Literature DB >> 30047058

Transposon Sequencing of Vibrio cholerae in the Infant Rabbit Model of Cholera.

Lauren M Shull1, Andrew Camilli2.   

Abstract

Transposon sequencing, or Tn-seq, combines transposon mutagenesis and massively parallel sequencing to allow for rapid and high-throughput identification of genes that play roles in fitness within environments of interest. The bacterial pathogen Vibrio cholerae is an excellent candidate for Tn-seq screens due to the availability of a plasmid-based in vivo transposition system and the relative ease with which the cholera disease state can be modeled in animals. This chapter will describe a method for performing Tn-seq screens on V. cholerae in the infant rabbit model of cholera.

Entities:  

Keywords:  Cholera; Infant rabbit; Tn-seq; Transposon sequencing; Vibrio cholerae; Virulence

Mesh：

Substances：

Year:  2018        PMID： 30047058      PMCID： PMC7211033          DOI： 10.1007/978-1-4939-8685-9_10

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  10 in total

Review 1.  The suckling mouse model of cholera.

Authors:  K E Klose
Journal:  Trends Microbiol       Date:  2000-04       Impact factor: 17.079

2.  Tn10 insertion specificity is strongly dependent upon sequences immediately adjacent to the target-site consensus sequence.

Authors:  J Bender; N Kleckner
Journal:  Proc Natl Acad Sci U S A       Date:  1992-09-01       Impact factor: 11.205

3.  Identification of in vivo regulators of the Vibrio cholerae xds gene using a high-throughput genetic selection.

Authors:  Emilykate McDonough; David W Lazinski; Andrew Camilli
Journal:  Mol Microbiol       Date:  2014-03-14       Impact factor: 3.501

Review 4.  Transposon insertion sequencing: a new tool for systems-level analysis of microorganisms.

Authors:  Tim van Opijnen; Andrew Camilli
Journal:  Nat Rev Microbiol       Date:  2013-05-28       Impact factor: 60.633

5.  Back to the future: studying cholera pathogenesis using infant rabbits.

Authors:  Jennifer M Ritchie; Haopeng Rui; Roderick T Bronson; Matthew K Waldor
Journal:  MBio       Date:  2010-05-18       Impact factor: 7.867

6.  Gene fitness landscapes of Vibrio cholerae at important stages of its life cycle.

Authors:  Heather D Kamp; Bharathi Patimalla-Dipali; David W Lazinski; Faith Wallace-Gadsden; Andrew Camilli
Journal:  PLoS Pathog       Date:  2013-12-26       Impact factor: 6.823

7.  Tn-seq: high-throughput parallel sequencing for fitness and genetic interaction studies in microorganisms.

Authors:  Tim van Opijnen; Kip L Bodi; Andrew Camilli
Journal:  Nat Methods       Date:  2009-09-20       Impact factor: 28.547

8.  Homopolymer tail-mediated ligation PCR: a streamlined and highly efficient method for DNA cloning and library construction.

Authors:  David W Lazinski; Andrew Camilli
Journal:  Biotechniques       Date:  2013-01       Impact factor: 1.993

9.  Identification of essential genes of the periodontal pathogen Porphyromonas gingivalis.

Authors:  Brian A Klein; Elizabeth L Tenorio; David W Lazinski; Andrew Camilli; Margaret J Duncan; Linden T Hu
Journal:  BMC Genomics       Date:  2012-10-31       Impact factor: 3.969

Review 10.  Genome-Wide Fitness and Genetic Interactions Determined by Tn-seq, a High-Throughput Massively Parallel Sequencing Method for Microorganisms.

Authors:  Tim van Opijnen; David W Lazinski; Andrew Camilli
Journal:  Curr Protoc Mol Biol       Date:  2014-04-14
  10 in total
  2 in total

1.  Experimental evolution of Vibrio cholerae identifies hypervesiculation as a way to increase motility in the presence of polymyxin B.

Authors:  Sean Giacomucci; Annabelle Mathieu-Denoncourt; Antony T Vincent; Hanen Jannadi; Marylise Duperthuy
Journal:  Front Microbiol       Date:  2022-08-22       Impact factor: 6.064

2.  Acinetobacter baylyi regulates type IV pilus synthesis by employing two extension motors and a motor protein inhibitor.

Authors:  Courtney K Ellison; Triana N Dalia; Catherine A Klancher; Joshua W Shaevitz; Zemer Gitai; Ankur B Dalia
Journal:  Nat Commun       Date:  2021-06-18       Impact factor: 14.919
  2 in total

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