Literature DB >> 30058482

Changes in the Expression Profile of JAK/STAT Signaling Pathway Genes and Mirnas Regulating their Expression Under the Adalimumab Therapy.

Beniamin Grabarek1, Dominika Wcislo-Dziadecka2, Joanna Gola1, Celina Kruszniewska-Rajs1, Ligia Brzezinska-Wcislo3, Nikola Zmarzly1, Urszula Mazurek1.   

Abstract

OBJECTIVE: The aim of this study was to evaluate the influence of adalimumab on the expression pattern of genes associated with JAK/STAT signaling pathway in Normal Human Dermal Fibroblast (NHDF) cells stimulated with 8 µg/ml of adalimumab and the identification of miRNAs regulating these genes' expression.
METHOD: NHDFs were cultured with or without the presence of adalimumab for 2, 8, and 24h. The microarray technology was used to determine expression profile of mRNAs and miRNAs.
RESULTS: Out of 22283 ID mRNA, 37 are associated with the JAK/STAT signaling pathway. It can be observed that 18 mRNAs differentiate NHDFs cultures with adalimumab from control. The analysis of miRNAs showed that, among 1105 ID miRNAs, 20 miRNAs are differentiating in cells treated with adalimumab for 2h, 9 miRNAs after 8h, and only 3 miRNAs after 24h.
CONCLUSION: It can be observed that miRNAs play an extremely important role in the regulation of the expression of genes associated with JAK/STAT signaling pathway. The results of this study show the possibility of using changes in mRNAs and miRNAs profile expression, as complementary molecular markers of adalimumab treatment effectiveness. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

Entities:  

Keywords:  JAK/STAT signaling pathway; NHDF cultures; adalimumab; interleukin; loss of sensitivity to treatment; micro RNA.

Mesh：

Substances：

Year:  2018        PMID： 30058482     DOI： 10.2174/1389201019666180730094046

Source DB:  PubMed          Journal:  Curr Pharm Biotechnol        ISSN： 1389-2010            Impact factor:   2.837


  6 in total

1.  In silico analysis of CpG islands and miRNAs potentially regulating the JAK-STAT signalling pathway.

Authors:  Beniamin O Grabarek; Dominika Wcisło-Dziadecka; Joanna Gola
Journal:  Postepy Dermatol Alergol       Date:  2020-09-02       Impact factor: 1.837

2.  Computational analysis for identification of the extracellular matrix molecules involved in endometrial cancer progression.

Authors:  Vijesh Kumar Yadav; Tzong-Yi Lee; Justin Bo-Kai Hsu; Hsien-Da Huang; Wei-Chung Vivian Yang; Tzu-Hao Chang
Journal:  PLoS One       Date:  2020-04-21       Impact factor: 3.240

3.  MicroRNA global profiling in cystic fibrosis cell lines reveals dysregulated pathways related with inflammation, cancer, growth, glucose and lipid metabolism, and fertility: an exploratory study.

Authors:  Cecilia Catellani; Francesca Cirillo; Sara Graziano; Luisa Montanini; Nelson Marmiroli; Mariolina Gullì; Maria Elisabeth Street
Journal:  Acta Biomed       Date:  2022-07-01

4.  The Influence of Adalimumab and Cyclosporine A on the Expression Profile of the Genes Related to TGFβ Signaling Pathways in Keratinocyte Cells Treated with Lipopolysaccharide A.

Authors:  Iwona Adwent; Beniamin Oskar Grabarek; Marta Kojs-Mrożkiewicz; Ryszard Brus; Rafał Staszkiewicz; Andrzej Plewka; Michał Stasiowski; Anita Lyssek-Boroń
Journal:  Mediators Inflamm       Date:  2020-07-26       Impact factor: 4.711

5.  Evaluation of the Impact of Cisplatin on Variances in the Expression Pattern of Leptin-Related Genes in Endometrial Cancer Cells.

Authors:  Dariusz Dąbruś; Robert Kiełbasiński; Beniamin Oskar Grabarek; Dariusz Boroń
Journal:  Int J Mol Sci       Date:  2020-06-10       Impact factor: 5.923

6.  Evaluation of the Influence of Adalimumab on the Expression Profile of Leptin-Related Genes and Proteins in Keratinocytes Treated with Lipopolysaccharide A.

Authors:  Beniamin Oskar Grabarek; Tomasz Kasela; Iwona Adwent; Barbara Zawidlak-Węgrzyńska; Ryszard Brus
Journal:  Int J Mol Sci       Date:  2021-02-05       Impact factor: 5.923
  6 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.