Muhammet Fatih Kilinc1, Omer Gokhan Doluoglu2, Pinar Eylem Eser3, Yildiray Yildiz2, Veli Mert Yazar2, Ali Ayyildiz2, Sema Hucumenoglu3. 1. Department of Urology, Medical Science University, Ankara Training and Research Hospital, Ankara, Turkey. Electronic address: mdfatihkilinc@yahoo.com. 2. Department of Urology, Medical Science University, Ankara Training and Research Hospital, Ankara, Turkey. 3. Department of Pathology, Medical Science University, Ankara Training and Research Hospital, Ankara, Turkey.
Abstract
OBJECTIVE: To determine the effects of intraurethral erythropoietin (EPO) on an experimentally induced urethral injury in a rat model with respect to wound healing enhancement and the prevention of spongiofibrosis MATERIAL AND METHODS: A urethral injury model was created by traumatizing the urethra of male rats with a tilted-tip insulin injector. Thirty rats were randomly separated into 3 groups of 10; Group 1 (control) received 0.9% saline solution twice a day, Group II received EPO 25 IU/kg once a day and 0.9% saline solution once a day, and Group III received EPO 25 IU/kg twice a day. All applications were made intraurethrally via a 24 ga catheter sheath. To investigate inflammation and spongiofibrosis and congestion of vessels in the lamina propria, the penises of the rats were harvested for histopathologic evaluation after a follow-up period of 14 days. RESULTS: Histopathologic analysis revealed less fibrosis and inflammation and higher congestion of vessels in Group III that had received high-dose EPO. There was a significant decrease in both spongiofibrosis and inflammation and an increase in congestion in Groups II and III compared to the control group (P = .001, for all). In the comparison of Group II with Group III, no statistically significant differences were found in terms of these 3 parameters (P = .5, P = .6, P = .27, respectively). CONCLUSION: The results of this study have shown that EPO has a preventive effect on spongiofibrosis and improve urethral wound healing in a rat model of urethral injury.
OBJECTIVE: To determine the effects of intraurethral erythropoietin (EPO) on an experimentally induced urethral injury in a rat model with respect to wound healing enhancement and the prevention of spongiofibrosis MATERIAL AND METHODS: A urethral injury model was created by traumatizing the urethra of male rats with a tilted-tip insulin injector. Thirty rats were randomly separated into 3 groups of 10; Group 1 (control) received 0.9% saline solution twice a day, Group II received EPO 25 IU/kg once a day and 0.9% saline solution once a day, and Group III received EPO 25 IU/kg twice a day. All applications were made intraurethrally via a 24 ga catheter sheath. To investigate inflammation and spongiofibrosis and congestion of vessels in the lamina propria, the penises of the rats were harvested for histopathologic evaluation after a follow-up period of 14 days. RESULTS: Histopathologic analysis revealed less fibrosis and inflammation and higher congestion of vessels in Group III that had received high-dose EPO. There was a significant decrease in both spongiofibrosis and inflammation and an increase in congestion in Groups II and III compared to the control group (P = .001, for all). In the comparison of Group II with Group III, no statistically significant differences were found in terms of these 3 parameters (P = .5, P = .6, P = .27, respectively). CONCLUSION: The results of this study have shown that EPO has a preventive effect on spongiofibrosis and improve urethral wound healing in a rat model of urethral injury.
Authors: Eliseo Portilla-de Buen; Juan Pablo Ramirez-Contreras; Jonathan Matias Chejfec-Ciociano; Rodrigo Lopez-Falcony; David Garcia-Martinez; Jose Gonzalo Vazquez-Camacho; Clotilde Fuentes-Orozco; Francisco Jose Barbosa-Camacho; Juan Carlos Ibarrola-Peña; Alejandro Gonzalez-Ojeda Journal: Res Rep Urol Date: 2021-05-14