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Literature DB >> 3006444

Cycling S-phase cells in animal and spontaneous tumours. I. Comparison of the BrdUrd and 3H-thymidine techniques and flow cytometry for the estimation of S-phase frequency.

E M Langer, H R Röttgers, M G Schliermann, E M Meier, H G Miltenburger, J Schumann, W Göhde.

Abstract

Evaluation of the proliferative activities of cell populations has mainly been restricted to the use of autoradiography and flow cytometric measurements. The introduction of a new BrdUrd specific antibody makes it possible to determine exactly the DNA synthesizing cells. The BrdUrd technique is safe with respect to handling and the results are obtained within five hours. The suitability of the BrdUrd labelling procedure has been studied in different cell lines and compared with 3H-thymidine autoradiography and flow cytometry.

Entities: Chemical Disease

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Year: 1985 PMID： 3006444 DOI： 10.3109/02841868509134429

Source DB: PubMed Journal: Acta Radiol Oncol ISSN： 0349-652X

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Cited

8 in total

1. Microwave-aided technique to detect bromodeoxyuridine in S-phase cells using immunogold-silver staining and plastic-embedded sections.

Authors: H J van de Kant; M E Boon; D G De Rooij
Journal: Histochem J Date: 1988 Jun-Jul

2. Cellular proliferation in the anterior pituitary of the rat during the postnatal period.

Authors: E Carbajo-Pérez; Y G Watanabe
Journal: Cell Tissue Res Date: 1990-08 Impact factor: 5.249

3. A sequential double immunoenzymic staining procedure to obtain cell kinetic information in normal and hyperproliferative epidermis.

Authors: P E Van Erp; S De Mare; J J Rijzewijk; P C Van de Kerkhof; F W Bauer
Journal: Histochem J Date: 1989-06

4. Accurate determination of S-phase fraction in proliferative cells by dual fluorescence and peroxidase immunohistochemistry with 5-bromo-2'-deoxyuridine (BrdU) and Ki67 antibodies.

Authors: Rina Tanaka; Motomi Tainaka; Takumi Ota; Naoki Mizuguchi; Hiroyuki Kato; Shoichi Urabe; Yulin Chen; Jean-Michel Fustin; Yoshiaki Yamaguchi; Masao Doi; Shinshichi Hamada; Hitoshi Okamura
Journal: J Histochem Cytochem Date: 2011-05-06 Impact factor: 2.479

5. Effects of intralesional injection of cisplatin dissolved in urografin and lipiodol on Ehrlich ascites tumor and normal tissues of CD-1 mice.

Authors: J E Landrito; K Yoshiga; K Sakurai; K Takada
Journal: Cancer Chemother Pharmacol Date: 1994 Impact factor: 3.333

Cycling S-phase cells in animal and spontaneous tumours. I. Comparison of the BrdUrd and 3H-thymidine techniques and flow cytometry for the estimation of S-phase frequency.

1. Microwave-aided technique to detect bromodeoxyuridine in S-phase cells using immunogold-silver staining and plastic-embedded sections.

2. Cellular proliferation in the anterior pituitary of the rat during the postnatal period.

3. A sequential double immunoenzymic staining procedure to obtain cell kinetic information in normal and hyperproliferative epidermis.

4. Accurate determination of S-phase fraction in proliferative cells by dual fluorescence and peroxidase immunohistochemistry with 5-bromo-2'-deoxyuridine (BrdU) and Ki67 antibodies.

5. Effects of intralesional injection of cisplatin dissolved in urografin and lipiodol on Ehrlich ascites tumor and normal tissues of CD-1 mice.

6. The applications of the BrdUrd-technique for the estimation of cycling S-phase cells in human renal cell carcinoma.

7. DNA cell cycle studies in uveal melanoma.

8. Simple Laboratory methods to measure cell proliferation using DNA synthesis property.