Hong-Bo Xiao1, Yi-Shan Wang2, Zhi-Feng Luo3, Xiang-Yang Lu4. 1. College of Veterinary Medicine, Hunan Agricultural University, China; Hunan Province University Key Laboratory for Agricultural Biochemistry and Biotransformation, Hunan Agricultural University, China; Hunan Engineering Research Center of Veterinary Drug, China. Electronic address: xhbzhb@hunau.edu.cn. 2. College of Veterinary Medicine, Hunan Agricultural University, China. 3. Department of Basic Medicine, Xiangnan University, China. 4. Hunan Province University Key Laboratory for Agricultural Biochemistry and Biotransformation, Hunan Agricultural University, China; Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients, China.
Abstract
AIMS: Recent investigations have shown that dimethylarginine dimethylaminohydrolase (DDAH) may be related to sleep disorder. The present study was conducted to test the hypnotic effect of jujubosides from Semen Ziziphi Spinosae (SZSJ) on sleep deprivation and the mechanisms involved. MATERIALS AND METHODS: Forty rats were randomly divided into 4 groups (n = 10): two groups of sleep-deprived rats treated with SZSJ (10 or 30 mg/kg per day), sleep-deprived rats group, and control group. At the end of experiment, cerebral DDAH expression, cerebral asymmetric dimethylarginine (ADMA) level, sleep parameters, behavioral activities, and cerebral neurotransmitters level brain was examined. KEY FINDINGS: Six days after treatment, SZSJ treatment up-regulated cerebral expression of DDAH I and DDAH II concomitantly with elevated parameters of total sleep time, cerebral γ‑aminobutyric acid and 5‑hydroxytryptamine, and reduced values of forelimb lifting-up frequency and walking time, cerebral 8-isoprostane, ADMA, noradrenaline, and glutamic acid in sleep deprivation of rats. SIGNIFICANCE: The present results suggest that SZSJ Protects against insomnia by a decrease in ADMA level and an improvement in DDAH production in sleep-deprived rats.
AIMS: Recent investigations have shown that dimethylarginine dimethylaminohydrolase (DDAH) may be related to sleep disorder. The present study was conducted to test the hypnotic effect of jujubosides from Semen Ziziphi Spinosae (SZSJ) on sleep deprivation and the mechanisms involved. MATERIALS AND METHODS: Forty rats were randomly divided into 4 groups (n = 10): two groups of sleep-deprived rats treated with SZSJ (10 or 30 mg/kg per day), sleep-deprived rats group, and control group. At the end of experiment, cerebral DDAH expression, cerebral asymmetric dimethylarginine (ADMA) level, sleep parameters, behavioral activities, and cerebral neurotransmitters level brain was examined. KEY FINDINGS: Six days after treatment, SZSJ treatment up-regulated cerebral expression of DDAH I and DDAH II concomitantly with elevated parameters of total sleep time, cerebral γ‑aminobutyric acid and 5‑hydroxytryptamine, and reduced values of forelimb lifting-up frequency and walking time, cerebral 8-isoprostane, ADMA, noradrenaline, and glutamic acid in sleep deprivation of rats. SIGNIFICANCE: The present results suggest that SZSJ Protects against insomnia by a decrease in ADMA level and an improvement in DDAH production in sleep-deprived rats.
Authors: Varvara-Maria Chalioti; Vassilis G Giannakoulis; Eleni Papoutsi; Aikaterini Megalou; Konstantinos Kritikos; Panagiotis Konstantopoulos; Paraskevi Roussou; Konstantinos Toutouzas; Despina N Perrea Journal: J Family Med Prim Care Date: 2020-12-31