Cinzia Bersani1, Linnea Haeggblom1, Ramona G Ursu1,2, Simona E Giusca2, Linda Marklund3, Torbjörn Ramqvist1, Anders Näsman1,4, Tina Dalianis5. 1. Department of Oncology-Pathology, Karolinska Institutet, Stockholm, Sweden. 2. University of Medicine and Pharmacy Grigore T. Popa, Iasi, Romania. 3. CLINTEC, Karolinska Institutet, Stockholm, Sweden. 4. Department of Clinical Pathology, Karolinska University Hospital, Stockholm, Sweden. 5. Department of Oncology-Pathology, Karolinska Institutet, Stockholm, Sweden Tina.Dalianis@ki.se.
Abstract
BACKGROUND/AIM: Human papillomavirus-positive (HPV+) tonsillar and base of tongue squamous cell carcinoma (TSCC/BOTSCC) have better outcome than corresponding HPV- cancers. To better individualize treatment, additional predictive markers are needed. Previously, we have shown that mutated fibroblast growth factor receptor 3 protein (FGFR3) was correlated to poorer prognosis and here FGFR3 expression was further analyzed. PATIENTS AND METHODS: One-hundred-fifteen HPV+TSCC/ BOTSCC biopsies were analyzed for FGFR3 by immunohistochemistry (IHC), and 109/115 were analyzed for FGFR3 mutations by Ion Proton sequencing, or by Competitive Allele-Specific Taqman PCR (CAST-PCR). Disease-free survival (DFS) was then calculated according to FGFR3 IHC expression. RESULTS: CAST-PCR was useful for detecting the three most common FGFR3 mutations. Focusing especially on the 98/115 patients with HPV+TSCC/BOTSCC and wild-type FGFR3, high FGFR3 expression correlated to significantly better 3-year DFS, p=0.043. CONCLUSION: In patients with HPV+TSCC/BOTSCC and wild-type FGFR3, overexpression of FGFR3 was correlated with better DFS. Copyright
BACKGROUND/AIM: Human papillomavirus-positive (HPV+) tonsillar and base of tongue squamous cell carcinoma (TSCC/BOTSCC) have better outcome than corresponding HPV- cancers. To better individualize treatment, additional predictive markers are needed. Previously, we have shown that mutated fibroblast growth factor receptor 3 protein (FGFR3) was correlated to poorer prognosis and here FGFR3 expression was further analyzed. PATIENTS AND METHODS: One-hundred-fifteen HPV+TSCC/ BOTSCC biopsies were analyzed for FGFR3 by immunohistochemistry (IHC), and 109/115 were analyzed for FGFR3 mutations by Ion Proton sequencing, or by Competitive Allele-Specific Taqman PCR (CAST-PCR). Disease-free survival (DFS) was then calculated according to FGFR3 IHC expression. RESULTS: CAST-PCR was useful for detecting the three most common FGFR3 mutations. Focusing especially on the 98/115 patients with HPV+TSCC/BOTSCC and wild-type FGFR3, high FGFR3 expression correlated to significantly better 3-year DFS, p=0.043. CONCLUSION: In patients with HPV+TSCC/BOTSCC and wild-type FGFR3, overexpression of FGFR3 was correlated with better DFS. Copyright
Authors: Stefan Holzhauser; Ourania N Kostopoulou; Anna Ohmayer; Birthe K A Lange; Torbjörn Ramqvist; Teodora Andonova; Cinzia Bersani; Malin Wickström; Tina Dalianis Journal: Oncol Lett Date: 2019-10-09 Impact factor: 2.967
Authors: Andreas Ährlund-Richter; Stefan Holzhauser; Tina Dalianis; Anders Näsman; Michael Mints Journal: Cancers (Basel) Date: 2021-12-24 Impact factor: 6.639
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