Downregulation of vasopressin receptors in toad bladder.

Binding of tritium-labeled vasopressin [( 3H]AVP) to a broken epithelial cell preparation of the toad's urinary bladder has been related to hormonal action on water and urea transport across the intact tissue. Hormone binding to receptor sites and permeability changes were initiated at the same concentration of hormone (0.4 nM). Half-maximal urea and water permeability responses were observed with 3.1 and 5.6 nM AVP, respectively, although half-maximal receptor saturation required considerably higher concentrations of hormone (less than 500 nM). Because maximal permeability responses were obtained with occupation of approximately 200 fmol/mg protein receptor sites and the total receptor density was in excess of 2,000 fmol/mg protein, there is apparently a receptor reserve in this tissue. The antidiuretic hormone employed by the toad is vasotocin (AVT). This compound was 60-fold more effective than AVP in displacing [3H]AVP from receptor sites. Preincubation of bladders with AVT resulted in downregulation of receptor sites. Although the magnitude of receptor loss was equivalent in the presence or absence of a transmembrane osmotic pressure gradient, the capacity of AVT to induce permeability changes was more markedly reduced in the presence of an osmotic gradient. This observation suggests that the negative-feedback signal initiated by water flow through the hormone target cell diminishes sensitivity to hormone by a mechanism other than by a reduction in the number of surface receptors or by a decrease in their affinity for the hormone.