| Literature DB >> 3005434 |
J E Groopman, F W Chen, J A Hope, J M Andrews, R L Swift, C V Benton, J L Sullivan, P A Volberding, D P Sites, S Landesman.
Abstract
Current efforts to test blood donors and other persons for exposure to the human T cell lymphotropic virus type III (HTLV-III), the etiologic agent of the acquired immunodeficiency syndrome (AIDS), are based on the measurement of serum antibodies to viral antigens. We studied presence of serum antibodies to HTLV-III-related antigens from 767 individuals with AIDS or AIDS-related complex (ARC) or asymptomatic persons at risk for AIDS by using ELISA and immunoblot techniques. Of the 280 specimens from AIDS and ARC subjects that were tested, 99% were ELISA reactive and 96% were immunoblot reactive. Greater than 96% of the seropositive subjects manifested antibodies to the p24 core antigen, whereas only 88% had antibodies to the gp41 envelope-related glycoprotein. Contrary to previous reports, a short incubation time in the immunoblot assay failed to detect low-titer or low-affinity antibodies that were detected by overnight incubation. There was no apparent difference in pattern of antibodies to HTLV-III-related antigens in symptomatic vs. asymptomatic seropositive individuals.Entities:
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Year: 1986 PMID： 3005434 DOI： 10.1093/infdis/153.4.736
Source DB: PubMed Journal: J Infect Dis ISSN： 0022-1899 Impact factor: 5.226