| Literature DB >> 30059039 |
Yanlan Wang1, Jiayu Liu1, Haitao Pan1, Jieyu Xing1, Xiaoqiong Wu1, Qing Li2, Zhong Wang3.
Abstract
This protocol describes the construction and functional studies of a bispecific antibody (bsAb), GPC3-S-Fab. bsAbs can recognize two different epitopes through their two different arms. bsAbs have been actively studied for their ability to directly recruit immune cells to kill tumor cells. Currently, the majority of bsAbs are produced in the form of recombinant proteins, either as Fc-containing bsAbs or as smaller bsAb derivatives without the Fc region. In this study, GPC3-S-Fab, an antibody fragment (Fab) based bispecific antibody, was designed by linking the Fab of anti-GPC3 antibody GC33 with an anti-CD16 single domain antibody. The GPC3-S-Fab can be expressed in Escherichia coli and purified by two affinity chromatographies. The purified GPC3-S-Fab can specifically bind to and kill GPC3 positive liver cancer cells by recruiting natural killer cells, suggesting a potential application of GPC3-S-Fab in liver cancer therapy.Entities:
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Year: 2018 PMID： 30059039 PMCID： PMC6126450 DOI： 10.3791/57588
Source DB: PubMed Journal: J Vis Exp ISSN： 1940-087X Impact factor: 1.355