Jie Zhang1, Gui-Qiu Zhao1, Jing Qu2, Jing Lin1, Cheng-Ye Che1, Xue-Jiao Yang1. 1. Department of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong Province, China. 2. Department of Administrative Office, the Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong Province, China.
Abstract
AIM: To investigate the expression of pentraxin 3 (PTX3) in rat corneal epithelium at the early stage of Aspergillus fumigatus (A. fumigatus) infection. METHODS: A total of 50 Wistar rats were randomly divided into control group, Sham group and experimental group (fungal keratitis group, FK group). The right eye was chosen as the experiment one and infected by A. fumigatus. Rats were executed at 8, 16 and 24h after the experimental models being established. Corneal epithelia were collected to assess the expression of PTX3 by quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot analysis. RESULTS: Corneal inflammation scores increased as infection prolonged (P<0.05, P<0.001). PTX3 mRNA expression was low in normal and Sham group rats' corneas. Level of PTX3 mRNA in infected rat cornea was elevated at 8h and peaked at 16h. The difference was significant compared with control group (P<0.001). Western blot analysis also showed a significant increase of PTX3 protein in experimental group at 8h and peaked at 16h (P<0.001). The synchronous expression of control group and experimental group were also in significant difference (P<0.001). CONCLUSION: PTX3 exists in cornea epithelium and is significantly increased after A. fumigatus infection. PTX3 plays an important role in the early stage of cornea innate immunity against A. fumigatus.
AIM: To investigate the expression of pentraxin 3 (PTX3) in rat corneal epithelium at the early stage of Aspergillus fumigatus (A. fumigatus) infection. METHODS: A total of 50 Wistar rats were randomly divided into control group, Sham group and experimental group (fungal keratitis group, FK group). The right eye was chosen as the experiment one and infected by A. fumigatus. Rats were executed at 8, 16 and 24h after the experimental models being established. Corneal epithelia were collected to assess the expression of PTX3 by quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot analysis. RESULTS:Corneal inflammation scores increased as infection prolonged (P<0.05, P<0.001). PTX3 mRNA expression was low in normal and Sham group rats' corneas. Level of PTX3 mRNA in infected rat cornea was elevated at 8h and peaked at 16h. The difference was significant compared with control group (P<0.001). Western blot analysis also showed a significant increase of PTX3 protein in experimental group at 8h and peaked at 16h (P<0.001). The synchronous expression of control group and experimental group were also in significant difference (P<0.001). CONCLUSION:PTX3 exists in cornea epithelium and is significantly increased after A. fumigatusinfection. PTX3 plays an important role in the early stage of cornea innate immunity against A. fumigatus.
Authors: Bing Han; Marco Mura; Cristiano F Andrade; Daisuke Okutani; Monika Lodyga; Claudia C dos Santos; Shaf Keshavjee; Michael Matthay; Mingyao Liu Journal: J Immunol Date: 2005-12-15 Impact factor: 5.422
Authors: F Breviario; E M d'Aniello; J Golay; G Peri; B Bottazzi; A Bairoch; S Saccone; R Marzella; V Predazzi; M Rocchi Journal: J Biol Chem Date: 1992-11-05 Impact factor: 5.157