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Literature DB >> 3003738

Involvement of bases 787-795 of Escherichia coli 16S ribosomal RNA in ribosomal subunit association.

Abstract

A nine-base DNA oligomer [d(GTATCTAAT)] was used to probe the accessibility and function of bases in the region 787-795 of Escherichia coli 16S rRNA. Hybridization of the cDNA [d(GTATCTAAT)] to 16S rRNA in situ was carried out by binding the probe to intact 30S ribosomal subunits. Nitrocellulose filter binding showed that cDNA hybridization saturated with increasing probe concentration, suggesting that the probe was binding to a discrete site or sites. RNase H digestion of the rRNA under the DNA . rRNA hybrid and sequencing of the resultant RNA fragments verified that the cDNA probe bound specifically to the 787-795 region. Hybridization experiments using the cDNA probe showed that bases in the 787-795 region of 16S rRNA are exposed on the surface of 30S subunits. The functional role of bases 787-795 was then tested by assaying various ribosomal activities with the cDNA in place. Results of these functional assays demonstrate that this 16S rRNA region is directly involved in the association of 30S and 50S subunits.

Entities: Gene Species

Mesh：

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Year: 1986 PMID： 3003738 PMCID： PMC322902 DOI： 10.1073/pnas.83.3.556

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

24 in total

1. Protection of specific sites in 23 S and 5 S RNA from chemical modification by association of 30 S and 50 S ribosomes.

Authors: W Herr; H F Noller
Journal: J Mol Biol Date: 1979-06-05 Impact factor: 5.469

2. Mechanism of ribosomal subunit association: discrimination of specific sites in 16 S RNA essential for association activity.

Authors: W Herr; N M Chapman; H F Noller
Journal: J Mol Biol Date: 1979-06-05 Impact factor: 5.469

3. 5'-32P labeling of RNA and DNA restriction fragments.

Authors: G Chaconas; J H van de Sande
Journal: Methods Enzymol Date: 1980 Impact factor: 1.600

4. Comparison of Escherichia coli tRNAPhe in the free state, in the ternary complex and in the ribosomal A and P sites by chemical probing.

Authors: S Douthwaite; R A Garrett; R Wagner
Journal: Eur J Biochem Date: 1983-03-15

5. An enzymatic approach for localization of oligodeoxyribonucleotide binding sites on RNA. Application to studying rRNA topography.

Authors: A S Mankin; E A Skripkin; N V Chichkova; A M Kopylov; A A Bogdanov
Journal: FEBS Lett Date: 1981-08-31 Impact factor: 4.124

6. Protection of ribosomal RNA from kethoxal in polyribosomes. Implication of specific sites in ribosome function.

Authors: D A Brow; H F Noller
Journal: J Mol Biol Date: 1983-01-05 Impact factor: 5.469

7. Sequencing end-labeled DNA with base-specific chemical cleavages.

Authors: A M Maxam; W Gilbert
Journal: Methods Enzymol Date: 1980 Impact factor: 1.600

8. Physical characteristics of the reconstitution intermediates (RI30 and RI30*) from the 30S ribosomal subunit of Escherichia coli.

Authors: M F Tam; W E Hill
Journal: Biochemistry Date: 1981-10-27 Impact factor: 3.162

9. Chemical probes for higher-order structure in RNA.

Authors: D A Peattie; W Gilbert
Journal: Proc Natl Acad Sci U S A Date: 1980-08 Impact factor: 11.205

10. Basepairing potential of the 3' terminus of 16S RNA: dependence on the functional state of the 30S subunit and the presence of protein S21.

Authors: C Backendorf; C J Ravensbergen; J Van der Plas; J H van Boom; G Veeneman; J Van Duin
Journal: Nucleic Acids Res Date: 1981-03-25 Impact factor: 16.971

17 in total

1. The small ribosomal subunit from Thermus thermophilus at 4.5 A resolution: pattern fittings and the identification of a functional site.

Authors: A Tocilj; F Schlünzen; D Janell; M Glühmann; H A Hansen; J Harms; A Bashan; H Bartels; I Agmon; F Franceschi; A Yonath
Journal: Proc Natl Acad Sci U S A Date: 1999-12-07 Impact factor: 11.205

9. Identification of defined sequences in domain V of E. coli 23S rRNA in the 50S subunit accessible for hybridization with complementary oligodeoxyribonucleotides.

Authors: R T Marconi; W E Hill
Journal: Nucleic Acids Res Date: 1988-02-25 Impact factor: 16.971

10. Mutation at position 791 in Escherichia coli 16S ribosomal RNA affects processes involved in the initiation of protein synthesis.

Authors: W E Tapprich; D J Goss; A E Dahlberg
Journal: Proc Natl Acad Sci U S A Date: 1989-07 Impact factor: 11.205

Involvement of bases 787-795 of Escherichia coli 16S ribosomal RNA in ribosomal subunit association.

1. Protection of specific sites in 23 S and 5 S RNA from chemical modification by association of 30 S and 50 S ribosomes.

2. Mechanism of ribosomal subunit association: discrimination of specific sites in 16 S RNA essential for association activity.

3. 5'-32P labeling of RNA and DNA restriction fragments.

4. Comparison of Escherichia coli tRNAPhe in the free state, in the ternary complex and in the ribosomal A and P sites by chemical probing.

5. An enzymatic approach for localization of oligodeoxyribonucleotide binding sites on RNA. Application to studying rRNA topography.

6. Protection of ribosomal RNA from kethoxal in polyribosomes. Implication of specific sites in ribosome function.

7. Sequencing end-labeled DNA with base-specific chemical cleavages.

8. Physical characteristics of the reconstitution intermediates (RI30 and RI30*) from the 30S ribosomal subunit of Escherichia coli.

9. Chemical probes for higher-order structure in RNA.

10. Basepairing potential of the 3' terminus of 16S RNA: dependence on the functional state of the 30S subunit and the presence of protein S21.

1. The small ribosomal subunit from Thermus thermophilus at 4.5 A resolution: pattern fittings and the identification of a functional site.

2. Improved statistical methods reveal direct interactions between 16S and 23S rRNA.

3. Crystal structures of complexes of the small ribosomal subunit with tetracycline, edeine and IF3.

4. Positions in the 30S ribosomal subunit proximal to the 790 loop as determined by phenanthroline cleavage.

5. 16S rRNA sequences of uncultivated hot spring cyanobacterial mat inhabitants retrieved as randomly primed cDNA.

6. A quantitative map of nucleotide substitution rates in bacterial rRNA.

7. Base changes at position 792 of Escherichia coli 16S rRNA affect assembly of 70S ribosomes.

8. Probing the alpha-sarcin region of Escherichia coli 23S rRNA with a cDNA oligomer.

9. Identification of defined sequences in domain V of E. coli 23S rRNA in the 50S subunit accessible for hybridization with complementary oligodeoxyribonucleotides.

10. Mutation at position 791 in Escherichia coli 16S ribosomal RNA affects processes involved in the initiation of protein synthesis.