Clinical and quantitative computed tomography predictors of response to endobronchial lung volume reduction therapy using coils.

Objectives: Bronchoscopic lung volume reduction using coils (LVRC) is a well-known treatment option for severe emphysema. The purpose of this study was to identify quantitative computed tomography (QCT) and clinical parameters associated with positive treatment outcome. Patients and methods: The CT scans, pulmonary function tests (PFT), and 6-minute walk test (6-MWT) data were collected from 72 patients with advanced emphysema prior to and at 3 months after LVRC treatment. The procedure involved placing 10 coils unilaterally. Various QCT parameters were derived using Apollo imaging software (VIDA). Independent predictors of clinically relevant outcome (Δ6-MWT ≥ 26 m, ΔFEV1 ≥ 12%, ΔRV ≥ 10%) were identified through stepwise linear regression analysis.
Results: The response outcome for Δ6-MWT, for ΔFEV1 and for ΔRV was met by 55%, 32% and 42%, respectively. For Δ6-MWT ≥ 26 m a lower baseline 6-MWT (p = 0.0003) and a larger standard deviation (SD) of low attenuation cluster (LAC) sizes in peripheral regions of treated lung (p = 0.0037) were significantly associated with positive outcome. For ΔFEV1 ≥ 12%, lower baseline FEV1 (p = 0.02) and larger median LAC sizes in the central regions of treated lobe (p = 0.0018) were significant predictors of good response. For ΔRV ≥ 10% a greater baseline TLC (p = 0.0014) and a larger SD of LAC sizes in peripheral regions of treated lung (p = 0.007) tended to respond better.
Conclusion: Patients with lower FEV1 and 6-MWT, with higher TLC and specific QCT characteristics responded more positively to LVRC treatment, suggesting a more targeted CT-based approach to patient selection could lead to greater efficacy in treatment response.

Plain language summary

Our aim was to investigate and recognize possible quantitative computed tomography and/or clinical parameters which might be associated with positive lung volume reduction outcomes after endobronchial coils implantation (LVRC) in patients with severe heterogeneous emphysema. Our results showed that such predictors do exist. Taking those predictors into account, a more targeted approach to patient selection could lead to greater efficacy in treatment response.

Introduction

Emphysema is often a key component of COPD and is characterized by irreversible lung tissue destruction and loss of elasticity. It leads to air trapping and hyperinflation causing progressively worsening dyspnea and exercise capacity, significantly impairing the patient’s quality of life.1

In the past decade, different approaches for endoscopic lung volume reduction (ELVR) have been developed. These approaches differ in indication, mechanism of action, reversibility, and complications, and are divided into blocking and nonblocking techniques. ELVR by means of endobronchial valve placement has the most widespread clinical use to date.2–6 However, valve placement is only successful in patients with absent interlobar collateral ventilation. Bronchoscopic lung volume reduction using coils (PneumRx Inc., Mountain View, CA, USA), is a nonblocking approach for ELVR and thus independent of collateral ventilation. Since the first publication of coil implantation by Herth et al in 2010,7 several trials have reported encouraging results regarding its effectiveness.8–11

Patient selection for ELVR has been modeled on the National Emphysema Treatment Trial (NETT)11,12 while the optimal criteria currently used are summarized in an expert recommendation panel from the year 2017 (FEV1 < 50%, RV < 175%, RV/TLC < 0.58, 6-MWT 150–450 m).13 However, in order to identify optimal candidates for each ELVR approach and thus improve treatment outcome, dedicated screening is necessary. Until recently, little was known about the specific characteristics of subjects more likely to respond better to endoscopic lung volume reduction treatment using coils (LVRC). In the biggest randomized controlled trial (RCT) related to coil therapy, the RENEW trial,14 the residual volume and the emphysema distribution were found to be predictors for successful coil treatment. Although routine visual analysis of emphysema can be performed with a good degree of confidence using computed tomography (CT) images, experienced readers might be required for a more reliable assessment. To increase the consistency of the assessment, automated software solutions are available for quantifying emphysema destruction and distribution using quantitative CT (QCT).

Various QCT predictors of ELVR treatment outcome using valves have been evaluated so far. Low-attenuation area percentage (LAA%), heterogeneity score and fissure integrity (FI) score15–17 appear to aid in the selection process of patients and lobe for valve placement. However, it is still unknown if QCT measures are also predictors of LVRC therapy. In our retrospective study, we sought to determine QCT and clinical predictors of LVRC outcome to objectively guide and improve the selection of subjects.

Patients and methods

Patients and study design

Seventy-two patients (male/female: 35/37, mean age: 63 ± 6.7 years) who underwent unilateral LVRC treatment at the Thoraxklinik at university of Heidelberg were analyzed retrospectively. This study was a single-center analysis of a patient cohort which was treated with LVRC outside of clinical trials. Patients with severe heterogeneous emphysema and bilateral incomplete interlobar fissures were included. The study protocol was approved by the ethics committee of the University of Heidelberg (ethics number S-609/2012). All patients gave consent for the scientific use of the data acquired during hospitalization. Furthermore, the majority of the patients were treated within different prospective trials for endoscopic lung volume reduction after additional written consent. As the data in this current analysis were retrospectively analyzed no further patient consent was required. All subjects were ex-smokers who quit smoking at least 4 months before coil placement. The patients were deemed suitable candidates for LVRC according to previously described inclusion and exclusion criteria for ELVR.2

The pulmonary function tests (PFTs) were performed using the Erich Jaeger MasterLab Plethysmograph, per current American Thoracic Society/European Respiratory Society guidelines, and for the static volumes the reference values ECCS93 were used. PFT, 6-minute walk test (6-MWT), dyspnea score, image data from multi detector CT (MDCT) scan, a perfusion scan and echocardiogram were performed prior to and 3 months following the coil therapy.18,19

A combination of rigid and flexible bronchoscopy under general anesthesia was performed as per hospital standard. A total of 10 coils were placed in a single lobe and in a single session under fluoroscopic guidance into the sub-segmental bronchi of the lobe most affected by emphysema per MDCT evaluation (Figure 1). Details of the coil design, sizes, function, and insertion technique have been described previously.7 Patients received intravenous antibiotic prophylaxis and remained in hospital under observation for 3–4 days on average.

Figure 1

X-ray of a patient with coils in the right upper lobe.

Responder analysis

A positive treatment outcome for lung volume reduction (LVR), was defined as LVR ≥ 350 mL (lobar volume difference greater than 350 mL between baseline and follow-up) as previously described.2,15 An increase in FEV1 by greater than 12% (ΔFEV1 ≥ 12%) and a decrease in RV by greater than 10% (ΔRV ≥ 10%) were found in previous studies to indicate the minimal clinically important difference (MCID).8,20 Finally, an increase of 26 m or greater in the 6-MWT (Δ6-MWT ≥ 26 m) was found to signify MCID from previous studies.21,22

MDCT image acquisition and analysis

All MDCT scans were acquired at full inspiration and expiration at baseline and 90 days after treatment. Acquisition of the non-enhanced CT images was achieved using Siemens Somatom Definition AS 64 or Siemens Emotion 6 scanners (Siemens, Erlangen, Germany). Acquisition parameters for the scanners were set to: kVp = [100-120], effective mAs = [51-151], pitch = 1.5, reconstruction kernel = I40 f, dose modulation = ON, slice spacing = 0.7 mm, and slice thickness = 1 mm during an inspiratory and expiratory breath-hold, respectively.

CT images were evaluated using the Apollo 2.0 software package and the VIDA Diagnostics, Inc. clinical image analysis service that is ISO13485 certified for quality control (Coralville, Iowa, USA). Low attenuation areas (LAAs) of the lung below the threshold of −950 Hounsfield units (HU) were used to quantify the severity of emphysema. Emphysema percentage (EP) is the fraction of LAA found in each lobe. Heterogeneity score (HS) is the difference between EP of the treated lobe and its ipsilateral lobe. A value of > 15% points is deemed to be heterogeneous disease whereas a value of ≤ 15% points is homogeneous. Low attenuation cluster (LAC) that reflect the size of the “emphysematous holes” is calculated by plotting the number of LAA clusters against the size of the clusters and taking the slope.23 LAC by lobes and central vs peripheral were reported.15 The LAC slope as well as the median size and the SD of LAC sizes was evaluated. A steep slope indicates fewer “big clusters” or more small emphysematous holes, possibly indicating early disease or diffuse pan-lobular emphysema while the SD of LAC is a possible indicator of disease homogeneity with a higher SD indicating more distributed disease. Air trapping was measured as the percentage of low attenuation areas that fell below −856 (HU) on the expiratory CT image. Finally, peripheral pulmonary vessel volume was measured as the percentage of volume of the small vessels of the segmented patient vessel tree relative to the overall vessel tree volume.

Statistical analysis

Stepwise linear regression analysis was used to classify independent predictors of positive clinical outcome to LVRC treatment. QCT baseline measurements were first put through a correlation analysis with outcome measures to simplify the QCT model. This simplified QCT model, baseline PFT and baseline 6-MWT, were run through a multiple regression analysis to find the various predictors of clinical relevant outcome (response criteria Δ6-MWT ≥ 26 m, ΔFEV1 ≥ 12%, and ΔRV ≥ 10%).

Results

Baseline characteristics of the patients are shown in Table 1. Some 55% of our procedures involved the right upper lobe, 15% the left upper lobe, another 15% the left lower lobe and finally 13% involved the right lower lobe. No coils were placed in the right middle lobe and all patients were treated unilaterally. No periprocedural technical problems occurred.

Table 1

Baseline characteristics of all patients together as well as of the responder and non-responder groups separately (A), accompanied by the response rates at 90-day follow-up (B)

A
Characteristics	Baseline
	All	Responder			Non-responder			Significance of difference (p)
		FEV1	RV	6-MWT	FEV1	RV	6-MWT	FEV1	RV	6-MWT
Male/female	35/37
Age, years	63 (7)	63.65 (6.6)	65 (6)	64 (7)	63.55 (6.96)	62 (7)	62 (7)	0.95	0.11	0.28
Lung function
FEV1, Lt	0.70 (0.21)	0.67 (0.19)	0.73 (0.22)	0.71 (0.19)	0.72 (0.21)	0.68 (0.19)	0.70 (0.22)	0.37	0.35	0.94
FEV1, % pred	27 (8)	27 (8)	27 (6)	27 (8)	28 (7)	27 (8)	28 (6)	0.63	0.97	0.73
FVC, Lt	2.06 (0.69)	2.07 (0.73)	2.13 (0.83)	2.0 (0.67)	2.06 (0.70)	2.03 (0.59)	2.1 (0.78)	0.97	0.54	0.69
FVC, % pred	61 (17)	63 (20)	63 (16)	60 (18)	61 (16)	61 (19)	64 (14)	0.54	0.73	0.34
RV, Lt	5.98 (1.49)	6.10 (1.59)	6.50 (1.47)	6.06 (1.44)	6.04 (1.24)	5.75 (1.21)	5.93 (1.26)	0.87	0.02	0.69
RV, % pred	277 (57)	280 (53)	288 (49)	275 (45)	280 (47)	275 (59)	284 (55)	0.97	0.29	0.51
TLC, Lt	8.10 (1.79)	8.18 (1.64)	8.66 (1.73)	8.11 (1.54)	8.22 (1.70)	7.79 (1.43)	8.07 (1.64)	0.91	0.03	0.91
RV/TLC, ratio	73 (10)	74 (9)	75 (8)	74 (8)	75 (6)	74 (7)	74 (7)	0.75	0.41	0.62
Exercise capacity
6-MWT, m	239 (93)	224 (105)	235 (113)	216 (92)	251 (85)	247 (78)	280 (84)	0.25	0.62	0.006

Note: Data are presented as mean (±SD).

p < 0.05.

Abbreviations: FEV1, forced expiratory volume in 1 second; RV, residual volume; TLC, total lung capacity; FVC, forced vital capacity; TLC, total lung capacity; 6-MWT, 6-minute walking test; mMRC score, modified Medical Research Council score; Lt, liters; m, meters.

At 3 months after LVRC, 51 out of the 72 subjects (71%) met at least one PFT/6-MWT response criterion (Figure 2). The response outcome, Δ6-MWT ≥ 26 m, was met the most frequently in 55% (34/62) of the patients. The response rate for ΔFEV1 ≥ 12% was 32% (26/70) while the response rate for ΔRV ≥ 10% was 42% (29/69). A lobar volume reduction (LVR) ≥ 350 mL was met in 19% (14/72) of cases. Because of the poor response rate of LVR ≥ 350 mL, it was not included in the stepwise linear regression analysis. Furthermore, 16% of the patients met the 6-MWT as well as the RV response criterion and 10% of the patients met both the 6-MWT and FEV1 or the FEV1 and RV response criteria, respectively; 20% of the patients met all three aforementioned response criteria.

Figure 2

Treatment response rates for Δ6-MWT ≥ 26 m, ΔFEV1 ≥ 12%, and ΔRV ≥ 10%.

Abbreviations: FEV1, forced expiratory volume in 1 second; RV, residual volume; 6-MWT, 6-minute walking test.

The stepwise linear regression analysis was applied to the response criteria Δ6-MWT ≥ 26 m, ΔFEV1 ≥ 12%, and ΔRV ≥ 10% to find predictors for LVRC treatment. Each response criteria consisted of a PFT/6-MWT component as well as a QCT component; they were all found to be statistically significant (p < 0.05). The clinical predictors for Δ6-MWT ≥ 26 m, ΔFEV1 ≥ 12%, and ΔRV ≥ 10% were 6-MWT at baseline (p = 0.0003), FEV1 at baseline (p = 0.02), and TLC at baseline (p = 0.0014) respectively (Table 2; Figure 3). Thereby, the lower the 6-MWT at baseline, the greater the 6-MWT increase achieved; the lower the FEV1 at baseline, the greater the FEV1 improvement achieved; the higher the TLC at baseline, the greater the RV reduction achieved.

Table 2

Clinical and quantitative CT predictors of positive outcome to coils treatment

Positive outcome	Predictors	p-value	Adj R2
Δ6-MWT	6-MWT at baseline	0.0003	0.22
	Standard deviation of low attenuation cluster sizes in the peripheral region of treated lung	0.037
ΔFEV	FEV1 at baseline	0.02	0.15
	Median size of low attenuation clusters in central region of the target lobe	0.0018
ΔRV	TLC at baseline	0.0014	0.28
	Standard deviation of low attenuation cluster sizes in the peripheral region of the target lobe	0.007

Abbreviations: FEV1, forced expiratory volume in 1 second; RV, residual volume; TLC, total lung capacity; 6-MWT, 6-minute walking test.

Figure 3

(A) Box plot of responders to coils treatment looking at Δ6-MWT > 26 m. Larger standard deviation of LAC sizes in peripheral regions of treated lung also responded more frequently. (B) Box plot of responders to coils treatment looking at ΔFEV1 ≥ 12%. Larger median LAC sizes in the central regions of treated lobe also responded more frequently. (C) Box plot of responders to coils treatment looking at ΔRV ≥ 10%. Larger standard deviation of LAC sizes in peripheral regions of treated lobe also responded more frequently.

Abbreviations: FEV1, forced expiratory volume in 1 second; RV, residual volume; 6-MWT, 6-minute walking test; TLC, total lung capacity; LAC, low-attenuation cluster; Lt, liters; m, meters; cc, milliliters; SD, standard deviation.

The standard deviation of LAC sizes in the peripheral region of the treated lung was deemed a QCT predictor for Δ6-MWT ≥ 26 m (p = 0.037), whereby larger SD of LAC sizes in peripheral regions of treated lung responded more frequently. A QCT predictor for ΔFEV1 ≥ 12% was the median size of LAC in the central region of the target lobe (p = 0.0018), whereby larger median LAC sizes responded better to treatment. Lastly, the standard deviation of LAC size in the peripheral region of the target lobe (p = 0.007) was tested as a QCT predictor for RV reduction (Table 2; Figure 3). The larger the SD of LAC sizes in peripheral regions of the treated lung, the greater the RV reduction achieved. Other QCT parameters such as the EP, HS, and air trapping were found to not be predictors for positive outcome to LVRC treatment.

Discussion

We have observed variability in the clinical outcome amongst patients undergoing LVRC treatment in our hospital. Subject selection has previously relied on ad hoc criteria adapted from the NETT study.11 In this retrospective analysis, we assessed QCT measurements and possible clinical predictors of outcome with the aim of identifying optimal candidates and improving the treatment outcome.

Several single arm trials and three RCT evaluated the feasibility and efficacy of the LVRC treatment as minimally invasive therapeutic approach for patients with severe emphysema.9,10,24–28 The largest RCT is the RENEW (Effect of Endobronchial Coils Treatment vs Usual Care in Patients with Severe Emphysema) Trial, in that 315 emphysema patients were randomly assigned to a coil treatment group and to a standard medical care group.14 At 12 months, a statistically significant between-group difference in median change was achieved for the 6-MWT with 14.6 m (p = 0.02), for FEV1 with 7% (p < 0.001) and for SGRQ with −8.9 points (p < 0.001) each favoring the coil group. Although these results showed a statistically significant improvement in the clinical outcome measures, the between-group difference was modest and of uncertain clinical importance. To date, there are only limited data related to outcome predictors following LVRC treatment14,24 but identifying these predictors are crucial given the complexity, expense, and irreversibility of the treatment.

In this trial, we evaluated clinical and QCT outcome measures as potential predictors for the clinical outcome following coil treatment (Figure 4). Several studies have already evaluated the possible relationships among QCT and spirometric measurements of COPD severity,29 DLCO measurements, visual assessment of emphysema30 and physiological indices, eg, body-mass index (BMI).31 Furthermore, when comparing computerized evaluation to visual assessment, QCT is known to be less operator-dependent than visual grading of the severity of emphysema.32,33 QCT outcome predictors for good response to endoscopic valve therapy were previously reported,15 but so far, no QCT variables are known that predict the outcome following coil treatment.

Figure 4

Lung volume reduction responder with severe emphysema treated for the right upper lobe, successfully identified as a responder by quantitative computed tomography. (A) Low-attenuation cluster representation of the patient at baseline. The low-attenuation area percentage of the right upper lobe (RUL) was found to be [LAA%] − 950 = 40.41%. (B) Three-dimensional surface lung volume reduction representation demonstrating a reduction in RUL volume of 531.3 mL after treatment, corresponding to a reduction by one-third of the baseline volume. Baseline (left) shows an enlarged RUL while post-treatment (right) shows the reduction of volume of RUL, and the relative expansion of the right middle lobe and the right lower lobe.

We noticed that patients exhibiting specific baseline clinical and QCT characteristics responded more positively to LVRC treatment. Clinical parameters of lower 6-MWT, lower FEV1, and higher TLC at baseline displayed higher likelihood of response to LVRC at 3-month follow-up. This finding correlates with a previous study result that a lower initial performance in the 6-MWT may predict an improvement in exercise capacity.10 Similar results were shown for patients undergoing lung volume reduction surgery11 or endoscopic valve therapy.34 Furthermore, patients with a high baseline TLC exhibited superior treatment response. Surprisingly, RV was not found to be a statistically significant outcome predictor. However a high TLC is also an indicator for severe hyperinflation and the RENEW trial similarly reported that severe hyperinflation (RV > 225%) was also found to predict a superior outcome following LVRC treatment.14 It should be kept in mind though, that not only one parameter should be used in order to determine the clinical importance of the therapy, but rather a combination of various parameters (eg, FEV1, RV, 6-MWT) that reflect in total the clinical relevance of this treatment approach. In this study however, the number of the patients was too small to evaluate predictors for combined efficacy outcome parameters.

Our QCT analysis showed that the standard deviation of LAC sizes in the peripheral region of the treated lung and in the peripheral region of the target lobe were a QCT predictor for 6-MWT MCID and for RV MCID, respectively. Furthermore, the median size of LAC in the central region of the target lobe showed a predictive value for the FEV1 MCID. These findings could indicate the mechanism of action of the coils. It is already known, that the regional emphysema distribution is associated with pulmonary function.35 Thereby, the emphysema severity in the central region of the lung affects the pulmonary function more than the peripheral emphysematous changes, potentially explaining why the median size of LAC of the central region influences the FEV1 in this trial (Figure 5). Peripheral disease seems to correlate better with static or dynamic hyperinflation and exercise capacity. Our findings suggest that a more inhomogeneous emphysema in the peripheral regions of the lung – which is reflected by a high standard deviation of LAC – responds better to coil treatment. However, HS on a lobar level was not found to be an outcome predictor. In this trial, we excluded patients with homogeneous emphysema. The pilot trial of LVRC in 20107 showed that the efficacy favored patients with heterogeneous compared to homogeneous emphysema. Thus, the patients enrolled in our trial and treated between 2011 and 2015 had a heterogeneous emphysema distribution. The first trial that demonstrated the efficacy of coil therapy also in patients with homogeneous emphysema was published in 2014 by Klooster et al,26 while the first mention of efficacy of LVRC in a large cohort of patients with homogenous emphysema was in the recently published RENEW trial in 2016.14

Figure 5

Importance of distribution of low attenuation clusters: central disease affects more the FEV1 while the peripheral disease affects more the hyperinflation and the exercise capacity.

Abbreviations: FEV1, forced expiratory volume in 1 second; RV, residual volume; 6-MWT, 6-minute walking test.

A difficulty of identifying strong predictors of success has been previously demonstrated by Washko et al, in the outcome after lung volume reduction surgery in a large patient cohort of the NETT trial. The baseline burden of CT emphysema (LAA% less than −950 Hounsfield units) and the emphysema distribution (ratio upper to lower zone emphysema) were only weakly predictive of a subject’s change in FEV1 and exercise capacity after surgical intervention.36 Our study is in concordance with these results, since we have not been able to find any correlation between EP, HS or air trapping with the outcome prediction.

Limitations

One significant limitation of this trial is that all patients were treated only unilaterally. Recently, it has been suggested that bilateral treatment is more efficacious. Therefore, the predictive factors should also be evaluated for the bilateral treatment approach. Another limitation is the retrospective character of the study. However, its aim was to identify predictors of success in patients treated with coils. Because radiological and lung-functional outcomes were unknown prior to treatment, a prospective analysis was not possible. Furthermore, the lack of a control group and the unblinded character of this study cannot exclude the possibility of introducing placebo effects in the PFT and exercise parameters, although QCT is mainly resistant to this influence. Another limitation is the post-treatment QCT parameters might also be affected by the presence of the numerous coils: they induce metallic hardening artifacts on CT as well as a different amount of increased density adjacent to the coils themselves potentially affecting the measurement and quantification of the emphysema parameters.

Conclusion

Patients exhibiting specific baseline clinical and QCT characteristics responded more positively to LVRC treatment. Patients with lower 6-MWT, FEV1, and higher TLC at baseline displayed a tendency to respond better. Patients with higher standard deviations in LAC size in the peripheral region of the treated lung or treated lobe had superior treatment outcome to 6-MWT and RV. Patients with higher median size of LAC size in the central regions of the treated lobe also experienced a more positive FEV1 outcome. These findings emphasize the role of accurate patient selection in successful coil therapy. In this study different parameters were found to be statistically significant, independent positive predictors of LVRC therapy outcome. However, for each patient all the aforementioned parameters should be evaluated to increase the likelihood of positive outcome. Additional studies are needed to improve the understanding of the predictive factors of response to better select the responders to LVRC and to prove the benefits and impact of this new QCT approach for adoption in routine clinical practice. Only with more precise patient selection will coil therapy become established as a substantial treatment for patients with advanced emphysema.