Ana Cláudia Raposo1, Ricardo Dias Portela2, Ali Masmali3, Vinicius Cardoso-Brito1, Marcos Bernardo2, Dayse Cury Oliveira4, Arianne Pontes Oriá1. 1. School of Veterinary Medicine and Zootechny, Federal University of Bahia, Salvador, BA, Brazil. 2. Institute of Health Sciences, Federal University of Bahia, Salvador, BA, Brazil. 3. Department of Optometry, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi Arabia. 4. Brazilian Institute of Ophthalmology and Prevention of Blindness, Bahia School of Medicine and Public Health, Salvador, BA, Brazil.
Abstract
BACKGROUND: To evaluate the composition and characteristics of capuchin monkey (CM) tear film. METHODS: Eleven CM (Sapajus sp.) was evaluated. Strip meniscometry test (SMT), osmolarity, and tear ferning test (TFT) (by Rolando and Masmali scales) were assessed. Tear protein profile (SDS-PAGE), and total protein, albumin, urea, glucose, and cholesterol levels in tear film and blood serum were screened. RESULTS: Median ± semi-interquatil range for SMT and osmolarity values were 8.0 ± 1.625 and 303.0 ± 9.875, respectively. TFT for Rolando was 2.0 ± 0.5, and Masmali was 2.0 ± 0.0. Monkeys's tear obtained type II and III for Rolando, and 1 and 2 for Masmali. Tear components showed protein bands among 23-217 kDa, and presence of albumin, urea, glucose, and cholesterol. CONCLUSIONS: The results of SMT, osmolarity, TFT, SDS-PAGE, and tear biochemistry may serve as a reference baseline for CM, and the data may serve as a basis for future experimental model evaluations.
BACKGROUND: To evaluate the composition and characteristics of capuchin monkey (CM) tear film. METHODS: Eleven CM (Sapajus sp.) was evaluated. Strip meniscometry test (SMT), osmolarity, and tear ferning test (TFT) (by Rolando and Masmali scales) were assessed. Tear protein profile (SDS-PAGE), and total protein, albumin, urea, glucose, and cholesterol levels in tear film and blood serum were screened. RESULTS: Median ± semi-interquatil range for SMT and osmolarity values were 8.0 ± 1.625 and 303.0 ± 9.875, respectively. TFT for Rolando was 2.0 ± 0.5, and Masmali was 2.0 ± 0.0. Monkeys's tear obtained type II and III for Rolando, and 1 and 2 for Masmali. Tear components showed protein bands among 23-217 kDa, and presence of albumin, urea, glucose, and cholesterol. CONCLUSIONS: The results of SMT, osmolarity, TFT, SDS-PAGE, and tear biochemistry may serve as a reference baseline for CM, and the data may serve as a basis for future experimental model evaluations.
Authors: Paula Elisa Brandão Guedes; Jéssica Fontes Veloso; Luciana Carvalho Lacerda; Juliano Oliveira Santana; Irma Yuliana Mora-Ocampo; Carlos Priminho Pirovani; Rebeca Dalety Santos Cruz; Alexandre Dias Munhoz; Renata Santiago Alberto Carlos Journal: BMC Vet Res Date: 2021-12-14 Impact factor: 2.741
Authors: Jéssica Fontes Veloso; Paula Elisa Brandão Guedes; Luciana Carvalho Lacerda; Juliano Oliveira Santana; Irma Yuliana Mora-Ocampo; Carlos Priminho Pirovani; Arianne Pontes Oriá; Alexandre Dias Munhoz; Renata Santiago Alberto Carlos Journal: Vet Med Int Date: 2021-07-15