Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Detection of a subunit of cellular Pol II within highly purified preparations of RNA polymerase isolated from rabbit poxvirus virions.

Literature DB >> 3004740

Detection of a subunit of cellular Pol II within highly purified preparations of RNA polymerase isolated from rabbit poxvirus virions.

Abstract

A large (approximately 200 kd) subunit of cellular RNA polymerase II (Pol II) and a virus-encoded subunit of rabbit poxvirus (RPV) RNA polymerase (137 kd) react with common monoclonal antibodies. Hybridization studies with viral and cellular DNA clones confirm that the viral and cellular proteins are related. Following RPV infection, the Pol II subunit is translocated from the nucleus to the cytoplasmic virosomes, then packaged into mature virus, and is found associated with the viral RNA polymerase purified from virions. The results suggest that the cellular Pol II subunit may be directly involved in the transcription of viral genes.

Entities: Disease Species

Mesh：

Substances：

Year: 1986 PMID： 3004740 DOI： 10.1016/0092-8674(86)90268-0

Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582

Keyword Cloud
Cited

19 in total

1. Early transcription factor subunits are encoded by vaccinia virus late genes.

Authors: P D Gershon; B Moss
Journal: Proc Natl Acad Sci U S A Date: 1990-06 Impact factor: 11.205

2. Orthopoxvirus gene expression in Xenopus laevis oocytes: a component of the virion is needed for late gene expression.

Authors: R F Massung; R W Moyer
Journal: J Virol Date: 1990-05 Impact factor: 5.103

3. A vaccinia virus late transcription factor copurifies with a factor that binds to a viral late promoter and is complemented by extracts from uninfected HeLa cells.

Authors: C F Wright; A E Hubbs; S K Gunasinghe; B W Oswald
Journal: J Virol Date: 1998-02 Impact factor: 5.103

4. Sedimentation of an RNA polymerase complex from vaccinia virus that specifically initiates and terminates transcription.

Authors: S S Broyles; B Moss
Journal: Mol Cell Biol Date: 1987-01 Impact factor: 4.272

5. Antitumor activity and immunogenicity of recombinant vaccinia virus expressing HPV 16 E7 protein SigE7LAMP is enhanced by high-level coexpression of IGFBP-3.

Authors: J Musil; L Kutinova; K Zurkova; P Hainz; K Babiarova; J Krystofova; S Nemeckova
Journal: Cancer Gene Ther Date: 2014-02-21 Impact factor: 5.987

Detection of a subunit of cellular Pol II within highly purified preparations of RNA polymerase isolated from rabbit poxvirus virions.

1. Early transcription factor subunits are encoded by vaccinia virus late genes.

2. Orthopoxvirus gene expression in Xenopus laevis oocytes: a component of the virion is needed for late gene expression.

3. A vaccinia virus late transcription factor copurifies with a factor that binds to a viral late promoter and is complemented by extracts from uninfected HeLa cells.

4. Sedimentation of an RNA polymerase complex from vaccinia virus that specifically initiates and terminates transcription.

5. Antitumor activity and immunogenicity of recombinant vaccinia virus expressing HPV 16 E7 protein SigE7LAMP is enhanced by high-level coexpression of IGFBP-3.

6. Host cell nuclear proteins are recruited to cytoplasmic vaccinia virus replication complexes.

7. Association of non-viral proteins with recombinant vaccinia virus virions.

8. Attenuation of vaccinia virus by the expression of human Flt3 ligand.

9. A cellular factor is required for transcription of vaccinia viral intermediate-stage genes.

10. Early promoter-binding factor from vaccinia virions.