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Literature DB >> 300413

A microassay for antibody binding to tumor cell surface antigens using 125I-labelled protein a from Staphylococcus aureus.

Abstract

A sensitive assay for antibodies bound to the surface antigens of adherent tumor cells using protein A from Staphylococcus aureus is described. Cells, as monolayers in microtest wells, are incubated first with immune or control serum, and then with 125I-labelled protein A (IPA), which binds to IgG antibodies bound to cell surface antigens. The IPA assay is shown to be more sensitive than the conventional isotopic antiglobulin (IAG) assay, because IPA binds to a lesser extent to IgG bound non-specifically to cells.

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Year: 1977 PMID： 300413 DOI： 10.1016/0022-1759(77)90017-5

Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303

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Cited

13 in total

1. Antibody response of mice to chemically induced tumors.

Authors: J P Brown; J M Klitzman; I Hellström; R C Nowinski; K E Hellström
Journal: Proc Natl Acad Sci U S A Date: 1978-02 Impact factor: 11.205

2. Immunoselection of tumor variants resistant to antibody-mediated cytotoxicity. Their immunologic and metastatic characterization.

Authors: J R Starkey; W C Davis; J E Talmadge
Journal: Cancer Immunol Immunother Date: 1982 Impact factor: 6.968

3. Gene dosage and gene expression in the HLA region: evidence from deletion variants.

Authors: P Gladstone; L Fueresz; D Pious
Journal: Proc Natl Acad Sci U S A Date: 1982-02 Impact factor: 11.205

4. Antibodies direct against the pancreatic islet cell plasma membrane. Detection and specificity.

Authors: A Lernmark; T Kanatsuna; C Patzelt; K Diakoumis; R Carroll; A H Rubenstein; D F Steiner
Journal: Diabetologia Date: 1980-11 Impact factor: 10.122

5. Activity of antibodies recovered from immune complexes of ovarian cancer patients.

Authors: P M Lutz; J R Dawson
Journal: Cancer Immunol Immunother Date: 1984 Impact factor: 6.968

6. A monoclonal antibody with binding and inhibiting activity towards human pancreatic carcinoma cells. I. Immunohistological and immunochemical characterization of a murine monoclonal antibody selecting for well differentiated adenocarcinomas of the pancreas.

Authors: K Bosslet; H F Kern; E J Kanzy; A Steinstraesser; A Schwarz; G Lüben; H U Schorlemmer; H H Sedlacek
Journal: Cancer Immunol Immunother Date: 1986 Impact factor: 6.968

A microassay for antibody binding to tumor cell surface antigens using 125I-labelled protein a from Staphylococcus aureus.

1. Antibody response of mice to chemically induced tumors.

2. Immunoselection of tumor variants resistant to antibody-mediated cytotoxicity. Their immunologic and metastatic characterization.

3. Gene dosage and gene expression in the HLA region: evidence from deletion variants.

4. Antibodies direct against the pancreatic islet cell plasma membrane. Detection and specificity.

5. Activity of antibodies recovered from immune complexes of ovarian cancer patients.

6. A monoclonal antibody with binding and inhibiting activity towards human pancreatic carcinoma cells. I. Immunohistological and immunochemical characterization of a murine monoclonal antibody selecting for well differentiated adenocarcinomas of the pancreas.

7. Detection of a recombinant murine leukemia virus-related glycoprotein on virus-negative thymoma cells.

8. Serum antibodies against alcohol-treated rabbit hepatocytes in patients with alcoholic liver disease.

9. Histocompatibility antigens on astrocytoma cells.

10. Identification of a cell surface protein, p97, in human melanomas and certain other neoplasms.