Generation of Ultra-Small PLGA Nanoparticles by Sequential Centrifugation.

Direct, local delivery of polymer nanoparticles to the brain is a promising strategy to bypass the blood-brain barrier (BBB) and safely deliver a large therapeutic payload. However, even with the aid of convection-enhanced delivery (CED) techniques, this approach has been limited by the inability to fabricate appropriately sized polymer nanoparticles. Here, we outline a versatile and efficient method for producing polymer nanoparticles that are <100 nm in diameter and can be delivered to the brain via CED.