacity of HUVEC migration and invasion was suppressed by AE-PDT treatment. Moreover, F-actin, a key factor involved in cell proliferation and migration, was also inhibited by the treatment of AE-PDT. It is now well established that migration and invasion take part in the metastasis[31]; simultaneously, the process of angiogenesis is essential to the growth of both primary and metastatic tumors.[32] Besides the inhibition of migration and invasion in HUVECs shown in the present study, the Matrigel tubule formation assay and the expression of VEGF demonstrated that the angiogenesis of HUVECs was also suppressed by AE-PDT. It has been found that many factors, including positive and negative ones, take part in the angiogenesis and metastasis.[33,34] The MAPK pathway has been demonstrated to serve a critical role in the angiogenesis and metastasis,[35,36] which provoked us to explore the change in MAPK pathway. The MAPK family consists of the 3 kinases: the ERK, the stress-activated protein kinase/JNK, and p38. The activity and the role of the MAPKs have, to some extent, been studied after PDT and are suspected to be of importance in the cellular processes controlling death and survival after oxidative stress.[37,38] Study showed that the p38 activation serves as a death signal, the JNK activation serves as a rescue signal, whereas the ERK activation has no influence on the treatment outcome.[39] Interestingly, our study showed that the expression of p-p38 and p-ERK ascended, while the expression of p-JNK did not change after AE-PDT. Otherwise, the link between MAPK pathway and cell angiogenesis as well as metastasis in colorectal cancer is well established.[23] It was indicated that activation of the Ras/Raf/MEK/ERK pathway was involved in the regulation of VEGF (which is important in angiogenesis) in human colorectal cancer.[21] Similarly, interactions between the cell surface urokinase plasminogen activator receptor and integrins are crucial for tumor invasion and metastasis, and urokinase plasminogen activator increases basal ERK activation.[22] The reduction in VEGF along with the augment of p-ERK and p-p38 was demonstrated in this study, indicating that both VEGF and ERK, p38 signaling pathways are involved in the suppression of angiogenesis and metastasis in HUVECs. Taken together, these results suggest that AE-PDT inhibits cell proliferation, migration, invasion, and angiogenesis in which the p38 and ERK MAPK signal pathways are involved.

Conclusion

In summary, AE-PDT induced cellular apoptotic cell death via the mitochondria pathways as well as inhibited cell migration, invasion, and angiogenesis via p38 and ERK MAPK signal pathways.