Literature DB >> 3005131

A common precursor for the two subunits of the penicillin acylase from Escherichia coli ATCC11105.

G Oliver, F Valle, F Rosetti, M Gómez-Pedrozo, P Santamaría, G Gosset, F Bolivar.   

Abstract

Penicillin acylase (PA) is an industrial enzyme that is used to convert penicillin G into a precursor for semisynthetic penicillins. We have cloned a segment of DNA that codes for the two subunits required for PA activity. We also report the nucleotide sequence of a DNA fragment that codes for (i) the small subunit, (ii) the N-terminal region of the large subunit and (iii) a putative connecting peptide. These results confirm the existence of a common precursor for both peptides.

Entities:  

Mesh：

Substances：

Year:  1985        PMID： 3005131     DOI： 10.1016/0378-1119(85)90018-6

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  4 in total

1.  Penicillin acylase from E. coli: unique gene-protein relation.

Authors:  G Schumacher; D Sizmann; H Haug; P Buckel; A Böck
Journal:  Nucleic Acids Res       Date:  1986-07-25       Impact factor: 16.971

2.  Periplasmic aggregation limits the proteolytic maturation of the Escherichia coli penicillin G amidase precursor polypeptide.

Authors:  S Scherrer; N Robas; H Zouheiry; G Branlant; C Branlant
Journal:  Appl Microbiol Biotechnol       Date:  1994-10       Impact factor: 4.813

3.  Penicillin acylase mutants with altered site-directed activity from Kluyvera citrophila.

Authors:  I Prieto; J Martín; R Arche; P Fernández; A Pérez-Aranda; J L Barbero
Journal:  Appl Microbiol Biotechnol       Date:  1990-08       Impact factor: 4.813

4.  IS2-mediated re-arrangement of the promoter sequence suppresses metabolic burden of the recombinant plasmid.

Authors:  R Valesová; V Stepánek; B Vecerek; P Kyslík
Journal:  Folia Microbiol (Praha)       Date:  2005       Impact factor: 2.629
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.