Literature DB >> 30059028

Subcellular Fractionation from Fresh and Frozen Gastrointestinal Specimens.

Irati Romero-Garmendia1, Amaia Jauregi-Miguel1, Izortze Santin2, Jose Ramón Bilbao1, Ainara Castellanos-Rubio3.   

Abstract

The purpose of this protocol is to fractionate human intestinal tissue obtained by endoscopy into nuclear and cytoplasmic compartments for the localization analysis of specific proteins or protein complexes in different tissue states (i.e., healthy vs. disease). This method is useful for the fractionation of both fresh and frozen intestinal tissue samples; it is easily accessible for all laboratories and not time consuming.

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Year:  2018        PMID： 30059028      PMCID： PMC6126469          DOI： 10.3791/57740

Source DB:  PubMed          Journal:  J Vis Exp        ISSN： 1940-087X            Impact factor:   1.355


  11 in total

1.  A mammalian organelle map by protein correlation profiling.

Authors:  Leonard J Foster; Carmen L de Hoog; Yanling Zhang; Yong Zhang; Xiaohui Xie; Vamsi K Mootha; Matthias Mann
Journal:  Cell       Date:  2006-04-07       Impact factor: 41.582

Review 2.  Frozen tissue biobanks. Tissue handling, cryopreservation, extraction, and use for proteomic analysis.

Authors:  Christer Ericsson; Bo Franzén; Monica Nistér
Journal:  Acta Oncol       Date:  2006       Impact factor: 4.089

3.  Tissue subcellular fractionation and protein extraction for use in mass-spectrometry-based proteomics.

Authors:  Brian Cox; Andrew Emili
Journal:  Nat Protoc       Date:  2006       Impact factor: 13.491

4.  Subcellular protein extraction from human pancreatic cancer tissues.

Authors:  Anette Börner; Uwe Warnken; Martina Schnölzer; Jörg von Hagen; Nathalia Giese; Andrea Bauer; Jörg Hoheisel
Journal:  Biotechniques       Date:  2009-04       Impact factor: 1.993

5.  Global, quantitative and dynamic mapping of protein subcellular localization.

Authors:  Daniel N Itzhak; Stefka Tyanova; Jürgen Cox; Georg Hh Borner
Journal:  Elife       Date:  2016-06-09       Impact factor: 8.140

6.  New application of a subcellular fractionation method to kidney and testis for the determination of conjugated linoleic acid in selected cell organelles of healthy and cancerous human tissues.

Authors:  Kristina Hoffmann; Jörg Blaudszun; Claus Brunken; Wilhelm-Wolfgang Höpker; Roland Tauber; Hans Steinhart
Journal:  Anal Bioanal Chem       Date:  2005-03-11       Impact factor: 4.142

7.  Differential localization of T-bet and Eomes in CD8 T cell memory populations.

Authors:  Laura M McLane; Pinaki P Banerjee; Gabriela L Cosma; George Makedonas; E John Wherry; Jordan S Orange; Michael R Betts
Journal:  J Immunol       Date:  2013-03-01       Impact factor: 5.422

Review 8.  Applications of proteomics in the study of inflammatory bowel diseases: Current status and future directions with available technologies.

Authors:  Philip Alex; Marjan Gucek; Xuhang Li
Journal:  Inflamm Bowel Dis       Date:  2009-04       Impact factor: 5.325

Review 9.  Mechanisms of disease: protease functions in intestinal mucosal pathobiology.

Authors:  Toni M Antalis; Terez Shea-Donohue; Stefanie N Vogel; Cynthia Sears; Alessio Fasano
Journal:  Nat Clin Pract Gastroenterol Hepatol       Date:  2007-07

10.  Sequential fractionation and isolation of subcellular proteins from tissue or cultured cells.

Authors:  Sabina Baghirova; Bryan G Hughes; Michael J Hendzel; Richard Schulz
Journal:  MethodsX       Date:  2015-11-07
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