Kumar S D Kothapalli1, Hui Gyu Park2, Xiaoxian Guo3, Xuepeng Sun3, James Zou3, Stephanie S Hyon3, Xia Qin4, Peter Lawrence3, Rinat R Ran-Ressler3, Ji Yao Zhang3, Zhenglong Gu5, J Thomas Brenna6. 1. Division of Nutritional Sciences, Cornell University, Ithaca, NY, United States; Dell Pediatric Research Institute, Dell Medical School, The University of Texas at Austin, Austin, TX, United States. Electronic address: kkothapalli@utexas.edu. 2. Division of Nutritional Sciences, Cornell University, Ithaca, NY, United States; Dell Pediatric Research Institute, Dell Medical School, The University of Texas at Austin, Austin, TX, United States. 3. Division of Nutritional Sciences, Cornell University, Ithaca, NY, United States. 4. Division of Nutritional Sciences, Cornell University, Ithaca, NY, United States; College of Veterinary Medicine, Jilin University, Changchun, Jilin, China. 5. Division of Nutritional Sciences, Cornell University, Ithaca, NY, United States. Electronic address: zg27@cornell.edu. 6. Division of Nutritional Sciences, Cornell University, Ithaca, NY, United States; Dell Pediatric Research Institute, Dell Medical School, The University of Texas at Austin, Austin, TX, United States. Electronic address: tbrenna@utexas.edu.
Abstract
INTRODUCTION: The only known non-pharmacological means to alter long chain polyunsaturated fatty acid (LCPUFA) abundance in mammalian tissue is by altering substrate fatty acid ratios. Alternative mRNA splicing is increasingly recognized as a modulator of protein structure and function. Here we report identification of a novel alternative transcript (AT) of fatty acid desaturase 2 (FADS2) that inhibits production of omega-3 but not omega-6 LCPUFA, discovered during study of ATs in human milk fat globules (MFG). METHODS: Human breastmilk collected from a single donor was used to isolate MFG. An mRNA-sequencing library was constructed from the total RNA isolated from the MFG. The constructed library was sequenced using an Illumina HiSeq instrument operating in high output mode. Expression levels of evolutionary conserved FADSAT were measured using cDNA from MFG by semi-quantitative RT-PCR assay. RESULTS: RNA sequencing revealed >15,000 transcripts, including moderate expression of the FADS2 classical transcript (CS). A novel FADS2 alternative transcript (FADS2AT2) with 386 amino acids was discovered. When FADS2AT2 was transiently transfected into MCF7 cells stably expressing FADS2, delta-6 desaturation (D6D) of alpha-linolenic acid 18:3n-3 → 18:4n-3 was suppressed as were downstream products 20:4n-3 and 20:5n-3. In contrast, no significant effect on D6D of linoleic acid 18:2n-6 → 18:3n-6 or downstream products was observed. FADS2, FADS2AT1 and 5 out of 8 known FADS3AT were expressed in MFG. FADS1, FADS3AT3, and FADS3AT5 are undetectable. CONCLUSION: The novel, noncatalytic FADS2AT2 regulates FADS2CS-mediated Δ6-desaturation of omega-3 but not omega-6 PUFA biosynthesis. This spliced isoform mediated interaction is the first molecular mechanism by which desaturation of one PUFA family but not the other is modulated.
INTRODUCTION: The only known non-pharmacological means to alter long chainpolyunsaturated fatty acid (LCPUFA) abundance in mammalian tissue is by altering substrate fatty acid ratios. Alternative mRNA splicing is increasingly recognized as a modulator of protein structure and function. Here we report identification of a novel alternative transcript (AT) of fatty acid desaturase 2 (FADS2) that inhibits production of omega-3 but not omega-6 LCPUFA, discovered during study of ATs in humanmilk fat globules (MFG). METHODS:Human breastmilk collected from a single donor was used to isolate MFG. An mRNA-sequencing library was constructed from the total RNA isolated from the MFG. The constructed library was sequenced using an Illumina HiSeq instrument operating in high output mode. Expression levels of evolutionary conserved FADSAT were measured using cDNA from MFG by semi-quantitative RT-PCR assay. RESULTS: RNA sequencing revealed >15,000 transcripts, including moderate expression of the FADS2 classical transcript (CS). A novel FADS2 alternative transcript (FADS2AT2) with 386 amino acids was discovered. When FADS2AT2 was transiently transfected into MCF7 cells stably expressing FADS2, delta-6 desaturation (D6D) of alpha-linolenic acid 18:3n-3 → 18:4n-3 was suppressed as were downstream products 20:4n-3 and 20:5n-3. In contrast, no significant effect on D6D of linoleic acid 18:2n-6 → 18:3n-6 or downstream products was observed. FADS2, FADS2AT1 and 5 out of 8 known FADS3AT were expressed in MFG. FADS1, FADS3AT3, and FADS3AT5 are undetectable. CONCLUSION: The novel, noncatalytic FADS2AT2 regulates FADS2CS-mediated Δ6-desaturation of omega-3 but not omega-6 PUFA biosynthesis. This spliced isoform mediated interaction is the first molecular mechanism by which desaturation of one PUFA family but not the other is modulated.
Authors: Woo Jung Park; Kumar S D Kothapalli; Holly T Reardon; Peter Lawrence; Shu-Bing Qian; J Thomas Brenna Journal: J Lipid Res Date: 2012-05-22 Impact factor: 5.922
Authors: Elisabet Ognedal Berge; Vidar Staalesen; Anne Hege Straume; Johan Richard Lillehaug; Per Eystein Lønning Journal: Biochim Biophys Acta Date: 2010-01-15
Authors: Holly T Reardon; Andrea T Hsieh; Woo Jung Park; Kumar S D Kothapalli; Joshua C Anthony; Peter W Nathanielsz; J Thomas Brenna Journal: Prostaglandins Leukot Essent Fatty Acids Date: 2012-03-06 Impact factor: 4.006