Huifeng Xu1, Tao Qing2, Yi Shen3, Junling Huang3, Yang Liu3, Jian Li3, Timing Zhen4, Kaichen Xing4, Sibo Zhu2, Ming Luo5. 1. Department of Cardiology, Tongji Hospital of Tongji University, Shanghai, China. 2. School of Life Sciences, Fudan University, Shanghai 200438, China; Shanghai Cinoasia Institute, Shanghai 200438, China. 3. Department of Geriatrics, Tongji Hospital of Tongji University, Shanghai, China. 4. Shanghai Cinoasia Institute, Shanghai 200438, China. 5. Department of Cardiology, Tongji Hospital of Tongji University, Shanghai, China. Electronic address: luoming_tj@163.com.
Abstract
OBJECTIVES: High-salt diet is one of the major risk factors in the development of hypertension. Previous studies have observed a relationship between high-salt induced blood pressure levels and cardiac-cerebral disease. However, the molecular mechanism of high-salt diet induced hypertension and the serious complications in cardiovascular system still remain unknown. MATERIALS AND METHODS: We built high-salt diet induced hypertension rat models, and investigated the transcriptomic alteration in four hypertension affected tissues, i.e. ventricle and atrium in heart as well as cortex and medulla in kidney. Differential expression gene (DEG) analysis and further functional annotation including Ingenuity Pathway Analysis (IPA) was performed to reveal the molecular mechanism of high-salt induced hypertension and organ injury. RESULTS: We observed that several genes associated with cardiovascular development and organ injury were significantly dysregulated rat fed with high-salt diet, such as Mmp-15, Igfbp7, Rgs18 and Hras. We demonstrated that differential expressed genes were functionally related to the increased levels of alkaline phosphatase (ALP). CONCLUSION: Our study provided new insight about molecular mechanism of high-salt induced hypertension and heart and kidney damage.
OBJECTIVES: High-salt diet is one of the major risk factors in the development of hypertension. Previous studies have observed a relationship between high-salt induced blood pressure levels and cardiac-cerebral disease. However, the molecular mechanism of high-salt diet induced hypertension and the serious complications in cardiovascular system still remain unknown. MATERIALS AND METHODS: We built high-salt diet induced hypertensionrat models, and investigated the transcriptomic alteration in four hypertension affected tissues, i.e. ventricle and atrium in heart as well as cortex and medulla in kidney. Differential expression gene (DEG) analysis and further functional annotation including Ingenuity Pathway Analysis (IPA) was performed to reveal the molecular mechanism of high-salt induced hypertension and organ injury. RESULTS: We observed that several genes associated with cardiovascular development and organ injury were significantly dysregulated rat fed with high-salt diet, such as Mmp-15, Igfbp7, Rgs18 and Hras. We demonstrated that differential expressed genes were functionally related to the increased levels of alkaline phosphatase (ALP). CONCLUSION: Our study provided new insight about molecular mechanism of high-salt induced hypertension and heart and kidney damage.
Authors: Justine M Abais-Battad; Ammar J Alsheikh; Xiaoqing Pan; Daniel J Fehrenbach; John Henry Dasinger; Hayley Lund; Michelle L Roberts; Alison J Kriegel; Allen W Cowley; Srividya Kidambi; Theodore A Kotchen; Pengyuan Liu; Mingyu Liang; David L Mattson Journal: Hypertension Date: 2019-09-03 Impact factor: 10.190
Authors: Merle M Krebber; Christian G M van Dijk; Robin W M Vernooij; Maarten M Brandt; Craig A Emter; Christoph D Rau; Joost O Fledderus; Dirk J Duncker; Marianne C Verhaar; Caroline Cheng; Jaap A Joles Journal: Int J Mol Sci Date: 2020-09-14 Impact factor: 5.923