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Literature DB >> 3006672

Characteristics of complement subcomponents C1r and C1s synthesized by Hep G2 cells.

Abstract

The association and activation states of complement subcomponents C1r and C1s biosynthesized by Hep G2 cells were studied. C1r and C1s are secreted in stoichiometric amounts; in the presence of Ca2+ they are associated in a complex that sediments similarly to plasma C1r2-C1s2. Both compounds are synthesized as monomer proteins of apparent Mr 86 000. C1r is secreted as a dimer. Secreted C1r is not autoactivatable but undergoes proteolysis by exogenous C1r; secreted C1s is also proteolysed by exogenous C1r. In the presence of immune-complex-bound C1q, secreted C1r and C1s are able to reconstitute C1, but normal activation requires extrinsic C1r2-C1s2.

Entities: Chemical Gene

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Year: 1986 PMID： 3006672 PMCID： PMC1153062 DOI： 10.1042/bj2330559

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

15 in total

1. Fluid phase activation of proenzymic C1r purified by affinity chromatography.

Authors: C L Villiers; A M Duplaa; G J Arlaud; M G Colomb
Journal: Biochim Biophys Acta Date: 1982-01-04

2. The role of immune complexes in the activation of the first component of human complement.

Authors: R J Ziccardi
Journal: J Immunol Date: 1984-01 Impact factor: 5.422

3. A new role for C-1-inhibitor in homeostasis: control of activation of the first component of human complement.

Authors: R J Ziccardi
Journal: J Immunol Date: 1982-06 Impact factor: 5.422

4. Studies on the precursor form of the first component of complement. II. Proteolytic fragmentation of Clr.

Authors: S N Assimeh; R M Chapuis; H Isliker
Journal: Immunochemistry Date: 1978-01

5. Isolation and characterization of different forms of C1-r, a subcomponent of the first component of human complement.

Authors: K Okamura; S Fujii
Journal: Biochim Biophys Acta Date: 1978-06-21

6. Diamine-induced dissociation of the first component of human complement, C1.

Authors: C L Villiers; G J Arlaud; M G Colomb
Journal: Eur J Biochem Date: 1984-04-16

7. Purified proenzyme C1r. Some characteristics of its activation and subsequent proteolytic cleavage.

Authors: G J Arlaud; C L Villiers; S Chesne; M G Colomb
Journal: Biochim Biophys Acta Date: 1980-11-06

8. Complement biosynthesis by the human hepatoma-derived cell line HepG2.

Authors: K M Morris; D P Aden; B B Knowles; H R Colten
Journal: J Clin Invest Date: 1982-10 Impact factor: 14.808

9. Biosynthesis in vitro of complement subcomponents C1q, C1s and C1 inhibitor by resting and stimulated human monocytes.

Authors: J C Bensa; A Reboul; M G Colomb
Journal: Biochem J Date: 1983-11-15 Impact factor: 3.857

Review 10. The first component of human complement (C1): activation and control.

Authors: R J Ziccardi
Journal: Springer Semin Immunopathol Date: 1983

5 in total

1. Biosynthesis of complement C1 inhibitor by Hep G2 cells. Reactivity of different glycosylated forms of the inhibitor with C1s.

Authors: M H Prandini; A Reboul; M G Colomb
Journal: Biochem J Date: 1986-07-01 Impact factor: 3.857