Yawei Shi1, Yang Zhao2, Yunjian Zhang1, NiJiati AiErken3, Nan Shao1, Runyi Ye1, Ying Lin4, Shenming Wang1. 1. The Department of Breast and Thyroid Surgery, The First Affiliated Hospital of Sun Yat-sen University, 58# Zhongshan Two Road, Guangzhou, Guangdong 510080, China. 2. The Department of Vascular Surgery, The Third Affiliated Hospital of Sun Yat-sen University, 600# Tianhe Road, Guangzhou, Guangdong 510000, China. 3. The Department of General Surgery, The Seventh Affiliated Hospital of Sun Yat-sen University, 628# Zhenyuan Road, Shenzhen, Guangdong 518100, China. 4. The Department of Breast and Thyroid Surgery, The First Affiliated Hospital of Sun Yat-sen University, 58# Zhongshan Two Road, Guangzhou, Guangdong 510080, China. Electronic address: 604793522@qq.com.
Abstract
AIMS: Breast cancer is the major diagnosed cancer and the leading reason of cancer related death among women, and the tumor size is one of the risk factors. Therefore, it is significant to reveal the principle of breaking the subtle homeostasis of cell cycle and sustaining chronic proliferation of cancer cells. MAIN METHODS: The expression of TNNT1 was examined by qPCR and western blotting. The effect of TNNT1 on cell proliferation was detected by MTT, colony formation and anchorage-independent growth assay. The percent of cells in different cell phase was analyzed by Flow cytometry. The mRNA and protein expression of genes involved in G1/S transition was assayed using qPCR and western blotting, respectively. KEY FINDINGS: The results showed that TNNT1 expression is significantly increased in breast cancer tissues and closely correlated with clinical stage, T and N classification. Further experiments demonstrate that TNNT1 contributes to proliferation of breast cells by promoting G1/S transition. SIGNIFICANCE: Our results extend the mechanisms of controlling cell cycle and may provide a novel therapeutic target to therapy breast cancer.
AIMS: Breast cancer is the major diagnosed cancer and the leading reason of cancer related death among women, and the tumor size is one of the risk factors. Therefore, it is significant to reveal the principle of breaking the subtle homeostasis of cell cycle and sustaining chronic proliferation of cancer cells. MAIN METHODS: The expression of TNNT1 was examined by qPCR and western blotting. The effect of TNNT1 on cell proliferation was detected by MTT, colony formation and anchorage-independent growth assay. The percent of cells in different cell phase was analyzed by Flow cytometry. The mRNA and protein expression of genes involved in G1/S transition was assayed using qPCR and western blotting, respectively. KEY FINDINGS: The results showed that TNNT1 expression is significantly increased in breast cancer tissues and closely correlated with clinical stage, T and N classification. Further experiments demonstrate that TNNT1 contributes to proliferation of breast cells by promoting G1/S transition. SIGNIFICANCE: Our results extend the mechanisms of controlling cell cycle and may provide a novel therapeutic target to therapy breast cancer.
Authors: Nicholas A Vitanza; Matt C Biery; Carrie Myers; Eric Ferguson; Ye Zheng; Emily J Girard; Justyna M Przystal; Giulia Park; Alyssa Noll; Fiona Pakiam; Conrad A Winter; Shelli M Morris; Jay Sarthy; Bonnie L Cole; Sarah E S Leary; Courtney Crane; Nicole A P Lieberman; Sabine Mueller; Javad Nazarian; Raphael Gottardo; Mi-Youn Brusniak; Andrew J Mhyre; James M Olson Journal: Neuro Oncol Date: 2021-03-25 Impact factor: 12.300