Structural characterization and antitumor effects of fucoidans from brown algae Kjellmaniella crassifolia farmed in northern China.

Brown alga-derived fucoidan has been proven to have a variety of bioactivities. To explore the antitumor effect of fucoidan, Kjellmaniella crassifolia (farmed in Dalian, China)was enzymatically digested to obtain the crude extract (F), which was further separated into three fractions (F1, F2 and F3). The monosaccharide composition and structural characteristics of the isolated fractions were determined using high-performance liquid chromatography (HPLC), Fourier-transform infrared spectroscopy (FTIR) and 1D and 2D nuclear magnetic resonance (NMR) spectroscopy. F1 is an acetylated galactofucan, and F2 consists of fucose, galactose, mannose and glucuronic acid. F3 has two components, an acetylated galactofucan and a pure sulfated fucan. F, F1 and F2 showed limited cytotoxicity against murine hepatocarcinoma Hca-F cells in vitro. Oral administration of F at a dose of 450 mg/kg d significantly inhibited lump growth in Hca-F-inoculated mice and led to upregulated FAS expression in tumor tissues compared to that of the control. F1 and F2 did not show competitive antineoplastic efficacy, as did the crude extract. Crude fucoidan could be a promising antitumor adjuvant. The origin of its efficacy may be the small molecules, such as phenols that attached to native fucoidan. This theory needs to be further confirmed.