XuanJi Li1, Christopher Rensing2,3, William L Taylor4, Caitlin Costelle4, Asker Daniel Brejnrod1, Robert J Ferry5, Paul B Higgins6, Franco Folli7, Kameswara Rao Kottapalli8, Gene B Hubbard9, Edward J Dick6, Shibu Yooseph3,10, Karen E Nelson3, Natalia Schlabritz-Loutsevitch11. 1. Department of Biology, University of Copenhagen, Universitetsparken, Denmark. 2. Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, China. 3. J. Craig Venter Institute, La Jolla, California. 4. Molecular Resource Center, University of Tennessee Health Science Center, Memphis, Tennessee. 5. Psychology Department, University of Memphis, Memphis, Tennessee. 6. Texas Biomedical Research Institute, San Antonio, Texas. 7. Endocrinology and Metabolism, Department of Health Science, University of Milano and ASST Santi Paolo e Carlo, Milano, Italy. 8. Center for Biotechnology and Genomics, Texas Tech University, Lubbock, Texas. 9. University of Texas Health Sciences Center at San Antonio, San Antonio, Texas. 10. Department of Computer Science, University of Central Florida, Orlando, Florida. 11. Department of Obstetrics and Gynecology, College of Medicine, Texas Tech University Health Sciences Center at the Permian Basin, Odessa, Texas.
Abstract
INTRODUCTION: Gut microbial communities are critical players in the pathogenesis of obesity. Pregnancy is associated with increased bacterial load and changes in gut bacterial diversity. Sparse data exist regarding composition of gut microbial communities in obesity combined with pregnancy. MATERIAL AND METHODS: Banked tissues were collected under sterile conditions during necropsy, from three non-obese (nOb) and four obese (Ob) near-term pregnant baboons. Sequences were assigned taxonomy using the Ribosomal Database Project classifier. Microbiome abundance and its difference between distinct groups were assessed by a nonparametric test. RESULTS: Three families predominated in both the nOb and Ob colonic microbiome: Prevotellaceae (25.98% and 32.71% respectively), Ruminococcaceae (12.96% and 7.48%), and Lachnospiraceae (8.78% and 11.74%). Seven families of the colon microbiome displayed differences between Ob and nOb groups. CONCLUSION: Changes in gut microbiome in pregnant obese animals open the venue for dietary manipulation in pregnancy.
INTRODUCTION: Gut microbial communities are critical players in the pathogenesis of obesity. Pregnancy is associated with increased bacterial load and changes in gut bacterial diversity. Sparse data exist regarding composition of gut microbial communities in obesity combined with pregnancy. MATERIAL AND METHODS: Banked tissues were collected under sterile conditions during necropsy, from three non-obese (nOb) and four obese (Ob) near-term pregnant baboons. Sequences were assigned taxonomy using the Ribosomal Database Project classifier. Microbiome abundance and its difference between distinct groups were assessed by a nonparametric test. RESULTS: Three families predominated in both the nOb and Ob colonic microbiome: Prevotellaceae (25.98% and 32.71% respectively), Ruminococcaceae (12.96% and 7.48%), and Lachnospiraceae (8.78% and 11.74%). Seven families of the colon microbiome displayed differences between Ob and nOb groups. CONCLUSION: Changes in gut microbiome in pregnant obese animals open the venue for dietary manipulation in pregnancy.
Authors: D Farley; M E Tejero; A G Comuzzie; P B Higgins; L Cox; S L Werner; S L Jenkins; C Li; J Choi; E J Dick; G B Hubbard; P Frost; D J Dudley; B Ballesteros; G Wu; P W Nathanielsz; N E Schlabritz-Loutsevitch Journal: Placenta Date: 2009-07-25 Impact factor: 3.481
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