Literature DB >> 300306

C3-reacted sepharose: a preparative method for separating T and B lymphocytes.

P Casali, B M Perussia.   

Abstract

Lymphocytes bearing complement receptors were separated from lymphocyte suspensions using C3-reacted Sepharose columns as solid phase immunoabsorbents. The retained CR+ lymphocytes were recovered by elution of the column with IgG fraction purified from a rabbit antiserum to human C3. This paper describes the method, its specificity and the yields of cells obtained.

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Year:  1977        PMID: 300306      PMCID: PMC1540909     

Source DB:  PubMed          Journal:  Clin Exp Immunol        ISSN: 0009-9104            Impact factor:   4.330


  12 in total

1.  Cellular immunoadsorbents: a simplified technique for separation of lymphoid cell populations.

Authors:  E Kedar; M Ortiz de Landazuri; B Bonavida
Journal:  J Immunol       Date:  1974-03       Impact factor: 5.422

2.  The dynamic state of the lymphocyte membrane. Factors affecting the distribution and turnover of surface immunoglobulins.

Authors:  F Loor; L Forni; B Pernis
Journal:  Eur J Immunol       Date:  1972-06       Impact factor: 5.532

3.  Specific purification of lymphocyte populations on a digestible immunoabsorbent.

Authors:  S F Schlossman; L Hudson
Journal:  J Immunol       Date:  1973-01       Impact factor: 5.422

4.  Human peripheral lymphocytes bearing both B-cell complement receptors and T-cell characteristics for sheep erythrocytes detected by a mixed rosette method.

Authors:  J W Chiao; V S Pantic; R A Good
Journal:  Clin Exp Immunol       Date:  1974-12       Impact factor: 4.330

5.  Combined detection of human T and B lymphocytes by rosette formation with sheep erythrocytes and zymosan-C3 complexes.

Authors:  N F Mendes; S S Miki; Z F Peixinho
Journal:  J Immunol       Date:  1974-08       Impact factor: 5.422

6.  Inhibition by C3 fragments of C3-dependent rosette formation and antigen-induced lymphocyte transformation.

Authors:  M B Pepys; A E Butterworth
Journal:  Clin Exp Immunol       Date:  1974-10       Impact factor: 4.330

7.  Fate of HL-A antigens and antibodies at the lymphocyte surface.

Authors:  S E Cullen; D Bernoco; A O Carbonara; H Jacot-Guillarmod; G Trinchieri; R Ceppellini
Journal:  Transplant Proc       Date:  1973-12       Impact factor: 1.066

8.  Specificity of human lymphocyte complement receptors.

Authors:  G D Ross; M J Polley
Journal:  J Exp Med       Date:  1975-05-01       Impact factor: 14.307

9.  Binding of soluble immune complexes to human lymphoblastoid cells. I. Characterization of receptors for IgG Fc and complement and description of the binding mechanism.

Authors:  A N Theofilopoulos; F J Dixon; V A Bokisch
Journal:  J Exp Med       Date:  1974-10-01       Impact factor: 14.307

10.  Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells.

Authors:  M Jondal; G Holm; H Wigzell
Journal:  J Exp Med       Date:  1972-08-01       Impact factor: 14.307

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  1 in total

1.  In vitro generation of human cytotoxic lymphocytes by virus. Viral glycoproteins induce nonspecific cell-mediated cytotoxicity without release of interferon.

Authors:  P Casali; J G Sissons; M J Buchmeier; M B Oldstone
Journal:  J Exp Med       Date:  1981-09-01       Impact factor: 14.307

  1 in total

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