Literature DB >> 3003066

The binding of the chromosomal protein HMG-2a to DNA regions of reduced stabilities.

J W Brown, J A Anderson.   

Abstract

The interaction of immunopurified high mobility group 2a protein (HMG-2a) with DNA was examined by the nitrocellulose filter binding assay. The relative binding activity of HMG-2a for synthetic polynucleotides was: (dI).(dC) greater than (dA-dT).(dA-dT) greater than (dA).(dT) much greater than (dG).(dC) greater than (dG-dC).(dG-dC). The protein also exhibited a marked preference for (A + T)-rich restriction fragments derived from rat and Drosophila satellites, yeast centromeres, phage lambda, and the ovalbumin gene and its 5' flanking sequences. These preferential DNA interactions occurred at ionic strengths and temperatures within the physiological range which argue for an in vivo role of DNA stability in dictating the genomic distribution of the large Mr HMG proteins.

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Year:  1986        PMID： 3003066

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  15 in total

1.  DNA binding by single HMG box model proteins.

Authors:  H Xin; S Taudte; N R Kallenbach; M P Limbach; R S Zitomer
Journal:  Nucleic Acids Res       Date:  2000-10-15       Impact factor: 16.971

2.  Isolation and characterization of maize cDNAs encoding a high mobility group protein displaying a HMG-box.

Authors:  K D Grasser; G Feix
Journal:  Nucleic Acids Res       Date:  1991-05-25       Impact factor: 16.971

3.  Complex formation of nuclear proteins with the RNA polymerase I promoter and repeated elements in the external transcribed spacer of Cucumis sativus ribosomal DNA.

Authors:  U Zentgraf; V Hemleben
Journal:  Nucleic Acids Res       Date:  1992-07-25       Impact factor: 16.971

4.  Multiple proteins bind to the P2 promoter region of the zein gene pMS1 of maize.

Authors:  U G Maier; K D Grasser; M M Haass; G Feix
Journal:  Mol Gen Genet       Date:  1990-04

5.  High mobility group proteins cHMG1a, cHMG1b, and cHMGI are distinctly distributed in chromosomes and differentially expressed during ecdysone dependent cell differentiation.

Authors:  S Ghidelli; P Claus; G Thies; J R Wiśniewski
Journal:  Chromosoma       Date:  1997-04       Impact factor: 4.316

6.  Binding of protein uH2A and histone H2A to DNA.

Authors:  B Kirdar; N Dalay; E Bermek
Journal:  Cell Biophys       Date:  1989-02

7.  High mobility group proteins 1 and 2 stimulate binding of a specific transcription factor to the adenovirus major late promoter.

Authors:  F Watt; P L Molloy
Journal:  Nucleic Acids Res       Date:  1988-02-25       Impact factor: 16.971

Review 8.  DNA-protein interactions and spatial organization of DNA.

Authors:  W A Krajewski; S V Razin
Journal:  Mol Biol Rep       Date:  1993-10       Impact factor: 2.316

9.  DNA looping by the HMG-box domains of HMG1 and modulation of DNA binding by the acidic C-terminal domain.

Authors:  M Stros; J Stokrová; J O Thomas
Journal:  Nucleic Acids Res       Date:  1994-03-25       Impact factor: 16.971

10.  Nuclear proteins interact with RNA polymerase I promoter and repeated elements of the 5' external transcribed spacer of the rDNA of cucumber in a single-stranded stage.

Authors:  U Zentgraf; V Hemleben
Journal:  Plant Mol Biol       Date:  1993-09       Impact factor: 4.076
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