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Literature DB >> 3003042

Nucleotide sequence of the 5' flanking region responsible for the enhancement of the expression of yeast enolase 1 gene.

H Uemura, Y Jigami, H Tanaka, N Toshimitsu, M Paterson, S Nakasato.

Abstract

Several plasmids carrying different length of the 5' flanking region of yeast (Saccharomyces cerevisiae) enolase 1 gene (ENO1) which is fused in frame to the Escherichia coli lacZ gene were constructed by recombination in vitro. Promoter activity of ENO1 was assayed by measuring beta-galactosidase activity of the fused gene product. Comparison of the promoter activity of these plasmids suggests that the sequences required for a strong promoter activity lie within the DNA segment -724 to -353 base pairs (bp) upstream from the start of ENO1 coding sequence. The nucleotide sequence of this region was determined.

Entities: Gene Species

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Year: 1985 PMID： 3003042 DOI： 10.1093/oxfordjournals.jbchem.a135345

Source DB: PubMed Journal: J Biochem ISSN： 0021-924X Impact factor: 3.387

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Cited

7 in total

1. Multiple factors bind the upstream activation sites of the yeast enolase genes ENO1 and ENO2: ABFI protein, like repressor activator protein RAP1, binds cis-acting sequences which modulate repression or activation of transcription.

Authors: P K Brindle; J P Holland; C E Willett; M A Innis; M J Holland
Journal: Mol Cell Biol Date: 1990-09 Impact factor: 4.272

2. Yeast mutants with enhanced ability to secrete human lysozyme: isolation and identification of a protease-deficient mutant.

Authors: K Suzuki; K Ichikawa; Y Jigami
Journal: Mol Gen Genet Date: 1989-10

3. Sequences within an upstream activation site in the yeast enolase gene ENO2 modulate repression of ENO2 expression in strains carrying a null mutation in the positive regulatory gene GCR1.

Authors: J P Holland; P K Brindle; M J Holland
Journal: Mol Cell Biol Date: 1990-09 Impact factor: 4.272

Nucleotide sequence of the 5' flanking region responsible for the enhancement of the expression of yeast enolase 1 gene.

1. Multiple factors bind the upstream activation sites of the yeast enolase genes ENO1 and ENO2: ABFI protein, like repressor activator protein RAP1, binds cis-acting sequences which modulate repression or activation of transcription.

2. Yeast mutants with enhanced ability to secrete human lysozyme: isolation and identification of a protease-deficient mutant.

3. Sequences within an upstream activation site in the yeast enolase gene ENO2 modulate repression of ENO2 expression in strains carrying a null mutation in the positive regulatory gene GCR1.

4. Multiple basic helix-loop-helix proteins regulate expression of the ENO1 gene of Saccharomyces cerevisiae.

5. Strains and Molecular Tools for Recombinant Protein Production in Pichia pastoris.

6. The protein factor which binds to the upstream activating sequence of Saccharomyces cerevisiae ENO1 gene.

7. Role of GCR2 in transcriptional activation of yeast glycolytic genes.